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Offlinewhereismymind
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wbs shaked after inoc starts colonizing at the bottom ??
    #7766801 - 12/16/07 06:51 PM (16 years, 1 month ago)

hey there , my white millet jars which i mentioned that i have cooked for 1h 50 min 15 psi and were brown somehow (you can see the pictures in this thread http://www.shroomery.org/forums/showflat.php/Cat/0/Number/7750825/an/0/page/0)
are inocced 4 days ago now , when i checked today to see the progress i've seen the germination only started at the bottom of every jar , can this be a sign that these millets lost their water or sth during pc ing (millet was soaked for 18 h and drained then loaded to a pc with weight , after 1.50 mins of cookin some portion of the water inside the pc was evaporated ) So does it have a chance that during the process the millet dried up or something anyone experienced these kind of things ? what could be the cause of this =)
thanks , peace .


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In my own summer , there is no crowd in the streets and no sun...


Edited by whereismymind (12/16/07 06:53 PM)


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Offlinewhereismymind
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: whereismymind]
    #7767041 - 12/16/07 07:42 PM (16 years, 1 month ago)

anyone ?


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Invisibletahoe
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: whereismymind]
    #7767293 - 12/16/07 08:39 PM (16 years, 1 month ago)

1. Why the hell would you cook them for 110 minutes????? Did you figure out why they are brown at the bottom???

2. The spores germinated at the botom because gravity pulled the liquid down to the botom.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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Offlinewhereismymind
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: tahoe]
    #7768442 - 12/17/07 07:30 AM (16 years, 1 month ago)

they are not only brown at the bottom (they are generally brown ) they were not in contact with the water all the jar is sterilized with vapor so no burnt kernels at the bottom .

well actually i was going to cook em for 90 mins then i left kitchen and was surfin the internet when i realized it has been too much!! so what do you think is there still enough water in these millets to colonize ?


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In my own summer , there is no crowd in the streets and no sun...


Edited by whereismymind (12/17/07 07:32 AM)


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Offlinewhereismymind
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: tahoe]
    #7768556 - 12/17/07 08:39 AM (16 years, 1 month ago)

i hope the gravity pulled the liquid of the syringe (spore water down ) and these millets still have water left in them =(


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Offlinedrwatson
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: whereismymind]
    #7768682 - 12/17/07 09:40 AM (16 years, 1 month ago)

Sometimes my WBS jars colonize on the bottom, sometimes in the middle, and other times at the top.

That millet packed into a little jar will both spread and catch bits of your solution. Sometimes it makes it all the way to the bottom of the jar and sometimes it doesn't.

I don't usually shake mine until they're around 33-45% or so if I shake at all. But that's just me I'm not in a hurry.


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Offlinewhereismymind
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: drwatson]
    #7768694 - 12/17/07 09:45 AM (16 years, 1 month ago)

thank you for your reply =) it is such a relief cause i was thinking because i cooked them longer accidentaly , thought that maybe water in kernels evaporated , but since you are also facing the same situation i guess no need to worry , let them start colonizing the bottom first (even though they were shaked =) i will shake them once more when they are %30 thanks =)


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Invisibletahoe
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: whereismymind]
    #7768723 - 12/17/07 09:56 AM (16 years, 1 month ago)

the millet still has enough water in it to coloninze.

Why would you pressure cook the jars for 90 mins?


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinewhereismymind
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: tahoe]
    #7769900 - 12/17/07 02:23 PM (16 years, 1 month ago)

Well i have had bad results while in my country , and i had a shitty pc and im sure it didn't even reach 112 c anyway i had 1 batch of rye (3 jars ) and 2 batches of wbs (3 jars again ) an they all failed . Reading some users opinion about being on the safe side grains can be cooke 90 mins , this time even though my pressure cooker could reach 15 psi wanted to cook for 90 mins to be sure that this batch everything goes ok , ruined everything when started pc ing them and going back to my room reading shroomery , when i checked the time it was 110 mins =( ,

I would be pleased if it is ok to just cook for an hour without facing any contamination (i would like to know also for rye is it ok to cook for 1 hour at 15 psi ??) , it is easier for me =)


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OfflineFraggin
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: whereismymind]
    #7769920 - 12/17/07 02:28 PM (16 years, 1 month ago)

Quote:

whereismymind said:
Well i have had bad results while in my country ,




I don't think this hobby is country specific.

Quote:


and i had a shitty pc and im sure it didn't even reach 112 c




A good method of sterilization is key.
Quote:


anyway i had 1 batch of rye (3 jars ) and 2 batches of wbs (3 jars again ) an they all failed .




Failing is part of this hobby in the beginning, and even for veterans. Success is not a gurantee.
Quote:


Reading some users opinion about being on the safe side grains can be cooke 90 mins , this time even though my pressure cooker could reach 15 psi wanted to cook for 90 mins to be sure that this batch everything goes ok ,




Opinions Vary. 90 minutes at 15 psi, including the time it takes to get to 15psi is a long time to cook grains without them turning to mush
Quote:


ruined everything when started pc ing them and going back to my room reading shroomery , when i checked the time it was 110 mins =( ,




Never leave a pressure cooker unattended

Quote:


I would be pleased if it is ok to just cook for an hour without facing any contamination




sounds fine to me. I cook wbs for 1hr at 10psi with good luck.
Quote:


(i would like to know also for rye is it ok to cook for 1 hour at 15 psi ??) , it is easier for me =)




If rye is easier for you, why mess with wbs?


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OfflineCheesekiller
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: Fraggin]
    #7770069 - 12/17/07 03:15 PM (16 years, 1 month ago)

I always PC for 90 min @ 15PSI. Usually fully colonized by 7th day. Most important thing I've found is to shake the jars while they are still cooling from PCing or they gum up real bad. Next day knock em up. Two days later shake again. (I use an incubator). The grain in that picture looks just like mine always look except that I usually have some left over black sunflower seeds.


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Offlinewhereismymind
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: Fraggin]
    #7770094 - 12/17/07 03:25 PM (16 years, 1 month ago)

well actually that 1hours 50 mins is including the 13 mins to get to 15 psi also =) , i would like to have rye but here they don't sell rye i had to order it online and the online company told that because they are busy these days it could take up to 15 days to receive my order so have 3 jars of wbs and waiting for my rye right now , and after i have my rye i don't think i ll be messing around with millet it is too messy with those small things spreading all around

And this is the photo of the overcooked millet (mostly white and yellow)

u can see how they are now hope they colonize well =) i just thought it is funny since i shaked after inoculation they still start colonizing on the bottom .


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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: whereismymind]
    #7770625 - 12/17/07 05:41 PM (16 years, 1 month ago)

dude. everything is fine!!! i knocked up some jars 4 days ago and dont see anything. its my first time using spores in a while. and it does take longer. but i woulda expected to see more growth. i see small white.

normally mine start ont he bottom. i then break it up and shake it around, spread it out...then 2 days later. whole things done


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Offlinewhereismymind
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: sproket13]
    #7770704 - 12/17/07 05:57 PM (16 years, 1 month ago)

the most interesting thing i find is i " shaked" after inoculation that is why i opened this thread im not concerned about the germination rate , i was just curious even though i "SHAKED" after inoculation why do they start colonizing at the bottom =) thanks for your comments peace ..


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Invisibletahoe
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: whereismymind]
    #7771041 - 12/17/07 07:15 PM (16 years, 1 month ago)

they colonize at the bottom. Gravity pulls things down. Liquid is affected my gravity. Spores are in the liquid. Spores are affected my gravity.
So gravity, this is why they colonized at the bottom.
Once it germinates then shake


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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OfflineCheesekiller
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: tahoe]
    #7771138 - 12/17/07 07:36 PM (16 years, 1 month ago)

You shouldn't shake after inoculation.

Wait a couple of days, for the myc to catch itself. Its almost like males and females getting together. Once you see a tab bit of growth shake the piss out of it, and you will be onto a fierce growth rate.

Search about it a little if you don't believe me, but shaking right after inoculation actually stalls the myc out.

-CK


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Invisibletahoe
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: Cheesekiller]
    #7771197 - 12/17/07 07:48 PM (16 years, 1 month ago)

yeah you can shake after inoculation .
Even if you shake the majority of it will run back to the bottom.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


Edited by tahoe (12/17/07 10:37 PM)


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Offlinesproket13
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: tahoe]
    #7771326 - 12/17/07 08:13 PM (16 years, 1 month ago)

...so lay them on their sides.



makes this whole "male female" thing stated above more likely to happen. i enjoy mating while laying. not standing up  :smile:


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Offlinewhereismymind
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: tahoe]
    #7771524 - 12/17/07 08:47 PM (16 years, 1 month ago)

I have read before in one of rr's posts describing how shaking after inoc slows things down by seperating spores while they were about to choose a mate etc i have read it well before ... all my jars before were inocced considering this , this is the only time i have done it just to try and see .

wow actually if i thought it was a stupid question i wouldn't ask it , besides i used the search function and gone as deep as i can (keywords : wbs jars start colonizing at bottom ; colonizing starts at the bottom ; etc... )
Actually i wouldn't come here asking question if the cooking time wasn't off because of it i'm relating what i see with that . and on my other jars i didn't experience anything like this , that was why i was asking this ,okay from now on i am not asking any questions to anyone (it is what this forum is about anyway ) since someone might not like your question . Anyway i tried to find a solution in the database but couldn't and asked ..
peace =)


--------------------
In my own summer , there is no crowd in the streets and no sun...


Edited by whereismymind (12/17/07 09:58 PM)


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OfflineNibin
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Re: wbs shaked after inoc starts colonizing at the bottom ?? [Re: whereismymind]
    #7773934 - 12/18/07 02:01 PM (16 years, 1 month ago)

Quote:

whereismymind said:
Well i have had bad results while in my country , and i had a shitty pc and im sure it didn't even reach 112 c me =)




Remember to vent your PC or it won't reach the right temperatures


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