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OfflinePoisonedV
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Too many spores?
    #7764226 - 12/16/07 12:17 AM (16 years, 1 month ago)

I put about 4cc's of spores into 1 jar. I did it on accident, the syringe was looser than normal. Anyway, will this make it grow faster, make it grow worse, or does it not matter?


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Lazy...


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InvisibleCrake
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Registered: 12/04/07
Posts: 194
Re: Too many spores? [Re: PoisonedV]
    #7764263 - 12/16/07 12:27 AM (16 years, 1 month ago)

I would think that it wouldn't have to much impact on moisture content, although 4cc's is more than the normal 1. Not by much. However I would imagine the extra spores would do anything but hurt. :2cents:


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MANGO

Snowman wakes before dawn. He lies unmoving, listening to the tide coming in, wave after wave sloshing over the various barricades, wish-wash, wish-wash, the rhythm of heartbeat. He would so like to believe he is still asleep.


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Invisibleflavoraid
now with twicethe ketamine andopiates!
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Re: Too many spores? [Re: Crake]
    #7764283 - 12/16/07 12:33 AM (16 years, 1 month ago)

IF the moisture content isn't thrown off I'd assume it would only colonize faster.


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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OfflinePoisonedV
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Re: Too many spores? [Re: flavoraid]
    #7764293 - 12/16/07 12:36 AM (16 years, 1 month ago)

Ok so either the spores moisturized the injection points a whole lot or the things are starting to colonize already. I am seeing some white growth that looks to pale to be just moisture. =D


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OfflinePoisonedV
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Re: Too many spores? [Re: PoisonedV]
    #7765530 - 12/16/07 01:10 PM (16 years, 1 month ago)

Another update, these things are growing, it's definitely not moisture. Awesome.


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Lazy...


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Offlinec0_hush
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Re: Too many spores? [Re: PoisonedV]
    #7765589 - 12/16/07 01:29 PM (16 years, 1 month ago)

If you do the math, each 1/2 pint jar gets around 60cc's of water mixed in with verm + brf so adding 4cc's of solution would most likely be insignificant.


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OfflineTheravadaDreams
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Re: Too many spores? [Re: c0_hush]
    #7766464 - 12/16/07 05:23 PM (16 years, 1 month ago)

I use 2 cc's per jar for 1/2 Pint cakes.


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"Believe nothing, no matter where you read it, or who said it, no matter if I have said it, unless it agrees with your own reason and your own common sense." -Siddhartha Guatama a.k.a Buddha.

MonsterMitch Mono-Tub.



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OfflineNibin
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Re: Too many spores? [Re: TheravadaDreams]
    #7766483 - 12/16/07 05:30 PM (16 years, 1 month ago)

1 cc is more than enough. I get 12 jars out of a 10cc syringe and get growth in around 3 days without even using an incubator.


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Offlineveda_sticks
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Re: Too many spores? [Re: Nibin]
    #7767412 - 12/16/07 09:11 PM (16 years, 1 month ago)

Using more solution poses the risk of increasing the moisture content of the substrate. They should still grow fine as long as your moisture content wasnt too wet.

U may get more substrains growing giving a wider varience in genetics.

It may work out in your favour or not. Its quite random so theres no telling how it will go.

As long as your substrate and sterile technique is good, it will grow, the only variables left is genetics (and your enviroment)


--------------------

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Offlineoysterguy63
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Re: Too many spores? [Re: veda_sticks]
    #7767556 - 12/16/07 09:46 PM (16 years, 1 month ago)

The added moisture is pretty small...but if the moisture in your spawn was a little high it might have an impact...I recently had the same thing happen and my moisture content was already a tad high. I had issues and ended up trashing that particular jar's worth of sub....but if you stuck to your recipe properly those extra 3 cc's...probably not a big deal.


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Oysters...yuummmmm


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OfflinePoisonedV
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Re: Too many spores? [Re: oysterguy63]
    #7767887 - 12/16/07 11:20 PM (16 years, 1 month ago)

So the shit is starting to spread out more. Not enough heat, but it is still growing strong. Should I place the jar on an lizard-terrarium heater?


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Lazy...


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InvisibleCrake
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Re: Too many spores? [Re: PoisonedV]
    #7767917 - 12/16/07 11:31 PM (16 years, 1 month ago)

What temp. do you have them at? You know what, I think I'm going to go with the latest general consensus on this one... There have been a lot of threads lately stating that lower, as compared to higher temperatures are less likely to contaminate early on in the game. Mycelium will still grow with temperatures in the high 60s and low 70s and at these temperatures has a better chance at "out running" most other microbes within the confines of your jar.


--------------------
MANGO

Snowman wakes before dawn. He lies unmoving, listening to the tide coming in, wave after wave sloshing over the various barricades, wish-wash, wish-wash, the rhythm of heartbeat. He would so like to believe he is still asleep.


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OfflinePoisonedV
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Re: Too many spores? [Re: Crake]
    #7767955 - 12/16/07 11:46 PM (16 years, 1 month ago)

Inside temp. is about 68, and it is wrapped in a towel or 2


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Lazy...


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InvisibleGlacier Creek
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Re: Too many spores? [Re: PoisonedV]
    #7768056 - 12/17/07 12:33 AM (16 years, 1 month ago)

The only problem with shooting too much spore solution into a jar is all of the different substrains, meaning each pair of spores that met up to germinate, have to basicly "mate" to form one organism in order to fruit. Certain substrains are not compatable with one another, and will just refuse to conjoin with another.

Here is what RR had to say about it in one of my posts.


Quote:

RogerRabbit said:
Some growers have reported very fast 'colonization' with extremely full syringes. Healthy, fresh spores will begin to germinate within 20 minutes of being placed into grains or agar.

What hasn't ever been reported is nice flushes from very dark syringes. Any time you have thousands of substrains, at least a few will be non-compatible and won't merge into a single organism, which inhibits fruiting.

In addition, since no print is completely sterile, the more spores, the more contaminants that go along for the ride, thus often the contamination rates are higher.


RR




--------------------



Google "Earthly Info" to find my mushroom recipes. #1 baby.. yeah...

WARNING: All messages posted under this profile
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Offlineverminute
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Re: Too many spores? [Re: Glacier Creek]
    #7768552 - 12/17/07 08:37 AM (16 years, 1 month ago)

Yeah, I did the same thing, got about 5 cc into one jar. The rest of the jars about 1 - 1.5.

Note: don't inclulate jars at 7am without sleep, still drunk and stoned.

God damnit, what the hell was I thinking.


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InvisibleRoadkillM
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Re: Too many spores? [Re: Glacier Creek]
    #7768585 - 12/17/07 08:59 AM (16 years, 1 month ago)

Quote:

Glacier Creek said:

The only problem with shooting too much spore solution into a jar is all of the different substrains, meaning each pair of spores that met up to germinate, have to basicly "mate" to form one organism in order to fruit. Certain substrains are not compatable with one another, and will just refuse to conjoin with another.

Here is what RR had to say about it in one of my posts.


Quote:

RogerRabbit said:

Some growers have reported very fast 'colonization' with extremely full syringes. Healthy, fresh spores will begin to germinate within 20 minutes of being placed into grains or agar.

What hasn't ever been reported is nice flushes from very dark syringes. Any time you have thousands of substrains, at least a few will be non-compatible and won't merge into a single organism, which inhibits fruiting.

In addition, since no print is completely sterile, the more spores, the more contaminants that go along for the ride, thus often the contamination rates are higher.


RR








I think you are misunderstanding what RR meant in his post.

what RR is saying is that having too many spores or a dark syringe will cause problems.

he is not saying that using more spore solution is a problem.



In fact I have found that using 2 cc's of a (normal) spore syringe...

1/2 a cc in each pf jar hole(4 hole lid)...

rather than using 1 cc per pf tek jar...

with the pf teks Maximum fruiting formula will colonize faster and fruit better.



Maximum fruiting formula:
1/4 cup of brown rice powder
1/2 cup of vermiculite
60 cc's water


I can colonize a pf jar in 6 to 10 days!~

at 81 degrees F. in my incubator.

and I can colonize them even faster with an LC.




definition of a normal syringe : taking a 1/4" by 1/4" square from a spore print and making a syringe from that.

anything more is a waste of spores!~



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlineverminute
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Re: Too many spores? [Re: Roadkill]
    #7769637 - 12/17/07 01:26 PM (16 years, 1 month ago)

40cc equals to around 1/4 cup correct?


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InvisibleGlacier Creek
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Re: Too many spores? [Re: verminute]
    #7770567 - 12/17/07 05:23 PM (16 years, 1 month ago)

I didn't misunderstand anything. I was just pointing out the fact that more spores in a jar might mean faster colonization, but it could hurt the end result. I'm sure his 4cc is fine, but I have no idea what sort of syringe he used.

If it was an over packed syringe from say earthstongue.com and he used 4 cc he would have something to worry about. I know this, because I did almost the same thing, and the multispore flush was shit. The syringe was almost black it was so full of spores.

If this was similar to the one he used, I wanted to save him the trouble and move right to cloning, or pull a new print.

So I am not sure how I "misunderstood" what he was saying.


--------------------



Google "Earthly Info" to find my mushroom recipes. #1 baby.. yeah...

WARNING: All messages posted under this profile
are actually algorithmicly generated by an AI computer program.  No truth or actual events are being generated, and as a result cannot be investigated for thier validity.  (message 2345433)


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InvisibleBUDDHA_702
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Re: Too many spores? [Re: Glacier Creek]
    #7770579 - 12/17/07 05:27 PM (16 years, 1 month ago)

The spores wont hurt the extra water will. No way to fix it now just be more careful next time


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OfflinePoisonedV
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Re: Too many spores? [Re: BUDDHA_702]
    #7779951 - 12/19/07 11:19 PM (16 years, 1 month ago)

So the growth is stalling. It hasn't gotten any more mycelium in several days. I am worried.


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Offlinec0_hush
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Re: Too many spores? [Re: PoisonedV]
    #7779961 - 12/19/07 11:23 PM (16 years, 1 month ago)

Increase temp + check for proper gas exchange.


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OfflineNibin
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Re: Too many spores? [Re: BUDDHA_702]
    #7780388 - 12/20/07 03:50 AM (16 years, 1 month ago)

Quote:

BUDDHA_702 said:
The spores wont hurt the extra water will. No way to fix it now just be more careful next time




Too many spores will hurt, and probably more than 4ccs extra water. Read the quote from RR in this post.

Too many spores produce lots of competing substrains, some of which are not compatible. So instead of ending with a single mycelial network in your cake, you end up with a few, each fruiting independently and flushes will be smaller (as each individual network has access to less nutrients and water)

This said, there is no reason why this should be the case for the OP.


--------------------
Newcomers guide-----> For all things shroomy


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OfflinePoisonedV
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Re: Too many spores? [Re: c0_hush]
    #7783679 - 12/20/07 10:06 PM (16 years, 1 month ago)

thanks for the tip, I wrapped my heating blanket around it on 3 (out of ten) and made the air holes larger, I will see how it goes


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Lazy...


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InvisibleBUDDHA_702
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Re: Too many spores? [Re: Nibin]
    #7784322 - 12/21/07 02:07 AM (16 years, 1 month ago)

Quote:

Nibin said:
Quote:

BUDDHA_702 said:
The spores wont hurt the extra water will. No way to fix it now just be more careful next time




Too many spores will hurt, and probably more than 4ccs extra water. Read the quote from RR in this post.

Too many spores produce lots of competing substrains, some of which are not compatible. So instead of ending with a single mycelial network in your cake, you end up with a few, each fruiting independently and flushes will be smaller (as each individual network has access to less nutrients and water)

This said, there is no reason why this should be the case for the OP.




Good to know!
Thanks


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OfflineTheravadaDreams
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Re: Too many spores? [Re: BUDDHA_702]
    #7786188 - 12/21/07 04:34 PM (16 years, 1 month ago)

That is good to know. Any good strain isolation teks out there?


--------------------
"Believe nothing, no matter where you read it, or who said it, no matter if I have said it, unless it agrees with your own reason and your own common sense." -Siddhartha Guatama a.k.a Buddha.

MonsterMitch Mono-Tub.



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OfflineJOHNSTONE3737
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Re: Too many spores? [Re: PoisonedV]
    #7786231 - 12/21/07 04:45 PM (16 years, 1 month ago)

WELL, IT DEPENDS ON THE MOISTURE CONTENT OF THAT PARTICULAR JAR.  IF THE JAR WAS SLIGHTLY ON THE DRY SIDE, THEN YOU MAY NOT HAVE ANYTHING TO WORRY ABOUT.  IF IT'S TOO "WATER LOGGED" NOW, THEN IT MAY JUST TAKE LONGER TO COLONIZE.  OF COURSE THE LONGER THE JAR IS IN THE INCUBATION PERIOD, THE BETTER CHANCE IT HAS OF ACQUIRING AN INFECTION FROM A COMPETING MICRO-ORGANISM.  YOU WANT YOUR JARS TO COLONIZE WITH MYCELIUM AS QUICKLY AS POSSIBLE.  HOPEFULLY YOU IMPLEMENTED STERILE TECHNIQUES TO THE BEST OF YOUR ABILITY AND THEREFORE WON'T HAVE MUCH TO WORRY ABOUT  :smile:


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OfflineNibin
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Re: Too many spores? [Re: JOHNSTONE3737]
    #7786233 - 12/21/07 04:46 PM (16 years, 1 month ago)

Quote:

JOHNSTONE3737 said:
WELL, IT DEPENDS ON THE MOISTURE CONTENT OF THAT PARTICULAR JAR.  IF THE JAR WAS SLIGHTLY ON THE DRY SIDE, THEN YOU MAY NOT HAVE ANYTHING TO WORRY ABOUT.  IF IT'S TOO "WATER LOGGED" NOW, THEN IT MAY JUST TAKE LONGER TO COLONIZE.  OF COURSE THE LONGER THE JAR IS IN THE INCUBATION PERIOD, THE BETTER CHANCE IT HAS OF ACQUIRING AN INFECTION FROM A COMPETING MICRO-ORGANISM.  YOU WANT YOUR JARS TO COLONIZE WITH MYCELIUM AS QUICKLY AS POSSIBLE.  HOPEFULLY YOU IMPLEMENTED STERILE TECHNIQUES TO THE BEST OF YOUR ABILITY AND THEREFORE WON'T HAVE MUCH TO WORRY ABOUT  :smile:




Arg, CAPS!!!!!!

EDIT: Even your username is in caps. You got a broken keyboard?


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