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Offlinefluni1mg
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Registered: 12/23/07
Posts: 2
Last seen: 16 years, 12 days
Incubation way to slow - but why ?
    #7797601 - 12/25/07 07:59 AM (16 years, 1 month ago)

Hello everyone,
So here is the problem:
I used the PF-Cake method described in the Cultivation Guides. Then I inoculated the glasses with syringes (5 different cubensis strains ordered from www.azari.....) Each glas with about 1-2ml of the syringes.
The incubator is an styropor-box with a heating cable on the bottom and a temperature-controller that kept the temp at about 29 degrees constantly !
The humidity was about 80 % but I thought since the glasses are still sealed with the second tinfoil (not the ones I used for injecting) the humidity in the glasses would be OK.
So it took 14 days till the first cake was fully collonized - but only one.
There where 25 cakes. 5 of them collonized later and I put them into the growing chamber ( one cake had a not-collonized spot on the top - there was a contamination within two days !). So of 6 cakes only one had no contaminations within the grow-period and I think this is because the collonization took so long.....
1) Does anybody of you have a idea what went wrong ?
2) When a cake is fully collonized there is still the vermiculate on top (to act as a air-filter - described in the grow-guide). What do I do with that - remove? and should the original top-site stay up or should I turn the cakes ?

Thankyou for your help in advance!

Fluni


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Incubation way to slow - but why ? [Re: fluni1mg]
    #7797766 - 12/25/07 09:27 AM (16 years, 1 month ago)

After birthing, wash them under the faucet to remove the uncolonized parts of the vermiuclite filter.

Colonize jars at normal room temperature on an open shelf next time and I suspect they'll do better. Incubators, especially ones with a heat source on the bottom, tend to cook the jars, drying them out and delaying colonization.
RR


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Offlinefluni1mg
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Registered: 12/23/07
Posts: 2
Last seen: 16 years, 12 days
Re: Incubation way to slow - but why ? [Re: RogerRabbit]
    #7803015 - 12/27/07 06:05 AM (16 years, 1 month ago)

To: RogerRabbit - Thank you for your help. I'll try without that incubator the next time. But when you say faucent do you mean just regular water ? Don't I have to worry about contamination ?

Fluni


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InvisibleBridgeburner
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Registered: 09/16/06
Posts: 20,010
Re: Incubation way to slow - but why ? [Re: fluni1mg]
    #7803060 - 12/27/07 07:12 AM (16 years, 1 month ago)

once the cakes are fully colonized they can pretty much protect themselves from mold/contaminants.

hey RR what about TiT incubators for a place where the temperature is low? they are heated by water so they shouldn't cook from the bottom.


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