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Offlinerexmundi
Stranger

Registered: 08/10/07
Posts: 314
Last seen: 14 years, 2 months
puzzled by colonization
    #7765766 - 12/16/07 02:18 PM (16 years, 1 month ago)

Ok so a while ago I made up a batch of jars for my second grow. This time I inoculated using my LCs instead of a multispore syringe.

First I made six jars, roughly following the substrate guide for the pf tek. I PC'd, and inoculated them, and put them in my incubator. They started showing mycelium colonization fairly quickly. I must at this point note that I am using one pint jars. I bought them accidently for my first grow and used them anyway, they take a little longer to colonize but they still worked. Anyway so these jars were colonizing in my incubator for a few days when i decided to do up three more jars. Same situation only this time I was a little drunk when I made them up, so I may have messed up at some point.

Anyway, after a few days, the colonizing of the first six jars slowed almost to a stop, even though they sit in an incubator that is about 79 degress. The next three have not contamed, but there is absolutely no growth in them at all. So I figured patience was key, flipped the first six for air circulation and waited.

I just checked on them again and the three still have nothing, no mycelium, no contams, nothing. The other six have not showed any signs of progress and indeed, the substrate seems like it is crumbling inside the jars. The mycelium is not fluffy white, but looks...I dunno...dry or something. My first grow I had problems with FAE in the fruiting process and the mycelium cakes dried right up and contracted, that is what it looks like inside my jars.

I don't know what I did wrong. Maybe I wasn't paying attention to substrate ratios? Why did it all of a sudden stop colonizing, or in some cases never start?

I'm in the middle of exams right now, so I'm not going to do anything drastic right now. Afterwards I might try and salvage the mycelium by making a casing or something. They seem pretty hopeless to me right now, next time I might have to get some smaller jars and pay more attention to the substrate mix, but I still don't know what went wrong. If anything, when mixing my substrate, I was liberal with the BRF rather than stingy.

Anyway, sorry for the long post. Let me know what you think might be the case. Next study break I might be ambitious and try and post pics if that would help. Thanks in advance for your replies.


--------------------
"I Love Democracy"
-Emporer Palpatine


Fuck the system.


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Offlinejlw
New guy
Male

Registered: 12/09/07
Posts: 62
Loc: St. Louis
Last seen: 14 years, 6 months
Re: puzzled by colonization [Re: rexmundi]
    #7765795 - 12/16/07 02:25 PM (16 years, 1 month ago)

How long ago was all this? Sorry if I am missing it.


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OfflineNibin
Getting there
Male User Gallery


Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
Trusted Cultivator
Re: puzzled by colonization [Re: jlw]
    #7766467 - 12/16/07 05:23 PM (16 years, 1 month ago)

Was your LC good? have you grown anything with it successfully?

Another q. What amount of water did you use in the mix? How much LC did you inoculate with?


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Newcomers guide-----> For all things shroomy


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OfflineYossarian22
Stranger
Registered: 09/12/07
Posts: 415
Last seen: 9 years, 1 month
Re: puzzled by colonization [Re: Nibin]
    #7766486 - 12/16/07 05:31 PM (16 years, 1 month ago)

I'm still a newbie myself, so don't take my word for it unless it's confirmed by someone more knowledgeable than myself, but my guess would be that you made the mixture too wet. Besides, it seems as though cubes have a hard time colonizing BRF/verm mixtures in bigger containers. You may want to take out the six containers, rinse off the uncolonized remainder and just use that.


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Offlinerexmundi
Stranger

Registered: 08/10/07
Posts: 314
Last seen: 14 years, 2 months
Re: puzzled by colonization [Re: Yossarian22]
    #7766988 - 12/16/07 07:28 PM (16 years, 1 month ago)

Well I haven't completed a grow with the LC. It is also in a one pint jar. It was made with 3 or 4 tablespoons of honey, the rest is water. I sterilized and inoculated it and there is a pretty big blob of mycelium in it. When I inoculated the jars I just used a syringe to suck up a bit of the mycelium and put a few "blobs" in each one.

This has been about 3-5 week process I think.

The wetness thing strikes a cord, I do recall having some difficulty when mixing up the substrate now that I think about it. I do wonder about those other three jars though.


--------------------
"I Love Democracy"
-Emporer Palpatine


Fuck the system.


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