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Invisibletony8404
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what is with LC's
    #7770607 - 12/17/07 05:35 PM (16 years, 1 month ago)

i do not understand what i am doing wrong with my lc's...
this is the 4th one i have tried and no luck!!!

first i thought it was cause of carmelization, i finally got that out of the picture... this last lc i made was clear 100ml distilled water and 3ml of karo... i inoculated with spores and placed the baby bottle on top of a radiator in my bathroom i have an old boiler system in my house. the temp is usually set at around 70-75 degrees...
do you think maybe sitting on top of the radiator is too hot for it? i do see alot of condisation in the bottle at the top where there is no water...

i mean is making an lc this hard... the last time i did this was my second try at it my first time i overcooked them, the next time i got it right... but now i cant get close... anyone know of a good tek maybe other the the baby bottle tek????


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Offlinesproket13
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Re: what is with LC's [Re: tony8404]
    #7770611 - 12/17/07 05:36 PM (16 years, 1 month ago)

....how long you steralize for. i pressure cook mine at 15 psi for 15 minutes. then take it out


no problems


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InvisibleBUDDHA_702
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Re: what is with LC's [Re: tony8404]
    #7770621 - 12/17/07 05:39 PM (16 years, 1 month ago)

What media ratio are you using?
Add something to the karo, like some Sugar In The Raw
I PCed at 10psi for 20min


Edited by BUDDHA_702 (12/17/07 05:40 PM)


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Offlinesoul_psychonaut
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Re: what is with LC's [Re: sproket13]
    #7770622 - 12/17/07 05:39 PM (16 years, 1 month ago)

i agree with sproket on the sterilization time.

i personally use 5grams of raw sugar to 220ml of water. I use jars with self healing innoc ports.

works like a charm


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Offlinesproket13
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Re: what is with LC's [Re: soul_psychonaut]
    #7770630 - 12/17/07 05:41 PM (16 years, 1 month ago)

i use exactly what he did


3mm karo/ 100 ml water


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InvisibleFooMan
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Re: what is with LC's [Re: tony8404]
    #7770662 - 12/17/07 05:48 PM (16 years, 1 month ago)

There are a few "teks" in the archives.

http://www.shroomery.org/forums/postlist.php/Cat/0/Board/59

There's not much to it. Use 4cc's of Karo to about 100cc's of water sterilized in any autoclavable container with an injection port and filter and inject your spores/LC. Carmelization won't stop growth. There may be something else you're doing wrong.


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Invisibletony8404
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Re: what is with LC's [Re: FooMan]
    #7770817 - 12/17/07 06:21 PM (16 years, 1 month ago)

well, i use distilled water... i pc'ed 15psi for 15 minutes...

maybe i can try 10psi 20 minutes... is raw sugar better then karo?


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Invisibletony8404
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Re: what is with LC's [Re: tony8404]
    #7770825 - 12/17/07 06:22 PM (16 years, 1 month ago)

buddha_702 how much karo and raw sugar you using together?


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Invisibletony8404
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Re: what is with LC's [Re: tony8404]
    #7770898 - 12/17/07 06:34 PM (16 years, 1 month ago)

also, in the baby bottle tek i was using... it does not say anything about getting the distilled water hot first then add the karo and shake it up to dissolve, is that step neccessary???


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InvisibleBUDDHA_702
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Re: what is with LC's [Re: tony8404]
    #7770943 - 12/17/07 06:45 PM (16 years, 1 month ago)

You would want to use a karo+ LC.
Karo & sugar in the raw
2-4% media ratio

I use 4% that means 4% of 100ml
so 3ml of karo and of 1tsp. of sugar in the raw

1 teaspoon = 4.9 milliliters


Edited by BUDDHA_702 (12/17/07 06:48 PM)


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Invisibletony8404
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Re: what is with LC's [Re: BUDDHA_702]
    #7771033 - 12/17/07 07:13 PM (16 years, 1 month ago)

really oh thanks guys... i didnt read up on the raw sugar thing yet... will this help with successful lc's?? or is the raw sugar just to help carmelization??

also, which is better for sterilization??? 15 psi for 15 min,or 15 psi for 20 min or 10 psi 20 min ?

once this is done pcing and has cooled over night... i was just thinking maybe i have not put enough syringe spores into my lc's

i think my last lc that is still in a trial period i added 2cc of spores but before that i think i may haave only used 1cc ... does this make a difference in failure or what not??

s


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InvisibleFooMan
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Re: what is with LC's [Re: tony8404]
    #7771061 - 12/17/07 07:18 PM (16 years, 1 month ago)

Personally, I never PC more than 10 minutes at 15psi to avoid carmeilization, but again carmelization WON'T stop growth. The main reason to avoid it is so that the LC is clear and contaminants are more easily identifiable. Something else is wrong here.


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Offlinesproket13
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Re: what is with LC's [Re: FooMan]
    #7771077 - 12/17/07 07:22 PM (16 years, 1 month ago)

i dont use sugar in the raw. karo ALONE works fine.

no need to add extra.


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Invisibletahoe
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Re: what is with LC's [Re: FooMan]
    #7771085 - 12/17/07 07:25 PM (16 years, 1 month ago)

hahaha. Just because the temp is set at 75 degrees in the house doesn't mean that the radiator will not get hotter. I would think that thing would be over 100 degrees. Is it warm to the touch? If so then it is probably 90+. if it is hot to the touch then it is closer to 110. If it is scolding then its 150+

Why would you want to heat it above room temp??? You guys with your worrying about heat. Room temp is fine. Put the lc in the cupboard next to the plates. It will be fine


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
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Teh=The
I need to proofread


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OfflineRogerRabbitM
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Re: what is with LC's [Re: sproket13]
    #7771088 - 12/17/07 07:25 PM (16 years, 1 month ago)

I'll bet that radiator gets too hot. Put the jars on a shelf well above it.
RR


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InvisibleFooMan
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Re: what is with LC's [Re: sproket13]
    #7771105 - 12/17/07 07:30 PM (16 years, 1 month ago)

Karo, turbinado, malt/dextrose. It's all good. If Karo didn't work for this guy, I'm not sure why folks are telling him to add more shit into the mix. :confused:


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InvisibleFooMan
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Re: what is with LC's [Re: RogerRabbit]
    #7771115 - 12/17/07 07:32 PM (16 years, 1 month ago)

Tahoe, RR- I think you guys hit the nail on the head. I missed the part about the radiator.


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InvisibleBUDDHA_702
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Re: what is with LC's [Re: FooMan]
    #7771283 - 12/17/07 08:05 PM (16 years, 1 month ago)

Karo is the worst thing to make LC of of add turbinado sugar makes a better LC.
+
MYC like more than one food source most people use 3-4 different kinds of media.


Edited by BUDDHA_702 (12/17/07 08:06 PM)


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Invisibletony8404
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Re: what is with LC's [Re: BUDDHA_702]
    #7771356 - 12/17/07 08:18 PM (16 years, 1 month ago)

for the love of god it has to be the radiatoer rr, tahoe and foomanshroom thanks ... ahhhh i can so kick myself right now... oh geez thats it i need a beer and a bowl smoke.. lol.. i wanna cry.. heheheh. i canot believe i thought that was okay. i even bought a 60 dollar on a infarred hand held thermometer.. im gonna put it to use..........................thank you guys


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Invisibletahoe
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Re: what is with LC's [Re: tony8404]
    #7771374 - 12/17/07 08:20 PM (16 years, 1 month ago)

if you didnt smoke so much weed then you might have realized that the radiator is hot


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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