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Invisibleflavoraid
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post reguarding tyvek , LC and cakes.
    #7761658 - 12/15/07 12:32 PM (16 years, 1 month ago)

Is it bad if tyvek gets wet or is that just polyfil?

In my liquid culture jar I want to have 3 layers of coffee filters as a dust cover held down with a rubber band, tyvek layer x 2 inoc hole covered with microspore tape and another breather whole in the lid, then the lid of course the jar ring will hold down the tyvek.

Now for cakes should I even bother using tyvek / microspore tape?
If so can anyone give me an idea of what I should do? Usually cakes are inoculated at four points the LC doesn't matter as it's liquid so the setup would be different.


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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Invisibleflavoraid
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Re: post reguarding tyvek , LC and cakes. [Re: flavoraid]
    #7761672 - 12/15/07 12:36 PM (16 years, 1 month ago)

I should also mention I looked into using tyvek for cakes because I believe someone mentioned not needing the vermiculite layer in jars


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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Offlineveda_sticks
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Re: post reguarding tyvek , LC and cakes. [Re: flavoraid]
    #7761828 - 12/15/07 01:37 PM (16 years, 1 month ago)

Thats overkill, the tyvek itself is plenty. If the tyvek gets wet then contams can wick threw it.

Micropore tape over the inoc holes with the tyvek is plenty.

Yes its true, if you have a tyvek filter then you dont need the verm layer.


--------------------

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Invisibleflavoraid
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Re: post reguarding tyvek , LC and cakes. [Re: veda_sticks]
    #7763111 - 12/15/07 08:16 PM (16 years, 1 month ago)

so with a liquid culture I shouldn't fill it up very high up?

should I bother with tyvek on my cakes or should I just use microspore tape?


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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OfflineNibin
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Re: post reguarding tyvek , LC and cakes. [Re: flavoraid]
    #7764334 - 12/16/07 12:47 AM (16 years, 1 month ago)

micropore over the inoculation holes is a valid substitute for the dry verm layer in PF cakes


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OfflinePentahedral
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Re: post reguarding tyvek , LC and cakes. [Re: Nibin]
    #7764520 - 12/16/07 01:28 AM (16 years, 1 month ago)

What about if the tyvek gets wet in PC'ing, when I took the foil off to inoculate it was a bit wet, is that going to be a problem?


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Invisibleflavoraid
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Re: post reguarding tyvek , LC and cakes. [Re: Pentahedral]
    #7766115 - 12/16/07 03:53 PM (16 years, 1 month ago)

Pentahdral did you cover your jars with foil? Spores should come on monday (tomorrow) and I wanna get busy! :laugh:


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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Invisibleflavoraid
now with twicethe ketamine andopiates!
Male

Registered: 12/05/07
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Re: post reguarding tyvek , LC and cakes. [Re: flavoraid]
    #7768999 - 12/17/07 11:13 AM (16 years, 1 month ago)

bump!

I hear filter discs you buy won't wick contams

also is it possible for my stirrer to be too fast? People say you can beat the mycelium to death well one person did but it still brings concern...

INFO plz :laugh:


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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OfflineRogerRabbitM
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Re: post reguarding tyvek , LC and cakes. [Re: flavoraid]
    #7769028 - 12/17/07 11:20 AM (16 years, 1 month ago)

Filter disks will normally get damp during sterilization. Remove the foil covering when you remove from the pressure cooker so the tyvek or other material can dry out.

If a commercially purchased synthetic filter disk gets wet, as with any other filter material, bacterial contaminants can colonize right through the filter to reach your media below.

The stirrer does two things with a properly made liquid culture. First it keeps the liquid aerated. Second, it prevents the fragmeted mycelium from re-knitting together. You want hundreds, or even thousands of tiny fragments of mycelium, not a single organism which is hard to draw into a syringe.
RR


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Invisibletahoe
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Re: post reguarding tyvek , LC and cakes. [Re: RogerRabbit]
    #7769359 - 12/17/07 12:24 PM (16 years, 1 month ago)

^^^^^^^^^^

tyvek or filter disc have no use in lc's
If it gets wet you are screwed. Polyfill is better for lc's. The trick is not getting it too wet. Some liquid will hit it but it hangs 1/2inch beloiw the lid so it still will not contam.

Just do not get out of control when you swirrel your jars.


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibleflavoraid
now with twicethe ketamine andopiates!
Male

Registered: 12/05/07
Posts: 1,678
Re: post reguarding tyvek , LC and cakes. [Re: tahoe]
    #7769631 - 12/17/07 01:25 PM (16 years, 1 month ago)

how about microspore tape? polyfill just bothers me

if microspore does the trick for ge i could just inject and leave it on my stirrer (have a resistor so theres no vortex) i could even make 2 holes 1 solely for breathing and 1 for the inoc

does microspore wick contams?


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coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


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Invisibleflavoraid
now with twicethe ketamine andopiates!
Male

Registered: 12/05/07
Posts: 1,678
Re: post reguarding tyvek , LC and cakes. [Re: flavoraid]
    #7773616 - 12/18/07 12:22 PM (16 years, 1 month ago)

i think im going to do the agar method of the tubes stuffed with polyfil seems like such a good idea

heat resistant tubing siliconed onto a jar lid, tubes get stuffed with polyfil and you clamp them off when you want to shake them or w.e and u can tilt it sideways with the polyfil removed from one tube and the other clamped off to extract LC

looks neat as hella!


--------------------
coda said:
imachavel, Man you really need to do some reading, the amount of bullshit you put into almost every single one of your posts is absolutely astounding.


Extras: Filter Print Post Top
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