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Offlinewugg618
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Registered: 12/07/07
Posts: 3
Last seen: 15 years, 3 months
5 days after inoculation... no sign of fungus
    #7797783 - 12/25/07 09:32 AM (16 years, 1 month ago)

It's been 5 days after inoculation and I still have nothing growing. I was really care following all the step to the "pf-tek" guide I was using and im pretty sure I did everything correctly. My two concerns are 1: after you inoculate are you supposed to retape the wholes ontop of the jar? (I did). 2: I used 1ml per jar but most of the guides I read people used 10cc for only about 6 jars. Please hit me back with any ideas on why my lil guys aren't growing =( Thanks for your time and Merry Christmas everyone!


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OfflineSuperPuma
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Registered: 11/06/07
Posts: 97
Last seen: 5 years, 7 months
Re: 5 days after inoculation... no sign of fungus [Re: wugg618]
    #7797834 - 12/25/07 09:58 AM (16 years, 1 month ago)

Step 1: Wait. I'm going to assume you did multispore innoc, they can take their sweet time.

Step 2: Keep yourself entertained by making some liquid culture.


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Offlineeman113
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Registered: 10/04/07
Posts: 85
Loc: il
Last seen: 14 years, 11 months
Re: 5 days after inoculation... no sign of fungus [Re: wugg618]
    #7797861 - 12/25/07 10:10 AM (16 years, 1 month ago)

Untape the holes. It will still start without fresh air, but will slow growth as it colonizes.

1cc per jar is fine it just takes time.


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