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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Spore microscopy photos
#7760612 - 12/14/07 11:31 PM (16 years, 1 month ago) |
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I got some spores in the mail from BlimeyGrimey and took some pictures of them.
Condensation on a petri dish cover:

The rest of these pics are at 1000x. No zooming or photo editing was done so the spore sizes can be compared.
Psilocybe stuntzii. These have small subrhomboid spores which look very similar to P. ovoideocystidiata or P. subaeruginascens spores:

Another shot of P. stuntzii spores:

This print was labeled Psilocybe cyanofibrillosa specimen C - Seattle, WA. The spores are much larger than the spores of P. cyanofibrillosa, they look about the same size as P. cyanescens. The mushroom that made these spores is probably in the P. cyanofriscosa complex.

P. cyanescens spores, Seattle, WA:

G. luteofolius spores. I compared them with G. luteofolius spores from my collection and they matched. These spores are finely warted, as you can see in a few of the spores which are at the right height to be focused on the outer layer.
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ILLegal
Stranger
Registered: 11/29/07
Posts: 11
Last seen: 15 years, 7 months
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Quote:
Alan Rockefeller said:

Is it just me or does the picture seem to move?
Oh and nice by the way.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: ILLegal]
#7760883 - 12/15/07 03:00 AM (16 years, 1 month ago) |
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Here is a gill edge from a Gymnopilus I found in a log in GGP:

Psilocybe atlantis spores:

More Psilocybe atlantis spores:
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
Posts: 3,788
Loc: Puget Sound
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Quote:
Alan Rockefeller said: This print was labeled Psilocybe cyanofibrillosa specimen C - Seattle, WA. The spores are much larger than the spores of P. cyanofibrillosa, they look about the same size as P. cyanescens. The mushroom that made these spores is probably in the P. cyanofriscosa complex.

Here's a pic of the cyano's.

You can see specimen "C" to the right. It was hidden under weeds next to specimen "B".
They do not resemble cyanescens while in their immature or mature phases. Here's a pic of the smaller ones I found.

I believe I have found the same species that SporeWorks has listed as cyanofibrillosa, as Workman has said that their collection is infact NOT cyanofibrillosa. He has been sent dried gill samples to further identify this species.
Thanks Rockefeller! I knew these cyanofubs seemed a bit different than the macroscopic textbook description of fibs. Now we have microscopic proof also.
Another thanks for verifying my luetofolius find. I was unsure as to whether they were luetofolius or aeruginosus.
Sorry for the VERY VERY small stunztii print but it was all I had left other than my last decent print. Glad there was enough spores for you to work with.
Did the stuntzii spores match up to the textbook description of the spores?
Pics of the stuntzii I found last year and this year.
This year's pic:

Last year's pic (from the same patch):

Thanks again Rockefeller! We need more people like you with camera's for their scopes. I hope to get one soon!
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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undergrounder
fluffy bunny



Registered: 11/10/06
Posts: 1,394
Loc: Sydney
Last seen: 1 year, 7 months
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Quote:
BlimeyGrimey said: Here's a pic of the cyano's.

You can see specimen "C" to the right. It was hidden under weeds next to specimen "B".
They do not resemble cyanescens while in their immature or mature phases. Here's a pic of the smaller ones I found.

Those look a lot like the colder growing Ps. subaeruginosa we get here. They don't seem to open up fully, and they seem more chunky than their UFO or wavy-shaped cousins.
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RIP Bigger and bolder and rougher and tougher in other words sucka there is no other...
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
Posts: 3,788
Loc: Puget Sound
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I showed those mushrooms to a friend and they said they looked like Ps. subaeruginosa. When I told them where they had been picked he then said they were probably cyanofibrillosa.
Alan, do you have a description of Ps. subaeruginosa to compare those cyanofib spores to?
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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sui
I love you.



Registered: 08/20/04
Posts: 31,853
Loc: Cali, Contra Costa Co.
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fucking NICE Alan!
-------------------- "There is never a wrong note, bend it." Jimi Hendrix
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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> I believe I have found the same species that SporeWorks has listed as cyanofibrillosa, as Workman has said that their collection is infact NOT cyanofibrillosa. He has been sent dried gill samples to further identify this species.
Yes, good chance it is that one.
> Did the stuntzii spores match up to the textbook description of the spores?
Yes, they do.
> Another thanks for verifying my luetofolius find. I was unsure as to whether they were luetofolius or aeruginosus.
The spores for those two species are identical.
> Those look a lot like the colder growing Ps. subaeruginosa we get here. They don't seem to open up fully, and they seem more chunky than their UFO or wavy-shaped cousins.
> I showed those mushrooms to a friend and they said they looked like Ps. subaeruginosa. When I told them where they had been picked he then said they were probably cyanofibrillosa.
> Alan, do you have a description of Ps. subaeruginosa to compare those cyanofib spores to?
P. cyanescens spores are 9-12 x 5 - 8, elongate-ellipsoid while P. subaeruginosa has spores that are 13-15 x 6.6 - 7.6 microns and have a subellipsoid shape.
Just based on the spores it looks like a good match for P. subaeruginosa, they are slightly larger and differently shaped than the P. cyanescens spores. It would be interesting to see what the pleurocystidia and cheilocystidia look like.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Here are two pictures of P. subaeruginascens spores, not to be confused with P. subaeruginosa.
These are from a GGP collection. The spores a small and subrhomboid, very similar to P. stuntzii spores.

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2859558484
Growery is Better



Registered: 01/10/06
Posts: 8,752
Last seen: 3 years, 4 months
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wow,alan you are fucking cool. keep it up.
this is like a free mycology class with a badass professor that smokes blueberry chron with you.
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never_2_high
Stoner


Registered: 03/05/06
Posts: 218
Last seen: 13 years, 1 month
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Que lindas fotos Alan! Me encantan.
Edited by never_2_high (12/15/07 03:05 PM)
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casgoodie
weedwright


Registered: 10/31/06
Posts: 770
Loc: terra
Last seen: 10 years, 3 months
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thanks for sharing, much appreciated.
-------------------- TRAPPED IN LINGUISTIC CONCEPTS
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GGreatOne234
Stranger
Registered: 12/23/99
Posts: 8,946
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Re: Spore microscopy photos [Re: casgoodie]
#7762443 - 12/15/07 04:39 PM (16 years, 1 month ago) |
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Beautiful pictures Alan.
You're a mad-scientist hehehe:)
I honestly do not know too much about microscopic features of mushrooms yet. I do not have a microscope (yet), but that would be the end of the line officially making me a mycological wack-job. It'd be too intense for me lol. After seeing your cool images and reading your microscopy-related mushroom-definitions (of which I am not very familiar with), it makes me want to get the "How to Identify Mushrooms to Genus" book involving microscopy. Is that the book from where you started to learn all this? It seems like the only book I know of that deals with the topic in depth.
I'm still trying to digest "How to Identfy Mushrooms to Genus" parts one and two..
Hey great stuff man, good work, thanks for sharing.
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Subbedhunter420
Solitary Hunter



Registered: 12/30/06
Posts: 1,501
Loc: LA/Ventura County
Last seen: 8 years, 7 months
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Those are some amazing pics alan! youve inspired me to buy my own microscope as soon as i can to start working on the panaeolus down here.
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cactu
culture and magic


Registered: 03/06/06
Posts: 3,913
Loc: mexicoelcentrodelconocimi...
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HOLA SEÑOR ALANROCKEFELLER me dirigo ante usted de la manera mas cordial , para ver la posibilidad de enviarle un gran saludo , de agradecerle el trabajo tan duro y constante que realiza para todos nosotros, me da mucho gusto observas sus fotos, me llevan a lugares desconocidos de mi mente y me conectan directamente con el destino, yo soy un poeta señor , y sus acciones me inspiran a crear las lineas mas intricadas en los misterios del bosque , pero en esta ocasion quiero preguntarle si seria oposible mandarle cierto material para mantener la esperanza y la concentracion fijada en un solo punto , me gustaria saber por que no hay basidios en estas fotos , pero a lo mejor no conto con dicho material , por lo que prontamente me agradaria mandarle un material de trabajo nuevo , me despido de usted deseandole que sus sueños se hagan realidad. para usted mis mejores vibraciones .......
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  cuando una rafaga del pensamiento nos pasa al lado se puede sentir que valio la pena haber vivido, y cuando ese pensamiento se convierte en sueño no paramos de soñar hasta realizarlo
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GGreatOne234
Stranger
Registered: 12/23/99
Posts: 8,946
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Re: Spore microscopy photos [Re: cactu]
#7762751 - 12/15/07 06:07 PM (16 years, 1 month ago) |
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ehhhh-cactu,, I was too stoned every morning during first-period Spanish class in high school to make heads or tails of all-that.. took two years of Spanish, always first period.. and believe me when I say that constantly smoking bongs at home while trying to memorize words in a different language doesn't work out so well for me.
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Rekkuzo
Lord EmperorButto



Registered: 10/09/07
Posts: 246
Loc: Nor'East
Last seen: 10 years, 11 months
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Re: Spore microscopy photos [Re: cactu]
#7763001 - 12/15/07 07:36 PM (16 years, 1 month ago) |
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Quote:
cactu said: HOLA SEÑOR ALANROCKEFELLER me dirigo ante usted de la manera mas cordial , para ver la posibilidad de enviarle un gran saludo , de agradecerle el trabajo tan duro y constante que realiza para todos nosotros, me da mucho gusto observas sus fotos, me llevan a lugares desconocidos de mi mente y me conectan directamente con el destino, yo soy un poeta señor , y sus acciones me inspiran a crear las lineas mas intricadas en los misterios del bosque , pero en esta ocasion quiero preguntarle si seria oposible mandarle cierto material para mantener la esperanza y la concentracion fijada en un solo punto , me gustaria saber por que no hay basidios en estas fotos , pero a lo mejor no conto con dicho material , por lo que prontamente me agradaria mandarle un material de trabajo nuevo , me despido de usted deseandole que sus sueños se hagan realidad. para usted mis mejores vibraciones .......
Translation: Hello, Mr. Alan Rockefeller. I bring myself before you in the most cordial manner, to see the possibility of sending you a grand greeting, to thank you for the constant and hard work that you do for us, I very much enjoy looking at your pictures, they take me to unknown places within my mind and connect me directly to destiny, I am a poet sir, and your actions inspire me to create more intricate lines on the mysteries of the forest, but in this occasion I want to ask if it would be possible(typo?) to send you certain material to keep your hope and and concentration fixated on one single point, I would like to know why there aren't basidia in those photos, but on the other hand maybe I shouldn't count on said material, for I would promptly like to send you another subject to study, I bid you farewell now, wishing that your dreams become reality. All my best vibrations.
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GGreatOne234
Stranger
Registered: 12/23/99
Posts: 8,946
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Re: Spore microscopy photos [Re: Rekkuzo]
#7763058 - 12/15/07 07:57 PM (16 years, 1 month ago) |
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OOooo, la-dee-freakin-doo-da!
How many years did you study spanish to translate like that?
Nicely done i must say
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cactu
culture and magic


Registered: 03/06/06
Posts: 3,913
Loc: mexicoelcentrodelconocimi...
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hola GG234 vas a tener que repasar tus lecciones de español una ves mas , he comprobado en mis muchas formas de aprender ingles que fumar incrementa la atencion subjetiva y me ayuda a expresarme , ademas de ser una herramienta basica para conectar con los espiritus de los hongos cuando estoy en la busqueda de ellos , sabes que estoy haciendo esto para que vallas practicando y no sea dificil decir ME ENCANTA MEXICO , OTRO MAS POR FAVOR, LA VIDA ES VIDA Y CON HONGOS SUPER VIDA ......COSAS POR EL ESTILO . WELL ALL MY BEST VIBRATIONS FROM THE OTHER SIDE ..........
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  cuando una rafaga del pensamiento nos pasa al lado se puede sentir que valio la pena haber vivido, y cuando ese pensamiento se convierte en sueño no paramos de soñar hasta realizarlo
Edited by cactu (12/15/07 08:12 PM)
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Hypholoma sublateritium, 1000x, zoomed out:

Hypholoma sublateritium, 1000x, zoomed in:

Gymnopilus luteofolius, 1000x, zoomed in:

G. luteofolius, 1000s, zoomed in:

Psilocybe zapotecorum from Colima, Mexico:

P. zapotecorum Colima:
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Rekkuzo
Lord EmperorButto



Registered: 10/09/07
Posts: 246
Loc: Nor'East
Last seen: 10 years, 11 months
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Lol, born in Puerto Rico, no study required.
Hola cactu, me encantan tus fotos.
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
Posts: 3,788
Loc: Puget Sound
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Babelfish translates it in less then 2 seconds .....
Rekkuzo: Nice to see another puerto rican on the forums! Unfortunately I only lived there for 6 months as a baby then moved to the mainland.
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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Rekkuzo
Lord EmperorButto



Registered: 10/09/07
Posts: 246
Loc: Nor'East
Last seen: 10 years, 11 months
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I moved shortly after my 7th birthday. I kept all my Spanish skillz, but my vocabulary is pretty limited, though speaking with my parents constantly helps.
Lol, I just checked what babelfish would come up with, and it's completely bastardized.
Also, why do the zapotecorum spores look all "dirty"?
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cactu
culture and magic


Registered: 03/06/06
Posts: 3,913
Loc: mexicoelcentrodelconocimi...
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hola rekkuzo , gracias por lo de las fotos y la traduccion para el señor alan rocketfeller, ahh, el español es tan bonito y poetico mucho mas que el ingles , pero bueno hay que ponerle salsa al foro deves encuando no ., saludos my best vibration for you i bet spores look dirty due to alan travel method
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  cuando una rafaga del pensamiento nos pasa al lado se puede sentir que valio la pena haber vivido, y cuando ese pensamiento se convierte en sueño no paramos de soñar hasta realizarlo
Edited by cactu (12/15/07 08:25 PM)
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: cactu]
#7764852 - 12/16/07 10:24 AM (16 years, 1 month ago) |
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> wow,alan you are fucking cool. keep it up. > this is like a free mycology class with a badass professor that smokes blueberry chron with you.
Thanks, that is exactly what I was going for.
> You're a mad-scientist hehehe:)
Haha you should see my garage.
> I do not have a microscope (yet), but that would be the end of the line officially making me a mycological wack-job.
Sounds like a good reason to get one.
Plus, the chicks really dig them.
> it makes me want to get the "How to Identify Mushrooms to Genus" book involving microscopy. Is that the book from where you started to learn all this?
No, but I plan to get those next.
> It seems like the only book I know of that deals with the topic in depth.
I am reading The Cytology and Plectology of the Hymenomycetes.
> Those are some amazing pics alan! youve inspired me to buy my own microscope as soon as i can to start working on the panaeolus down here.
I have this one, its great. I have a camera mounted to its third eye now, its fun to sit back and pan around the slide, watching the spores fly across the LCD screen in the camera.
> i bet spores look dirty due to alan travel method
The print is really light, Its hard to get a good sample on to the slide without scraping up some paper too.
Bluing Pluteus species, Curecat collection, CA

Bluing Pluteus species, Curecat collection, CA

Psilocybe cubensis, Waxingren lawn clippings collection

Psilocybe cubensis, Waxingren lawn clippings collection

Panaeolus subbalteatus, subbedhunter420 collection

Panaeolus subbalteatus, subbedhunter420 collection

Panaeolus subbalteatus, subbedhunter420 collection

Psilocybe ovoideocystidiata - Belmont - Shroomydan collection

Psilocybe ovoideocystidiata - Belmont - Shroomydan collection

Psilocybe ovoideocystidiata - Bethany - Shroomydan collection

Psilocybe ovoideocystidiata - Bethany - Shroomydan collection
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cactu
culture and magic


Registered: 03/06/06
Posts: 3,913
Loc: mexicoelcentrodelconocimi...
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you are omnipresent alan ,  shroomery have the best animations are you gonna past the line with such a beautiful introduction to another world ,.. all my best vibrations, do you have a pcr stickiest devise, to perform genome exchange in interest into mutated to the next genus. or at least initiate a symbiotic relationship even in intracellular linking , so far i have only try the old methods . but will interest to see if there is Good machine to id all mushrooms with a projection to the future and back and give you all details in present time.. you see i´m getting crazy , yes is the dry season symptoms affect all my brains i have 2 , i guess is the lack of moisture, or to little excitement, but the cyber world feed me........
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  cuando una rafaga del pensamiento nos pasa al lado se puede sentir que valio la pena haber vivido, y cuando ese pensamiento se convierte en sueño no paramos de soñar hasta realizarlo
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 7 hours, 8 minutes
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Great spore pics!
You definately want to get "How to Identify Mushrooms to Genus III: Microscopic Features". It goes into great detail on how to make sections, mounting solutions, stains, cystidia types, etc. A must have book for anyone getting into mushroom microscopy and thats most of us, right? 
Paul Stamets' first book "Psilocybe Mushrooms and Their Allies " also has a less detailed but useful microscopy techniques section, which is more specific to Psilocybe examination.
Keep up the good work Allen. I still need to dig out my Psilocybe baeocystis sample. But I did add Psilocybe venenata to the Semilanceatae section gallery.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
Posts: 3,788
Loc: Puget Sound
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Quote:
Alan Rockefeller said: Just based on the spores it looks like a good match for P. subaeruginosa, they are slightly larger and differently shaped than the P. cyanescens spores. It would be interesting to see what the pleurocystidia and cheilocystidia look like.
Care for a gill sample?  I have a few smaller dried samples left. I'd gladly remove a few pieces of gills for you and send them.
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Psilocybe cyanescens, Golden Gate Park

Psilocybe cyanescens, Golden Gate Park

Psilocybe cyanofriscosa, San Francisco

Psilocybe cyanofriscosa, San Francisco
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Psilocybe mexicana Jalisco, Prankster239 collection

Psilocybe mexicana Jalisco, Prankster239 collection

Panaeolus cyanescens, Prankster239 collection

Panaeolus cyanescens, Prankster239 collection
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Psilocybe azurescens, Sporeworks collection

Psilocybe azurescens, Sporeworks collection

Psilocybe villarrealiae, Jalisco, Mexico, Cactu collection

Psilocybe villarrealiae, Jalisco, Mexico, Cactu collection
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CureCat
Strangest


Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
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Re: Spore microscopy photos [Re: Workman]
#7772875 - 12/18/07 05:35 AM (16 years, 1 month ago) |
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Quote:
Workman said: You definately want to get "How to Identify Mushrooms to Genus III: Microscopic Features".
He's got it.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: CureCat]
#7773218 - 12/18/07 09:54 AM (16 years, 1 month ago) |
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Quote:
CureCat said:
Quote:
Workman said: You definately want to get "How to Identify Mushrooms to Genus III: Microscopic Features".
He's got it.
I really wish that was the case. Now everyone knows what to get me for christmas.
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CureCat
Strangest


Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
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Hrrmmm?? Do you have a different edition??
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: CureCat]
#7773582 - 12/18/07 12:12 PM (16 years, 1 month ago) |
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> Hrrmmm?? Do you have a different edition??
No.
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CureCat
Strangest


Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
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Well what book do you have that I am thinking of?! It's got a real plain cover... i thought it was that one.
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canid
irregular meat sprocket




Registered: 02/26/02
Posts: 11,912
Loc: looking for zeebras, n. c...
Last seen: 20 days, 14 hours
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ditto.
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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it. If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: canid]
#7774849 - 12/18/07 06:19 PM (16 years, 1 month ago) |
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Are you thinking of the book with the green cover, The Cytology and Plectology of the Hymenomycetes?
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canid
irregular meat sprocket




Registered: 02/26/02
Posts: 11,912
Loc: looking for zeebras, n. c...
Last seen: 20 days, 14 hours
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haven't seen the book in person, just keep comming across citations of it, exerpts and references in other books and papers.
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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it. If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: canid]
#7775646 - 12/18/07 09:30 PM (16 years, 1 month ago) |
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> haven't seen the book in person, just keep comming across citations of it, exerpts and references in other books and papers.
Its a pretty awesome book, but the price is over $100, I'd recommend you borrow it from someone like I did.
Its a good bet that anyone who buys this book is not reading it, the subject matter is kind of intense.
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canid
irregular meat sprocket




Registered: 02/26/02
Posts: 11,912
Loc: looking for zeebras, n. c...
Last seen: 20 days, 14 hours
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when i have the money i'm not above paying that much for reference material. there are still a couple monograms i want and i've been meaning to pick up a new merck index.
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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it. If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.
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CureCat
Strangest


Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
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Yes, that is the one i was thinking of.
Alan, we should get a dedicated online group of myconauts together to scan as many monographs and other myco references onto one database as possible. How feasible do you think that would be??
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 7 hours, 8 minutes
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Quote:
Alan Rockefeller said:
Its a pretty awesome book, but the price is over $100, I'd recommend you borrow it from someone like I did.
Its a good bet that anyone who buys this book is not reading it, the subject matter is kind of intense.
Mushrooms to Genus III: Microscopic Features is only $24 and it can be found at local libraries.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: Workman]
#7777031 - 12/19/07 10:12 AM (16 years, 1 month ago) |
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> Mushrooms to Genus III: Microscopic Features is only $24 and it can be found at local libraries
I did a search at my local public library for "mushrooms to genus microscopic features" and the first thing that came up was "Wonders of llamas".
So I restrict my search to books by Largent with Mushrooms in the title, and it comes back with "Murder with mushrooms" by John Creasey.
I had no trouble getting it through interlibrary loan.
> Alan, we should get a dedicated online group of myconauts together to scan as many monographs and other myco references onto one database as possible. How feasible do you think that would be??
Sure, I have web server space for it.
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 7 hours, 8 minutes
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To avoid legal hassles, it would be best to keep such a resource from general public access, perhaps by invite only and password protected. And keep the search engines from picking it up.
I have tons of out of print material that I could scan such as Ola'h's 1969 Le Genre Panaeolus which is highly referenced by any papers on Panaeolus as well as Gerhardt's monograph. I also have every reference available on the Panaeolopsis.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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cactu
culture and magic


Registered: 03/06/06
Posts: 3,913
Loc: mexicoelcentrodelconocimi...
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Re: Spore microscopy photos [Re: Workman]
#7777198 - 12/19/07 10:57 AM (16 years, 1 month ago) |
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i´m in ,
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  cuando una rafaga del pensamiento nos pasa al lado se puede sentir que valio la pena haber vivido, y cuando ese pensamiento se convierte en sueño no paramos de soñar hasta realizarlo
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
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Re: Spore microscopy photos [Re: cactu]
#7779634 - 12/19/07 09:30 PM (16 years, 1 month ago) |
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Ok I set up the underground mycology library, pm me for access!
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CureCat
Strangest


Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
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Re: Spore microscopy photos [Re: Workman]
#7780184 - 12/20/07 01:14 AM (16 years, 1 month ago) |
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Quote:
Workman said: I also have every reference available on the Panaeolopsis.
When are you gonna send that to me, hmmm???
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
Last seen: 7 hours, 8 minutes
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Re: Spore microscopy photos [Re: CureCat]
#7781215 - 12/20/07 11:21 AM (16 years, 1 month ago) |
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Hehe, sorry. They are hard copies and still need to be scanned. I'll have them put in the myco library when ready.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: Workman]
#7806177 - 12/28/07 12:00 AM (16 years, 1 month ago) |
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Panaeolus cyanescens, Australia, _OttO_ collection


Psilocybe subaeruginosa, Australia, _OttO_ collection


Psilocybe cubensis albino, Australia, RR rescue, _OttO_ collection


Hydnum repandum with methylene blue stain, Whole Foods collection

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undergrounder
fluffy bunny



Registered: 11/10/06
Posts: 1,394
Loc: Sydney
Last seen: 1 year, 7 months
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Wicked thanks for the aus mushroom pics. I wish i could send you a print from these weird mountain subs

... but i think i got the prints mixed up with some local ones and now i don't know which are which.
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RIP Bigger and bolder and rougher and tougher in other words sucka there is no other...
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Psilocybe semilanceata, 1000x:

Psilocybe semilanceata, 1600x: This is the highest magnification I can do with my current setup, the scope is set to 1600x and the camera is zoomed in to 4x, making the total magnification 6400x.


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CureCat
Strangest


Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
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THEY LOOK LIKE BEANS!!!
j/k j/k. Hehe, so what were the distinguishable micro differences between the Ps. semilanceata and the unknown Panaeolus or Panaeolina?
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casgoodie
weedwright


Registered: 10/31/06
Posts: 770
Loc: terra
Last seen: 10 years, 3 months
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Re: Spore microscopy photos [Re: CureCat]
#7891638 - 01/17/08 02:02 AM (16 years, 16 days ago) |
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i wonder if my local whole foods has hedgies
-------------------- TRAPPED IN LINGUISTIC CONCEPTS
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bmiles
artist



Registered: 12/31/07
Posts: 2,299
Loc: on the left side
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damn, nice post... love the pictures!
-------------------- Never go with a hippy to a second location.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: bmiles]
#7891666 - 01/17/08 02:28 AM (16 years, 16 days ago) |
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Pluteus salicinus spores, Indiana, 1000x, Godflesh collection

> Hehe, so what were the distinguishable micro differences between the Ps. semilanceata and the unknown Panaeolus or Panaeolina?
I don't have the Panaeolus/Psathyrella microscope photos here so I can't directly compare. Psilocybe and Psathyrella spores look very similar, the micro differences will be in the other parts of the mushroom such as the cap cuticle. (pileipellis)
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cactu
culture and magic


Registered: 03/06/06
Posts: 3,913
Loc: mexicoelcentrodelconocimi...
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music to my eyes and candy to my brains thanks i have synesthesia
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  cuando una rafaga del pensamiento nos pasa al lado se puede sentir que valio la pena haber vivido, y cuando ese pensamiento se convierte en sueño no paramos de soñar hasta realizarlo
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Psilocybe subaeruginascens, auweia 01/26/08 collection
Spores, 1000x



Cheilocystidia, 1000x



I noticed something interesting in this specimen - The tips of the cystidia (both cheilo and pleuro types) were often green. At first I thought it was a anomoly, but I saw hundreds of cystidia cells with green tips. When the cystidia is sticking out of a gill edge, it can look like a mushroom with a transparent stem and a green cap. It appears to be staining from psilocin degradation. It doesn't surprise me that random cells contain psilocin that likes to turn blue or green, but it almost looks like psilocin is concentrated in the tips of the cystidia.
Green tipped cheilocystidia can be seen in the next four pics:




Pleurocystidia, 1000x





Edited by Alan Rockefeller (01/28/08 05:26 PM)
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auweia
mountain biking


Registered: 12/03/05
Posts: 2,725
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neato...thanks Alan!
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Subbedhunter420
Solitary Hunter



Registered: 12/30/06
Posts: 1,501
Loc: LA/Ventura County
Last seen: 8 years, 7 months
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Re: Spore microscopy photos [Re: auweia]
#7944887 - 01/28/08 11:31 AM (16 years, 4 days ago) |
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Nice pics alan!
Im getting a microscope when I move out. my family is all excited for me. They want me to take mycology up as a possible career. Dono what the hell im gonna be doing with mushrooms but o well. maybe this.
Inspiration!!!
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: auweia]
#8088578 - 03/01/08 01:29 AM (15 years, 10 months ago) |
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Psilocybe cyanescens, Fakhface collection
Spores, 1000x

Spores, 1000x, three spores in the middle have their germ pores facing up.

Spores, 1000x, end view

Spores, 2800x
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
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Psilocybe villarrealiae spores, 1000x, Prankster239 collection


Psilocybe caerulescens spores, 1000x, Prankster239 collection



The biggest difference I see in the spores between the two species is the color. It is noticeable both looking at the print and through the the microscope. The P. villarrealiae spores are a reddish brown while the P. caerulescens spores are closer to a charcoal gray, just barely if at all brown. After looking at the P. villarrealiae spores in the microscope for awhile, the P. caerulescens looked like chunks of charcoal by comparison.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
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Psilocybe ovoideocystidiata (Belmont) - Shroomydan collection
Scanning electron microscopy by Scout24
1800x:

3000x:

10000x
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CureCat
Strangest


Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
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Whooo, neat-o. Who does Scout know?
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: CureCat]
#9655467 - 01/21/09 10:00 PM (15 years, 10 days ago) |
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Bluing Galerina, Curecat collection, 1000x:


Copelandia cyanescens, mushroomhunter10 collection
100x:

2000x, ten micron divisions:



Psilocybe guilartensis, BlimeyGrimey collection:
100x:

2000x:



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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Gymnopilus luteocarneus, Santa Cruz Fungus Fair 2009
Spores moderately roughened, inequilateral, dextrinoid, no germ pore, 4.5 – 5 × 7 – 8 micrometers.

The spores turned from golden yellow to brick red after being in Melzer's reagent for an hour.

Gymnopilus lutescens
Spores roughened, 7 - 8 x 5 micrometers. These spores are rougher than the spores from G. luteocarneus.


Spores mildly dextrinoid

Gymnopilus flavidellus
Spores 6 – 7 (8) x 4 – 4.5 um, minutely roughened

Spores immediately dextrinoid
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Mr. Mushrooms
Spore Print Collector


Registered: 05/25/08
Posts: 13,018
Loc: Registered: 6/04/02
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I wet myself. Sweet thread, awesome photos. I'm jealous. 
Excellent work. Who identified the Gymnopilus flavidellus?
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Quote:
Excellent work. Who identified the Gymnopilus flavidellus?
I wish I knew. I asked around but no one knew who id'd the gyms.
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Mr. Mushrooms
Spore Print Collector


Registered: 05/25/08
Posts: 13,018
Loc: Registered: 6/04/02
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Hmmm who was that masked man? Well, if you checked the monograph as I did (I'm sure you did) you know the microscopy involved. The species is relatively close to some others.
Very interesting, Holmes. Wouldn't you say?
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Tuber oregonense, Ice House Shaman collection
100x

200x



2000x 10.8 micron divisions

Spores measure 26 - 52 x 24 - 28 micrometers (not counting the ornamentation of course). These are more than twice as big as the largest mushroom spores I have seen!
The spore sacs that have just two spores have much larger spores than the sacs that have 3 or 4 spores. The spores are evenly distributed throughout the inside of the truffle.
Here are some pics of the black truffle spores




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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Curecat collection, tiny Psilocybe species 12/6/08
Three normal sized spores

The spores are inequilateral, almost diamond shaped in this view

Three more average sized spores

A really big spore! This one measures 16.21 x 7.28 um

Two larger than average spores

A really big spore next to a normal size spore. 90% of the spores were the smaller size and a few are much larger (maybe from 2 spored basidia?)


Spore measurements: 10.92 x 6.19 11.19 x 6.12 11.81 x 5.89 12.02 x 6.58 12.37 x 6.77 12.51 x 6.03 12.63 x 6.87 12.65 x 5.94 12.66 x 7.17 12.72 x 6.59 13.65 x 6.82 13.74 x 6.58 16.09 x 7.52 16.21 x 7.28
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Stropharia riparia spores, 100x
2/12/09
Mountain View, CA
Darkfield illumination
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Mr. Mushrooms
Spore Print Collector


Registered: 05/25/08
Posts: 13,018
Loc: Registered: 6/04/02
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Beautiful stuff. Thanks for sharing.
Quote:
Spore measurements: 10.92 x 6.19 11.19 x 6.12 11.81 x 5.89 12.02 x 6.58 12.37 x 6.77 12.51 x 6.03 12.63 x 6.87 12.65 x 5.94 12.66 x 7.17 12.72 x 6.59 13.65 x 6.82 13.74 x 6.58 16.09 x 7.52 16.21 x 7.28
Incredibly accurate measurements too. Nice.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Gymnopilus spores, shroomgatherer collection 2/21/09

Psilocybe subcubensis, EQ print

Psilocybe subaeruginascens, coolboarderguy collection

Leptonia incana, rickstar collection
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Alan Rockefeller
Mycologist

Registered: 03/10/07
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Last seen: 14 minutes, 11 seconds
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 5 months
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nice work man. did you ever get a chance to look at those 3 Panaeolous and that Stropharia for me from 2011?
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Quote:
psylosymonreturns said: did you ever get a chance to look at those 3 Panaeolous and that Stropharia for me from 2011?
Yes, they were kind of boring. I think all your Panaeolus samples are the same. I took a bunch of pictures but have not uploaded them yet. I couldn't tell if the spores were smooth or very minutely ornamented, so I guess I should call them smooth. If that is the case, they would fall into the Panaeolus cinctulus bucket. I have your samples here and I have much better microscopes now so I might take another look at them soon.
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 5 months
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wow cinctulus eh! i was thinking one was for sure P olivaceus , another one was a real cool one macroscopicly resembling P fimicola and the last one i now am pretty sure is just P acuminatus. But the spores dont lie i guess ! maybe your new scope will see something different though.
thanks so much for looking at them for me .
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Alan Rockefeller
Mycologist

Registered: 03/10/07
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Last seen: 14 minutes, 11 seconds
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Psilocybe allenii 1000x



ITS Sequence: http://www.ncbi.nlm.nih.gov/nuccore/HE994450.1
Spore measurements:
10.9 [11.9 ; 12.3] 13.2 × 6.1 [6.7 ; 6.9] 7.5 µm Q = 1.6 [1.7 ; 1.8] 2 ; N = 35 ; C = 95% Me = 12.1 × 6.8 µm ; Qe = 1.8
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BjJiggles
Gym seeker



Registered: 03/13/12
Posts: 2,736
Loc:
Last seen: 8 years, 25 days
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How are you getting these microscope images? Are you using an electron microscope? Maybe the answer is obvious, but unfortunately I don't have the resources to be very familiar with Electron microscopes...
The images are beautiful btw, can we expect much more microscopy, of this quality, to come?
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 Alan Rockefeller said:No! Do not feed the type collection of a new species to animals!
Edited by BjJiggles (02/26/13 10:21 AM)
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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Re: Spore microscopy photos [Re: BjJiggles]
#17868277 - 02/26/13 10:21 AM (10 years, 10 months ago) |
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BjJiggles
Gym seeker



Registered: 03/13/12
Posts: 2,736
Loc:
Last seen: 8 years, 25 days
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That camera looks awesome, I've been wanting one like that, that will mount in the eyepiece
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 Alan Rockefeller said:No! Do not feed the type collection of a new species to animals!
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: BjJiggles]
#17869336 - 02/26/13 01:57 PM (10 years, 10 months ago) |
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Quote:
BjJiggles said: How are you getting these microscope images? Are you using an electron microscope? Maybe the answer is obvious, but unfortunately I don't have the resources to be very familiar with Electron microscopes...
I used a Nikon Eclipse 80i with DIC prisms and objectives.
Quote:
The images are beautiful btw, can we expect much more microscopy, of this quality, to come?
Occasionally, when I take my samples to a college with a $50,000 scope.
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BjJiggles
Gym seeker



Registered: 03/13/12
Posts: 2,736
Loc:
Last seen: 8 years, 25 days
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That Nikon is a badass scope(read about it). Is that your personal microscope?
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 Alan Rockefeller said:No! Do not feed the type collection of a new species to animals!
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Re: Spore microscopy photos [Re: BjJiggles]
#17869536 - 02/26/13 02:30 PM (10 years, 10 months ago) |
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Quote:
BjJiggles said: Is that your personal microscope?
No.
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,276
Last seen: 14 minutes, 11 seconds
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Lepiota spheniscispora in Melzers
Pseudobaeospora
Lactarius fragilis var. rubidus
Lepiota castanedisca
Psilocybe cyanescens
Lactarius pallescens
Ramaria formosa
Inocybe kaufmanii http://mushroomobserver.org/122809
Inocybe grammata http://mushroomobserver.org/122814
Psathyrella ulignicicola sensu PNW http://mushroomobserver.org/123283
Tomentella sp.
http://mushroomobserver.org/123177
Cortinarius subgenus Leprocybe
Pholiotina teneroides
http://mushroomobserver.org/127696
Psilocybe muliercula
The first record of this species from Oaxaca
http://mushroomobserver.org/114470
Pholiota sp. from a landslide — in San José Del Pacífico, Oaxaca, Mexico.
Psilocybe subtropicalis
http://mushroomobserver.org/127923 — in Coatepec, Veracruz-Llave, Mexico.
Trichoglossum hirsutum
http://mushroomobserver.org/129406 — at Muir Woods National Monument.
Psilocybe subaeruginascens
http://mushroomobserver.org/128946
An undescribed Otidea species
http://mushroomobserver.org/115972

Pleurotus ostreatus 1000x — at Muir Woods National Monument.

Psilocybe allenii
http://mushroomobserver.org/26833

Psilocybe allenii
http://mushroomobserver.org/58787
Craterellus cornucopioides 1000x — at Muir Woods National Monument.
Craterellus cornucopioides 1000x — at Muir Woods National Monument.
Psilocybe yungensis spores 1000x

Pleurotus ostreatus 400x phase contrast

Psilocybe mexicana spores and cheilocystidia 400x phase contrast — with Pedro Paramo at San Agustin Loxicha, Oaxaca, Mexico.

Psilocybe mexicana spores and cheilocystidia 400x phase contrast — at San Agustin Loxicha, Oaxaca, Mexico.
Psilocybe pelliculosa
Trichoglossum hirsutum
http://mushroomobserver.org/129406 — at Muir Woods National Monument.

Trichoglossum hirsutum
http://mushroomobserver.org/129406 — at Muir Woods National Monument.
Trichoglossum hirsutum
http://mushroomobserver.org/129406 — at Muir Woods National Monument.
Psilocybe cubensis, from a print labeled "Panama"

Gymnopilus, from a print labeled "Gymnopilus aeruginosus"
Psilocybe cyanescens darkfield 200x

Battarrea phalloides?
http://mushroomobserver.org/129748
Trichoglossum hirsutum
http://en.wikipedia.org/wiki/Trichoglossum_hirsutum
Psilocybe allenii cheilocystidia 600x
http://mushroomobserver.org/58787
Psilocybe allenii cheilocystidia 600x
http://mushroomobserver.org/58787
Psilocybe allenii cheilocystidia 600x
http://mushroomobserver.org/58787
Psilocybe allenii cheilocystidia 600x
http://mushroomobserver.org/58787
Psilocybe allenii cheilocystidia darkfield 600x
http://mushroomobserver.org/58787

Psilocybe subaeruginosa cheilocystidia 600x
http://mushroomobserver.org/88182
Psilocybe cyanescens spores 1000x
http://mushroomobserver.org/119071
Psilocybe subaeruginosa spores 1000x
http://mushroomobserver.org/88182
Psilocybe baeocystis spores 1000x
http://mushroomobserver.org/130071
Gymnopilus luteofolius gill crush mount
Psilocybe quebecensis spores and cheilocystidia 1000x
Psilocybe quebecensis pleurocystidia 600x

Psilocybe quebecensis cheilocystidia 600x
Psilocybe baeocystis spores 1000x
http://mushroomobserver.org/130071

Tulostoma clathrosporum
http://mushroomobserver.org/129972
Panaeolus olivaceus 1000x
http://mushroomobserver.org/129971
Panaeolus olivaceus 1000x
http://mushroomobserver.org/129971
Asterophora lycoperdoides 1000x
http://mushroomobserver.org/127934
Asterophora lycoperdoides 1000x
http://mushroomobserver.org/127934

Tuber gibbosum
http://mushroomobserver.org/130478 — in Portland, Oregon.
Amanita section Vaginatae http://mushroomobserver.org/143883

Crepidotus
http://mushroomobserver.org/143631
A new species of Amanita from section Lepidella http://mushroomobserver.org/143625

Entoloma

Cronartium quercuum
http://mushroomobserver.org/143627
Psilocybe subtropicalis 1000x
http://mushroomobserver.org/210888
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Lhun
Fungal Fixation



Registered: 01/07/10
Posts: 2,106
Loc: Other side of your screen...
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Wow, epic update to an old classic thread. Good stuff Alan!
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