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Fraggin
Multi-Faceted



Registered: 01/05/05
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Osmotic Pressure Thresholds.
#7781834 - 12/20/07 02:15 PM (16 years, 1 month ago) |
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At what point would cytolysis occur on mycelium while under hydrostatic pressure from a 'dunk-n-roll'.
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SuperPuma
Automation Nut



Registered: 11/06/07
Posts: 97
Last seen: 5 years, 7 months
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Re: Osmotic Pressure Thresholds. [Re: Fraggin]
#7783891 - 12/20/07 11:01 PM (16 years, 1 month ago) |
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What is the airspeed velocity of an unladen swallow?
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Crake
Stranger


Registered: 12/04/07
Posts: 194
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Re: Osmotic Pressure Thresholds. [Re: Fraggin]
#7783908 - 12/20/07 11:05 PM (16 years, 1 month ago) |
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Quote:
Fraggin said: At what point would cytolysis occur on mycelium while under hydrostatic pressure from a 'dunk-n-roll'.
That is a good question... What is your intent?
-------------------- MANGO Snowman wakes before dawn. He lies unmoving, listening to the tide coming in, wave after wave sloshing over the various barricades, wish-wash, wish-wash, the rhythm of heartbeat. He would so like to believe he is still asleep.
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MYSTIQUE
Say Hi to the elves for me.




Registered: 04/28/07
Posts: 1,764
Loc: Canada
Last seen: 5 years, 4 months
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Re: Osmotic Pressure Thresholds. [Re: Crake]
#7783914 - 12/20/07 11:06 PM (16 years, 1 month ago) |
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The airspeed velocity of an unladen swallow depends on many factors and parameters. For example, an African Swallow would tend to travel at a different velocity than an European Swallow. Furthermore, their flight patterns are different, so their instantaneous velocity at a given point will almost always be different. However, technological advances have given way to being able to use swallows to transport husked coconuts across large distances, enabling us to use coconuts on a daily basis, provided we have a large contingent of willing swallows ready to make a nonstop convoy for X amount of time, or however long we wish. Swallows can also be modified, as attaching a 50 newton rocket propulsion system on a 1 kg swallow will result in an acceleration of 50 m/s(2), which is of course negating any air resistance, since swallows have been recorded as outer space fliers. I hope i cleared any doubts about this matter.
-------------------- Dont know what the fuck I just said? READ THIS http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary I ain't a hippy but I'm covered in dirt Sippin lots of mushroom tea in a tye-dye shirt Chasin' the Grateful Dead, no shoes on my feet Beggin' in the parking lot for something to eat, DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!
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Fraggin
Multi-Faceted



Registered: 01/05/05
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Re: Osmotic Pressure Thresholds. [Re: SuperPuma]
#7783918 - 12/20/07 11:07 PM (16 years, 1 month ago) |
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ummmm.... 24 miles per hour, but why are you hijacking my thread?
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Nibin
Getting there



Registered: 11/29/05
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Re: Osmotic Pressure Thresholds. [Re: SuperPuma]
#7783922 - 12/20/07 11:08 PM (16 years, 1 month ago) |
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First of all, hydrostatic and osmotic pressure are different things.
What are you asking?
Will the water pressure from being submerged cause cytolysis?
or
Will the lower salt concentration of say distilled water used to dunk cause osmotic imbalance and cytolysis?
in either case the answer is no, as in a) you can't submerge deep enough to create that kind of pressure and b) because mycelium has regulation functions relating to this.
-------------------- Newcomers guide-----> For all things shroomy
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Fraggin
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Registered: 01/05/05
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Re: Osmotic Pressure Thresholds. [Re: Fraggin]
#7783965 - 12/20/07 11:19 PM (16 years, 1 month ago) |
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Quote:
Fraggin said: At what point would cytolysis occur on mycelium while under hydrostatic pressure from a 'dunk-n-roll'?
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SuperPuma
Automation Nut



Registered: 11/06/07
Posts: 97
Last seen: 5 years, 7 months
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Re: Osmotic Pressure Thresholds. [Re: MYSTIQUE]
#7784034 - 12/20/07 11:40 PM (16 years, 1 month ago) |
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Bahahaha, yes!
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Crake
Stranger


Registered: 12/04/07
Posts: 194
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Re: Osmotic Pressure Thresholds. [Re: SuperPuma]
#7784182 - 12/21/07 12:48 AM (16 years, 1 month ago) |
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Fraggin, your questions have me asking questions. I understand your query, yet I am curious as to why you are asking this... what is you intent?
-------------------- MANGO Snowman wakes before dawn. He lies unmoving, listening to the tide coming in, wave after wave sloshing over the various barricades, wish-wash, wish-wash, the rhythm of heartbeat. He would so like to believe he is still asleep.
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Crake
Stranger


Registered: 12/04/07
Posts: 194
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Re: Osmotic Pressure Thresholds. [Re: Crake]
#7784187 - 12/21/07 12:53 AM (16 years, 1 month ago) |
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... sigh, if I had to answer this I'd have to ask first what depth you intend to dunk at. If I have any grasp at all of the mechanics involved here I would assume that there is a depth factor involved, not so much a time parameter, perhaps both. In fact you'd involve both factors in different instances, or just depth in others. Again, allow me to ask... WHY do you ask?
-------------------- MANGO Snowman wakes before dawn. He lies unmoving, listening to the tide coming in, wave after wave sloshing over the various barricades, wish-wash, wish-wash, the rhythm of heartbeat. He would so like to believe he is still asleep.
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SuperPuma
Automation Nut



Registered: 11/06/07
Posts: 97
Last seen: 5 years, 7 months
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Re: Osmotic Pressure Thresholds. [Re: Crake]
#7784219 - 12/21/07 01:13 AM (16 years, 1 month ago) |
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Seriously now, are you planning on taking these cakes with you on your way to the bottom of your chilled, 100 ft, non-chlorinated pool?
Doesn't common sense say you don't need any kind of pressure to saturate cakes to their max capacity? The need for that data is absolutely confounding.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
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Re: Osmotic Pressure Thresholds. [Re: SuperPuma]
#7784404 - 12/21/07 03:03 AM (16 years, 1 month ago) |
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RR has always suggested that dunking should be done under some pressure,.
Thats why when you watch his videos the are kept submerged with a plate so there is a little water pressure which helps rehydrate the cake.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
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Re: Osmotic Pressure Thresholds. [Re: veda_sticks]
#7784431 - 12/21/07 03:16 AM (16 years, 1 month ago) |
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Gosh, I'm going to feel mighty stupid if I'm wrong about this, but I think the only reason I put something on top of my cakes is to keep them completely submerged under water. I have yet to come across a cake that didn't want to float! I thought it was as simple as that!
Quote:
RR has always suggested that dunking should be done under some pressure,.
I think it's because it takes a little opposing pressure to counteract the buoyancy effect produced by trapped air and less dense vermiculite. Once again. I'm feeling a little stupid.
--------------------
AMU Q&A thread.
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SuperPuma
Automation Nut



Registered: 11/06/07
Posts: 97
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Re: Osmotic Pressure Thresholds. [Re: The shroomy 1]
#7784686 - 12/21/07 07:19 AM (16 years, 1 month ago) |
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Shroomy 1 has is it completely right.
Veda, not quite.
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Fraggin
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Re: Osmotic Pressure Thresholds. [Re: SuperPuma]
#7784806 - 12/21/07 08:29 AM (16 years, 1 month ago) |
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The PRIMARY reason for submersing a cake in water at 100% colonization ,or between flushes, is NOT to rehydrate the substrate.
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laten
Stranger

Registered: 11/08/07
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Last seen: 14 years, 8 months
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Re: Osmotic Pressure Thresholds. [Re: Fraggin]
#7784840 - 12/21/07 08:43 AM (16 years, 1 month ago) |
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I think it is, though.
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SuperPuma
Automation Nut



Registered: 11/06/07
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Re: Osmotic Pressure Thresholds. [Re: laten]
#7784948 - 12/21/07 09:37 AM (16 years, 1 month ago) |
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Fraggin, would you care to offer a PRIMARY reason to dunking in order to replace the PRIMARY reason you've so gracefully ushered out the door?
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Fraggin
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Re: Osmotic Pressure Thresholds. [Re: SuperPuma]
#7784970 - 12/21/07 09:48 AM (16 years, 1 month ago) |
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Quote:
SuperPuma said: Fraggin, would you care to offer a PRIMARY reason to dunking in order to replace the PRIMARY reason you've so gracefully ushered out the door?
The PRIMARY reason for dunking a fully colonized substrate is to rehydrate the mycelial cells.
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eman113
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Registered: 10/04/07
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Re: Osmotic Pressure Thresholds. [Re: Fraggin]
#7785007 - 12/21/07 10:09 AM (16 years, 1 month ago) |
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You are right.
Are u going to add pressure to a vessel with water and the cake to hydrate faster or creating more pressure though the deepth of the cake the water to decrease dunk time?
Its a interesting idea.
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Fraggin
Multi-Faceted



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Re: Osmotic Pressure Thresholds. [Re: eman113]
#7785033 - 12/21/07 10:20 AM (16 years, 1 month ago) |
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I was considering retrofitting a spare PC with a schraeder valve to use as a pressurized dunking vessel to see if it would decrease the time needed for a dunk as well as pass soluable and/or semi-soluable nutrients across the cell membranes.
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