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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,423
Last seen: 18 hours, 54 minutes
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Spore microscopy photos
#7760612 - 12/14/07 11:31 PM (16 years, 6 months ago) |
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I got some spores in the mail from BlimeyGrimey and took some pictures of them.
Condensation on a petri dish cover:

The rest of these pics are at 1000x. No zooming or photo editing was done so the spore sizes can be compared.
Psilocybe stuntzii. These have small subrhomboid spores which look very similar to P. ovoideocystidiata or P. subaeruginascens spores:

Another shot of P. stuntzii spores:

This print was labeled Psilocybe cyanofibrillosa specimen C - Seattle, WA. The spores are much larger than the spores of P. cyanofibrillosa, they look about the same size as P. cyanescens. The mushroom that made these spores is probably in the P. cyanofriscosa complex.

P. cyanescens spores, Seattle, WA:

G. luteofolius spores. I compared them with G. luteofolius spores from my collection and they matched. These spores are finely warted, as you can see in a few of the spores which are at the right height to be focused on the outer layer.
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ILLegal
Stranger
Registered: 11/29/07
Posts: 11
Last seen: 16 years, 29 days
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Quote:
Alan Rockefeller said:

Is it just me or does the picture seem to move?
Oh and nice by the way.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,423
Last seen: 18 hours, 54 minutes
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Re: Spore microscopy photos [Re: ILLegal]
#7760883 - 12/15/07 03:00 AM (16 years, 6 months ago) |
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Here is a gill edge from a Gymnopilus I found in a log in GGP:

Psilocybe atlantis spores:

More Psilocybe atlantis spores:
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
Posts: 3,807
Loc: Puget Sound
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Quote:
Alan Rockefeller said: This print was labeled Psilocybe cyanofibrillosa specimen C - Seattle, WA. The spores are much larger than the spores of P. cyanofibrillosa, they look about the same size as P. cyanescens. The mushroom that made these spores is probably in the P. cyanofriscosa complex.

Here's a pic of the cyano's.

You can see specimen "C" to the right. It was hidden under weeds next to specimen "B".
They do not resemble cyanescens while in their immature or mature phases. Here's a pic of the smaller ones I found.

I believe I have found the same species that SporeWorks has listed as cyanofibrillosa, as Workman has said that their collection is infact NOT cyanofibrillosa. He has been sent dried gill samples to further identify this species.
Thanks Rockefeller! I knew these cyanofubs seemed a bit different than the macroscopic textbook description of fibs. Now we have microscopic proof also.
Another thanks for verifying my luetofolius find. I was unsure as to whether they were luetofolius or aeruginosus.
Sorry for the VERY VERY small stunztii print but it was all I had left other than my last decent print. Glad there was enough spores for you to work with.
Did the stuntzii spores match up to the textbook description of the spores?
Pics of the stuntzii I found last year and this year.
This year's pic:

Last year's pic (from the same patch):

Thanks again Rockefeller! We need more people like you with camera's for their scopes. I hope to get one soon!
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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undergrounder
fluffy bunny



Registered: 11/10/06
Posts: 1,394
Loc: Sydney
Last seen: 2 years, 18 days
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Re: Spore microscopy photos [Re: BlimeyGrimey]
#7760979 - 12/15/07 05:17 AM (16 years, 6 months ago) |
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Quote:
BlimeyGrimey said: Here's a pic of the cyano's.

You can see specimen "C" to the right. It was hidden under weeds next to specimen "B".
They do not resemble cyanescens while in their immature or mature phases. Here's a pic of the smaller ones I found.

Those look a lot like the colder growing Ps. subaeruginosa we get here. They don't seem to open up fully, and they seem more chunky than their UFO or wavy-shaped cousins.
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RIP Bigger and bolder and rougher and tougher in other words sucka there is no other...
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
Posts: 3,807
Loc: Puget Sound
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I showed those mushrooms to a friend and they said they looked like Ps. subaeruginosa. When I told them where they had been picked he then said they were probably cyanofibrillosa.
Alan, do you have a description of Ps. subaeruginosa to compare those cyanofib spores to?
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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sui
Lucky Shot Inc. š



Registered: 08/20/04
Posts: 33,084
Loc: Cali, Contra Costa Co.
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Re: Spore microscopy photos [Re: BlimeyGrimey]
#7761203 - 12/15/07 09:02 AM (16 years, 6 months ago) |
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fucking NICE Alan!
-------------------- "There is never a wrong note, bend it." Jimi Hendrix
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,423
Last seen: 18 hours, 54 minutes
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Re: Spore microscopy photos [Re: BlimeyGrimey]
#7761617 - 12/15/07 12:20 PM (16 years, 6 months ago) |
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> I believe I have found the same species that SporeWorks has listed as cyanofibrillosa, as Workman has said that their collection is infact NOT cyanofibrillosa. He has been sent dried gill samples to further identify this species.
Yes, good chance it is that one.
> Did the stuntzii spores match up to the textbook description of the spores?
Yes, they do.
> Another thanks for verifying my luetofolius find. I was unsure as to whether they were luetofolius or aeruginosus.
The spores for those two species are identical.
> Those look a lot like the colder growing Ps. subaeruginosa we get here. They don't seem to open up fully, and they seem more chunky than their UFO or wavy-shaped cousins.
> I showed those mushrooms to a friend and they said they looked like Ps. subaeruginosa. When I told them where they had been picked he then said they were probably cyanofibrillosa.
> Alan, do you have a description of Ps. subaeruginosa to compare those cyanofib spores to?
P. cyanescens spores are 9-12 x 5 - 8, elongate-ellipsoid while P. subaeruginosa has spores that are 13-15 x 6.6 - 7.6 microns and have a subellipsoid shape.
Just based on the spores it looks like a good match for P. subaeruginosa, they are slightly larger and differently shaped than the P. cyanescens spores. It would be interesting to see what the pleurocystidia and cheilocystidia look like.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,423
Last seen: 18 hours, 54 minutes
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Here are two pictures of P. subaeruginascens spores, not to be confused with P. subaeruginosa.
These are from a GGP collection. The spores a small and subrhomboid, very similar to P. stuntzii spores.

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2859558484
Growery is Better



Registered: 01/10/06
Posts: 8,752
Last seen: 3 years, 9 months
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wow,alan you are fucking cool. keep it up.
this is like a free mycology class with a badass professor that smokes blueberry chron with you.
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never_2_high
Stoner


Registered: 03/05/06
Posts: 218
Last seen: 13 years, 6 months
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Que lindas fotos Alan! Me encantan.
Edited by never_2_high (12/15/07 03:05 PM)
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casgoodie
weedwright


Registered: 10/31/06
Posts: 770
Loc: terra
Last seen: 10 years, 8 months
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Re: Spore microscopy photos [Re: never_2_high]
#7761981 - 12/15/07 02:23 PM (16 years, 6 months ago) |
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thanks for sharing, much appreciated.
-------------------- TRAPPED IN LINGUISTIC CONCEPTS
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GGreatOne234
Stranger
Registered: 12/23/99
Posts: 8,946
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Re: Spore microscopy photos [Re: casgoodie]
#7762443 - 12/15/07 04:39 PM (16 years, 6 months ago) |
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Beautiful pictures Alan.
You're a mad-scientist hehehe:)
I honestly do not know too much about microscopic features of mushrooms yet. I do not have a microscope (yet), but that would be the end of the line officially making me a mycological wack-job. It'd be too intense for me lol. After seeing your cool images and reading your microscopy-related mushroom-definitions (of which I am not very familiar with), it makes me want to get the "How to Identify Mushrooms to Genus" book involving microscopy. Is that the book from where you started to learn all this? It seems like the only book I know of that deals with the topic in depth.
I'm still trying to digest "How to Identfy Mushrooms to Genus" parts one and two..
Hey great stuff man, good work, thanks for sharing.
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Subbedhunter420
Solitary Hunter



Registered: 12/30/06
Posts: 1,501
Loc: LA/Ventura County
Last seen: 9 years, 14 days
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Re: Spore microscopy photos [Re: GGreatOne234]
#7762527 - 12/15/07 05:05 PM (16 years, 6 months ago) |
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Those are some amazing pics alan! youve inspired me to buy my own microscope as soon as i can to start working on the panaeolus down here.
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cactu
culture and magic


Registered: 03/06/06
Posts: 3,913
Loc: mexicoelcentrodelconocimi...
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HOLA SEĆOR ALANROCKEFELLER me dirigo ante usted de la manera mas cordial , para ver la posibilidad de enviarle un gran saludo , de agradecerle el trabajo tan duro y constante que realiza para todos nosotros, me da mucho gusto observas sus fotos, me llevan a lugares desconocidos de mi mente y me conectan directamente con el destino, yo soy un poeta seƱor , y sus acciones me inspiran a crear las lineas mas intricadas en los misterios del bosque , pero en esta ocasion quiero preguntarle si seria oposible mandarle cierto material para mantener la esperanza y la concentracion fijada en un solo punto , me gustaria saber por que no hay basidios en estas fotos , pero a lo mejor no conto con dicho material , por lo que prontamente me agradaria mandarle un material de trabajo nuevo , me despido de usted deseandole que sus sueƱos se hagan realidad. para usted mis mejores vibraciones .......
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  cuando una rafaga del pensamiento nos pasa al lado se puede sentir que valio la pena haber vivido, y cuando ese pensamiento se convierte en sueƱo no paramos de soƱar hasta realizarlo
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GGreatOne234
Stranger
Registered: 12/23/99
Posts: 8,946
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Re: Spore microscopy photos [Re: cactu]
#7762751 - 12/15/07 06:07 PM (16 years, 6 months ago) |
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ehhhh-cactu,, I was too stoned every morning during first-period Spanish class in high school to make heads or tails of all-that.. took two years of Spanish, always first period.. and believe me when I say that constantly smoking bongs at home while trying to memorize words in a different language doesn't work out so well for me.
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Rekkuzo
Lord EmperorButto



Registered: 10/09/07
Posts: 246
Loc: Nor'East
Last seen: 11 years, 4 months
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Re: Spore microscopy photos [Re: cactu]
#7763001 - 12/15/07 07:36 PM (16 years, 6 months ago) |
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Quote:
cactu said: HOLA SEĆOR ALANROCKEFELLER me dirigo ante usted de la manera mas cordial , para ver la posibilidad de enviarle un gran saludo , de agradecerle el trabajo tan duro y constante que realiza para todos nosotros, me da mucho gusto observas sus fotos, me llevan a lugares desconocidos de mi mente y me conectan directamente con el destino, yo soy un poeta seƱor , y sus acciones me inspiran a crear las lineas mas intricadas en los misterios del bosque , pero en esta ocasion quiero preguntarle si seria oposible mandarle cierto material para mantener la esperanza y la concentracion fijada en un solo punto , me gustaria saber por que no hay basidios en estas fotos , pero a lo mejor no conto con dicho material , por lo que prontamente me agradaria mandarle un material de trabajo nuevo , me despido de usted deseandole que sus sueƱos se hagan realidad. para usted mis mejores vibraciones .......
Translation: Hello, Mr. Alan Rockefeller. I bring myself before you in the most cordial manner, to see the possibility of sending you a grand greeting, to thank you for the constant and hard work that you do for us, I very much enjoy looking at your pictures, they take me to unknown places within my mind and connect me directly to destiny, I am a poet sir, and your actions inspire me to create more intricate lines on the mysteries of the forest, but in this occasion I want to ask if it would be possible(typo?) to send you certain material to keep your hope and and concentration fixated on one single point, I would like to know why there aren't basidia in those photos, but on the other hand maybe I shouldn't count on said material, for I would promptly like to send you another subject to study, I bid you farewell now, wishing that your dreams become reality. All my best vibrations.
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GGreatOne234
Stranger
Registered: 12/23/99
Posts: 8,946
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Re: Spore microscopy photos [Re: Rekkuzo]
#7763058 - 12/15/07 07:57 PM (16 years, 6 months ago) |
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OOooo, la-dee-freakin-doo-da!
How many years did you study spanish to translate like that?
Nicely done i must say
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cactu
culture and magic


Registered: 03/06/06
Posts: 3,913
Loc: mexicoelcentrodelconocimi...
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Re: Spore microscopy photos [Re: GGreatOne234]
#7763066 - 12/15/07 08:01 PM (16 years, 6 months ago) |
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hola GG234 vas a tener que repasar tus lecciones de espaƱol una ves mas , he comprobado en mis muchas formas de aprender ingles que fumar incrementa la atencion subjetiva y me ayuda a expresarme , ademas de ser una herramienta basica para conectar con los espiritus de los hongos cuando estoy en la busqueda de ellos , sabes que estoy haciendo esto para que vallas practicando y no sea dificil decir ME ENCANTA MEXICO , OTRO MAS POR FAVOR, LA VIDA ES VIDA Y CON HONGOS SUPER VIDA ......COSAS POR EL ESTILO . WELL ALL MY BEST VIBRATIONS FROM THE OTHER SIDE ..........
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  cuando una rafaga del pensamiento nos pasa al lado se puede sentir que valio la pena haber vivido, y cuando ese pensamiento se convierte en sueƱo no paramos de soƱar hasta realizarlo
Edited by cactu (12/15/07 08:12 PM)
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,423
Last seen: 18 hours, 54 minutes
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Re: Spore microscopy photos [Re: GGreatOne234]
#7763079 - 12/15/07 08:08 PM (16 years, 6 months ago) |
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Hypholoma sublateritium, 1000x, zoomed out:

Hypholoma sublateritium, 1000x, zoomed in:

Gymnopilus luteofolius, 1000x, zoomed in:

G. luteofolius, 1000s, zoomed in:

Psilocybe zapotecorum from Colima, Mexico:

P. zapotecorum Colima:
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