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grod31
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Registered: 12/06/07
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Simple monotub questions
#7788652 - 12/22/07 11:38 AM (16 years, 1 month ago) |
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So i just spent the last 5 hours preparing my first monotub.I used one and a half to two bricks of coir to 4 and a half fully colonized wbs quart jars(they took 20 sumthing days to colonize). This came out to be more spawn than i thought. It is about the same amount of coir. in total the substrate is 4 1/2". i was wondering if, since it is my first time should i skip the casing layer? second question is how long should it take for the substrate to colonize. And last if today is the 22nd would it be possible to see shrooms by new years day, or should this not even be a thought.
-------------------- Back the tape up. I need it again! Let it roll! Just as high as the fucker can go! And when it comes to that fantastic note where the rabbit bites its own head off, I want you to THROW THAT FUCKING RADIO INTO THE TUB WITH ME! Not me. It would blast you through the wall stone dead in ten seconds and they'd make me explain VVVVV Free Myco Thread
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eman113
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Registered: 10/04/07
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Loc: il
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Re: Simple monotub questions [Re: grod31]
#7788746 - 12/22/07 12:11 PM (16 years, 1 month ago) |
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It will take time to colonize, usual for me it 4-8 days. I would case if I were you. Its worth it. You won't have mushies for new year.
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thedefone
deus ex machina

Registered: 10/06/07
Posts: 1,883
Loc: Gondwana
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Re: Simple monotub questions [Re: eman113]
#7788765 - 12/22/07 12:17 PM (16 years, 1 month ago) |
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Yeah. You've got a way to go yet. Let your spawn colonize the bulk, and then start fruiting. If you are going to case that will be the time to do it.
Read me. Read me.
My experience has been that casing is worthwhile. Good luck.
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I am become death, the destroyer of worlds.
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Nibin
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Re: Simple monotub questions [Re: eman113]
#7788772 - 12/22/07 12:19 PM (16 years, 1 month ago) |
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Certainly case, and sorry to say that no mushies for new year.
Maybe if you had just cased the spawn, but even that was a long shot.
-------------------- Newcomers guide-----> For all things shroomy
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grod31
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Re: Simple monotub questions [Re: Nibin]
#7788859 - 12/22/07 12:38 PM (16 years, 1 month ago) |
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alright if im going to case my question is Can i add the casing layer to the tub outside. i live in new york and its freezing out so i figured there would be less bacteria outside then im my house even after spraying the room down, Would the freezing weather out side fuck with the substrate(put it in some sort of shock) And is the casing layer really gonna be a night and day difference? i have just waited so long to make this tub that a contam would devastate me.
-------------------- Back the tape up. I need it again! Let it roll! Just as high as the fucker can go! And when it comes to that fantastic note where the rabbit bites its own head off, I want you to THROW THAT FUCKING RADIO INTO THE TUB WITH ME! Not me. It would blast you through the wall stone dead in ten seconds and they'd make me explain VVVVV Free Myco Thread
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Nibin
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Re: Simple monotub questions [Re: grod31]
#7788896 - 12/22/07 12:50 PM (16 years, 1 month ago) |
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As long as your casing layer is sterilized and adjusted for pH with hydrated lime (and you are relatively clean, wear gloves and cover hair and mouth) risk of contamination will be very low.
-------------------- Newcomers guide-----> For all things shroomy
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derx
who run it



Registered: 05/29/03
Posts: 2,459
Loc: dx/dt
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Re: Simple monotub questions [Re: Nibin]
#7788908 - 12/22/07 12:54 PM (16 years, 1 month ago) |
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Quote:
Nibin said: As long as your casing layer is pasteurized and adjusted for pH with hydrated lime (and you are relatively clean, wear gloves and cover hair and mouth) risk of contamination will be very low.
its very unlikely to get a contam by adding a casing layer. If you have healthy mycelium, it shouldn't contam at this stage... unless maybe you like to shove your fingers up your ass and then directly handle the mycelium afterwards.....
-------------------- better living through chemistry OVERGROW the government!! it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.
Edited by derx (12/22/07 12:55 PM)
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Nibin
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Re: Simple monotub questions [Re: derx]
#7788926 - 12/22/07 12:57 PM (16 years, 1 month ago) |
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Why would you pasteurize a non nutrient containing casing layer? Pasteurisation is for bulk substrates. You can sterilize your casing no problem, especially since you are making it inhospitable by increasing the pH.
-------------------- Newcomers guide-----> For all things shroomy
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grod31
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Re: Simple monotub questions [Re: Nibin]
#7788953 - 12/22/07 01:05 PM (16 years, 1 month ago) |
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if im going to case my question is Can i add the casing layer to the tub outside. i live in new york and its freezing out so i figured there would be less bacteria outside then im my house even after spraying the room down, Would the freezing weather out side fuck with the substrate(put it in some sort of shock)
-------------------- Back the tape up. I need it again! Let it roll! Just as high as the fucker can go! And when it comes to that fantastic note where the rabbit bites its own head off, I want you to THROW THAT FUCKING RADIO INTO THE TUB WITH ME! Not me. It would blast you through the wall stone dead in ten seconds and they'd make me explain VVVVV Free Myco Thread
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Nibin
Getting there



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Re: Simple monotub questions [Re: grod31]
#7788982 - 12/22/07 01:11 PM (16 years, 1 month ago) |
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I wouldn't do it unless you have to. Inside will be fine. The substrate will be fully colonized (and so resistant to contams) and the casing will be inhospitable to contams so you are ok there.
If you follow the procedures I described above, you will be fine.
-------------------- Newcomers guide-----> For all things shroomy
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grod31
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Re: Simple monotub questions [Re: Nibin]
#7789020 - 12/22/07 01:17 PM (16 years, 1 month ago) |
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thank you very much this info will be noted this question is kinda unrelated since these jars took 20+ days is it more likely that it will take longer then normal for the substrate to colonize aswell. or do the colonization time of the jars and the substrate have nothing to do with each other.
-------------------- Back the tape up. I need it again! Let it roll! Just as high as the fucker can go! And when it comes to that fantastic note where the rabbit bites its own head off, I want you to THROW THAT FUCKING RADIO INTO THE TUB WITH ME! Not me. It would blast you through the wall stone dead in ten seconds and they'd make me explain VVVVV Free Myco Thread
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Nibin
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Re: Simple monotub questions [Re: grod31]
#7789176 - 12/22/07 02:01 PM (16 years, 1 month ago) |
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If the water content is good, your mycelium will spread like wildfire.
-------------------- Newcomers guide-----> For all things shroomy
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derx
who run it



Registered: 05/29/03
Posts: 2,459
Loc: dx/dt
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Re: Simple monotub questions [Re: Nibin]
#7789215 - 12/22/07 02:20 PM (16 years, 1 month ago) |
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Quote:
Nibin said: Why would you pasteurize a non nutrient containing casing layer?
why would you STERILIZE a non nutrient containing casing layer?
There is simply no benefit to sterilizing a casing layer and there is a benefit to pastuerizing it.
Quote:
Nibin said: Pasteurisation is for bulk substrates.
No, Pasteurization is a process that kills off harmful organisms. People use pasteurization for preparing bulk substrates. Most of these people also use pasteurization for preparing casing mix. It just doesn't make any sense to sterilize a casing mix.
Quote:
Nibin said: You can sterilize your casing no problem, especially since you are making it inhospitable by increasing the pH.
Are you trying to suggest you a contaminate cannot grow in a pH of 7-8?
-------------------- better living through chemistry OVERGROW the government!! it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.
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Nibin
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Re: Simple monotub questions [Re: derx]
#7789265 - 12/22/07 02:37 PM (16 years, 1 month ago) |
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From the good old 50/50 casing tek.
Quote:
Tips on sterilizing and pasteurizing substrate: There has been a lot of discussion on whether it's better to sterilize the casing soil or not. In my friend's studies, he has found that sterilizing is better. Some people like to pasteurize it. That's OK, but he prefers to sterilize it. My friend buys filter patch bags from Myco Supply or Fungi Perfecti or various other vendors. These are large autoclavable bags (meaning they can go in the pressure canner and survive the heat without melting) with a filter on them for gas exchange. Although for sterilizing the casing soil, the patch does not matter. Basically after mixing up all the dry ingredients, (pulling out all the sticks from the peat moss) for the 50/50 mix, add in your water. Mix really good, have your filter patch bag handy, squeeze out most of the water from the casing mix. Squeeze so its just slightly dripping then put it into the bag. Fill the bag about 3/4 full. Put this in your pressure canner and be sure to keep it away from the sides of the pressure cooker. Sometimes the filter patch bag will melt if it touches the sides. Sterilize at 15 p.s.i for 45 minutes.
I think it makes much more sense to sterilize. Sterilizing is just pop in the PC and forget, no mucking about with thermometers and stuff.
It also makes sense to sterilize as a sterile and pH adjusted casing mix will have a very hard time contaminating. The mycelium can tolerate the alkalinity and also has access to the food source below the casing. Any spores landing on the casing mix will be hard pressed to take it over.
-------------------- Newcomers guide-----> For all things shroomy
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