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Offlinesashasch5
jumping bean
Registered: 12/25/07
Posts: 31
Last seen: 15 years, 9 months
Inoculation Area
    #7798691 - 12/25/07 03:36 PM (16 years, 1 month ago)

I have two possible places to use as a semi-sterile area to inoculate my jars using the PF tek.

The first spot is a small nightstand I made, and I'll probably just end up wiping it down with alcohol inside and out, and running a hepa filter in the room. I'd just be working with the door open and use it kind of like a more open glovebox.

Here's some pictures of it:





The second spot is a large bathroom that I will clean top to bottom including: washing the floor with pinesol, wiping the sink, toilet, shower, and walls with a 10:1 bleach mix, and running a large hepa filter for a few hours in the room prior to inoculation. I'll be using a metal bench as my work surface.

Here's a picture of it:



So I'm asking for opinions, which one should I use if using a glovebox is out of the question?


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Invisibleim_on_a_boat
Stranger

Registered: 04/06/06
Posts: 3,950
Re: Inoculation Area [Re: sashasch5]
    #7798720 - 12/25/07 03:50 PM (16 years, 1 month ago)

i'd say the one with less carpet and closer to the inside of your house, but that's just me

i did it in my kitchen with just a candle and some rubbing alcohol and did pf jars in a giant pot on the stove (no pc) and i didn't have any contams..

that's just me though..

if i were you i'd say either that or your bathroom.. make sure you clean it hella good before you start.. and if you have one of those fans that turn on when you turn on your bathroom light, use a different light or change the filter on the fan.


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Offlinekopilcom
Navigate theseas of theshroomery!
Male

Registered: 09/23/07
Posts: 95
Last seen: 4 years, 21 days
Re: Inoculation Area [Re: im_on_a_boat]
    #7798776 - 12/25/07 04:11 PM (16 years, 1 month ago)

yes i agree with drkrobotnik less carpet and closer to the inside of your house


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Offlinekatfish
Nooblit
Male User Gallery


Registered: 07/22/06
Posts: 592
Loc: Louisiana
Last seen: 15 years, 11 months
Re: Inoculation Area [Re: kopilcom]
    #7800155 - 12/26/07 05:54 AM (16 years, 1 month ago)

i do agar in my bathroom and have no problems. smooth surface is easy to wipe down and sterilize.

kF


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08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!


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Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: Inoculation Area [Re: katfish]
    #7800160 - 12/26/07 06:02 AM (16 years, 1 month ago)

The bathroom is the worst place that as well as your kitchen. both are breading grounds for many bacteria and mold.

If they are kept really clean then it could work, but i would say that a simple glovebox would still give the best results.
I had problems mould in the beginning inoculating in my kitchen. Even after cleaning everything with bleach and detol, including spraying the air with bleach.

Its not the surfaces that are the main worry, its the air itself which has tons of spores and dirt floating around that could possible land on your materials.

Even something as simple as a carbboard box with a hole cut in the top with cling film as a see threw lid, with 2 holes for your arms will work wonders.

A glove box doesnt have to be sterile, they work on the principle of still air, so once everything has settled inside theres very little chance of things been blowin into your project.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineNibin
Getting there
Male User Gallery


Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
Trusted Cultivator
Re: Inoculation Area [Re: veda_sticks]
    #7801064 - 12/26/07 02:27 PM (16 years, 1 month ago)

Find a place with a clean surface and still air and cover your hair and mouth (to stop your breath stirring the air and crap falling onto the needles from your hair while leaning over them)

Flame the needle well and rub with alcohol to cool it down. Then inoculate. Repeat between jars.

If you want to be really safe even wipe a bit of alcohol around the inoculation holes in case you miss with the needle the first time.


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Newcomers guide-----> For all things shroomy


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