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OfflineAlan RockefellerM
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Spore microscopy photos
    #7760612 - 12/14/07 11:31 PM (16 years, 1 month ago)

I got some spores in the mail from BlimeyGrimey and took some pictures of them.

Condensation on a petri dish cover:



The rest of these pics are at 1000x. No zooming or photo editing was done so the spore sizes can be compared.

Psilocybe stuntzii. These have small subrhomboid spores which look very similar to P. ovoideocystidiata or P. subaeruginascens spores:



Another shot of P. stuntzii spores:



This print was labeled Psilocybe cyanofibrillosa specimen C - Seattle, WA. The spores are much larger than the spores of P. cyanofibrillosa, they look about the same size as P. cyanescens. The mushroom that made these spores is probably in the P. cyanofriscosa complex.



P. cyanescens spores, Seattle, WA:



G. luteofolius spores. I compared them with G. luteofolius spores from my collection and they matched. These spores are finely warted, as you can see in a few of the spores which are at the right height to be focused on the outer layer.



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OfflineILLegal
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7760617 - 12/14/07 11:34 PM (16 years, 1 month ago)

Quote:

Alan Rockefeller said:









Is it just me or does the picture seem to move?

Oh and nice by the way.


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: ILLegal]
    #7760883 - 12/15/07 03:00 AM (16 years, 1 month ago)

Here is a gill edge from a Gymnopilus I found in a log in GGP:



Psilocybe atlantis spores:



More Psilocybe atlantis spores:



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InvisibleBlimeyGrimey
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7760948 - 12/15/07 04:44 AM (16 years, 1 month ago)

Quote:

Alan Rockefeller said:
This print was labeled Psilocybe cyanofibrillosa specimen C - Seattle, WA. The spores are much larger than the spores of P. cyanofibrillosa, they look about the same size as P. cyanescens. The mushroom that made these spores is probably in the P. cyanofriscosa complex.







Here's a pic of the cyano's.



You can see specimen "C" to the right. It was hidden under weeds next to specimen "B".

They do not resemble cyanescens while in their immature or mature phases. Here's a pic of the smaller ones I found.




I believe I have found the same species that SporeWorks has listed as cyanofibrillosa, as Workman has said that their collection is infact NOT cyanofibrillosa. He has been sent dried gill samples to further identify this species.

Thanks Rockefeller! I knew these cyanofubs seemed a bit different than the macroscopic textbook description of fibs. Now we have microscopic proof also.

Another thanks for verifying my luetofolius find. I was unsure as to whether they were luetofolius or aeruginosus.

Sorry for the VERY VERY small stunztii print but it was all I had left other than my last decent print. Glad there was enough spores for you to work with.

Did the stuntzii spores match up to the textbook description of the spores?

Pics of the stuntzii I found last year and this year.

This year's pic:


Last year's pic (from the same patch):



Thanks again Rockefeller! We need more people like you with camera's for their scopes. I hope to get one soon!


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Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.


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Offlineundergrounder
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Re: Spore microscopy photos [Re: BlimeyGrimey]
    #7760979 - 12/15/07 05:17 AM (16 years, 1 month ago)

Quote:

BlimeyGrimey said:
Here's a pic of the cyano's.



You can see specimen "C" to the right. It was hidden under weeds next to specimen "B".

They do not resemble cyanescens while in their immature or mature phases. Here's a pic of the smaller ones I found.






Those look a lot like the colder growing Ps. subaeruginosa we get here. They don't seem to open up fully, and they seem more chunky than their UFO or wavy-shaped cousins.


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:igor: RIP :igor:

Bigger and bolder and rougher and tougher in other words sucka there is no other...
:pinkshroom: :supershroom: :mushroom2: :shroomer: :mushroom2: :supershroom: :pinkshroom:


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InvisibleBlimeyGrimey
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Re: Spore microscopy photos [Re: undergrounder]
    #7760994 - 12/15/07 05:43 AM (16 years, 1 month ago)

I showed those mushrooms to a friend and they said they looked like Ps. subaeruginosa. When I told them where they had been picked he then said they were probably cyanofibrillosa.

Alan, do you have a description of Ps. subaeruginosa to compare those cyanofib spores to?


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Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.


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Invisiblesui
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Re: Spore microscopy photos [Re: BlimeyGrimey]
    #7761203 - 12/15/07 09:02 AM (16 years, 1 month ago)

fucking NICE Alan!


--------------------

"There is never a wrong note, bend it."
Jimi Hendrix



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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: BlimeyGrimey]
    #7761617 - 12/15/07 12:20 PM (16 years, 1 month ago)

> I believe I have found the same species that SporeWorks has listed as cyanofibrillosa, as Workman has said that their collection is infact NOT cyanofibrillosa. He has been sent dried gill samples to further identify this species.

Yes, good chance it is that one.

> Did the stuntzii spores match up to the textbook description of the spores?

Yes, they do.

> Another thanks for verifying my luetofolius find. I was unsure as to whether they were luetofolius or aeruginosus.

The spores for those two species are identical.

> Those look a lot like the colder growing Ps. subaeruginosa we get here. They don't seem to open up fully, and they seem more chunky than their UFO or wavy-shaped cousins.

> I showed those mushrooms to a friend and they said they looked like Ps. subaeruginosa. When I told them where they had been picked he then said they were probably cyanofibrillosa.

> Alan, do you have a description of Ps. subaeruginosa to compare those cyanofib spores to?

P. cyanescens spores are 9-12 x 5 - 8, elongate-ellipsoid while P. subaeruginosa has spores that are 13-15 x 6.6 - 7.6 microns and have a subellipsoid shape.

Just based on the spores it looks like a good match for P. subaeruginosa, they are slightly larger and differently shaped than the P. cyanescens spores. It would be interesting to see what the pleurocystidia and cheilocystidia look like.


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7761621 - 12/15/07 12:22 PM (16 years, 1 month ago)

Here are two pictures of P. subaeruginascens spores, not to be confused with P. subaeruginosa.

These are from a GGP collection. The spores a small and subrhomboid, very similar to P. stuntzii spores.





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Offline2859558484
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7761692 - 12/15/07 12:42 PM (16 years, 1 month ago)

wow,alan you are fucking cool. keep it up.



this is like a free mycology class with a badass professor that smokes blueberry chron with you.


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Offlinenever_2_high
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7761694 - 12/15/07 12:42 PM (16 years, 1 month ago)

Que lindas fotos Alan! Me encantan.:thumbup:


Edited by never_2_high (12/15/07 03:05 PM)


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Offlinecasgoodie
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Re: Spore microscopy photos [Re: never_2_high]
    #7761981 - 12/15/07 02:23 PM (16 years, 1 month ago)

thanks for sharing, much appreciated.


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TRAPPED IN LINGUISTIC CONCEPTS


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InvisibleGGreatOne234
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Re: Spore microscopy photos [Re: casgoodie]
    #7762443 - 12/15/07 04:39 PM (16 years, 1 month ago)

Beautiful pictures Alan.

You're a mad-scientist hehehe:)

I honestly do not know too much about microscopic features of mushrooms yet. I do not have a microscope (yet), but that would be the end of the line officially making me a mycological wack-job. It'd be too intense for me lol. After seeing your cool images and reading your microscopy-related mushroom-definitions (of which I am not very familiar with), it makes me want to get the "How to Identify Mushrooms to Genus" book involving microscopy. Is that the book from where you started to learn all this? It seems like the only book I know of that deals with the topic in depth.

I'm still trying to digest "How to Identfy Mushrooms to Genus" parts one and two..

Hey great stuff man, good work, thanks for sharing.


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OfflineSubbedhunter420
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Re: Spore microscopy photos [Re: GGreatOne234]
    #7762527 - 12/15/07 05:05 PM (16 years, 1 month ago)

Those are some amazing pics alan! youve inspired me to buy my own microscope as soon as i can to start working on the panaeolus down here.


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Invisiblecactu
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Re: Spore microscopy photos [Re: Subbedhunter420]
    #7762726 - 12/15/07 06:01 PM (16 years, 1 month ago)

HOLA SEÑOR ALANROCKEFELLER me dirigo ante usted de la manera mas cordial , para ver la posibilidad de enviarle un gran saludo , de agradecerle el trabajo tan duro y constante que realiza para todos nosotros, me da mucho gusto observas sus fotos, me llevan a lugares desconocidos de mi mente y me conectan directamente con el destino, yo soy un poeta señor , y sus acciones me inspiran a crear las lineas mas intricadas en los misterios del bosque , pero en esta ocasion quiero preguntarle si seria oposible mandarle cierto material para mantener la esperanza y la concentracion fijada en un solo punto , me gustaria saber por que no hay basidios en estas fotos , pero a lo mejor no conto con dicho material , por lo que prontamente me agradaria mandarle un material de trabajo nuevo ,
me despido de usted deseandole que sus sueños se hagan realidad.
para usted mis mejores vibraciones .......


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cuando una rafaga del pensamiento nos pasa  al lado se puede sentir  que valio  la pena  haber vivido, y cuando ese pensamiento se  convierte en sueño no paramos de soñar hasta realizarlo


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InvisibleGGreatOne234
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Re: Spore microscopy photos [Re: cactu]
    #7762751 - 12/15/07 06:07 PM (16 years, 1 month ago)

ehhhh-cactu,, I was too stoned every morning during first-period Spanish class in high school to make heads or tails of all-that.. took two years of Spanish, always first period.. and believe me when I say that constantly smoking bongs at home while trying to memorize words in a different language doesn't work out so well for me.


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OfflineRekkuzo
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Re: Spore microscopy photos [Re: cactu]
    #7763001 - 12/15/07 07:36 PM (16 years, 1 month ago)

Quote:

cactu said:
HOLA SEÑOR ALANROCKEFELLER me dirigo ante usted de la manera mas cordial , para ver la posibilidad de enviarle un gran saludo , de agradecerle el trabajo tan duro y constante que realiza para todos nosotros, me da mucho gusto observas sus fotos, me llevan a lugares desconocidos de mi mente y me conectan directamente con el destino, yo soy un poeta señor , y sus acciones me inspiran a crear las lineas mas intricadas en los misterios del bosque , pero en esta ocasion quiero preguntarle si seria oposible mandarle cierto material para mantener la esperanza y la concentracion fijada en un solo punto , me gustaria saber por que no hay basidios en estas fotos , pero a lo mejor no conto con dicho material , por lo que prontamente me agradaria mandarle un material de trabajo nuevo ,
me despido de usted deseandole que sus sueños se hagan realidad.
para usted mis mejores vibraciones .......




Translation:
Hello, Mr. Alan Rockefeller. I bring myself before you in the most cordial manner, to see the possibility of sending you a grand greeting, to thank you for the constant and hard work that you do for us, I very much enjoy looking at your pictures, they take me to unknown places within my mind and connect me directly to destiny, I am a poet sir, and your actions inspire me to create more intricate lines on the mysteries of the forest, but in this occasion I want to ask if it would be possible(typo?) to send you certain material to keep your hope and and concentration fixated on one single point, I would like to know why there aren't basidia in those photos, but on the other hand maybe I shouldn't count on said material, for I would promptly like to send you another subject to study, I bid you farewell now, wishing that your dreams become reality. All my best vibrations.


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InvisibleGGreatOne234
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Re: Spore microscopy photos [Re: Rekkuzo]
    #7763058 - 12/15/07 07:57 PM (16 years, 1 month ago)

OOooo, la-dee-freakin-doo-da!

How many years did you study spanish to translate like that?

Nicely done i must say


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Invisiblecactu
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Re: Spore microscopy photos [Re: GGreatOne234]
    #7763066 - 12/15/07 08:01 PM (16 years, 1 month ago)

hola GG234 vas a tener que repasar tus lecciones de español una ves mas , he comprobado en mis muchas formas de aprender ingles que fumar incrementa la atencion subjetiva y me ayuda a expresarme , ademas de ser una herramienta basica para conectar con los espiritus de los hongos cuando estoy en la busqueda de ellos , sabes que estoy haciendo esto para que vallas practicando y no sea dificil decir
ME ENCANTA MEXICO , OTRO MAS POR FAVOR, LA VIDA ES VIDA Y CON HONGOS SUPER VIDA ......COSAS POR EL ESTILO .
WELL ALL MY BEST VIBRATIONS FROM THE OTHER SIDE ..........


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cuando una rafaga del pensamiento nos pasa  al lado se puede sentir  que valio  la pena  haber vivido, y cuando ese pensamiento se  convierte en sueño no paramos de soñar hasta realizarlo


Edited by cactu (12/15/07 08:12 PM)


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: GGreatOne234]
    #7763079 - 12/15/07 08:08 PM (16 years, 1 month ago)

Hypholoma sublateritium, 1000x, zoomed out:



Hypholoma sublateritium, 1000x, zoomed in:



Gymnopilus luteofolius, 1000x, zoomed in:



G. luteofolius, 1000s, zoomed in:



Psilocybe zapotecorum from Colima, Mexico:



P. zapotecorum Colima:



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OfflineRekkuzo
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Re: Spore microscopy photos [Re: GGreatOne234]
    #7763080 - 12/15/07 08:08 PM (16 years, 1 month ago)

Lol, born in Puerto Rico, no study required.

Hola cactu, me encantan tus fotos.


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InvisibleBlimeyGrimey
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Re: Spore microscopy photos [Re: GGreatOne234]
    #7763107 - 12/15/07 08:15 PM (16 years, 1 month ago)

Babelfish translates it in less then 2 seconds .....


Rekkuzo:
Nice to see another puerto rican on the forums! Unfortunately I only lived there for 6 months as a baby then moved to the mainland.


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Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.


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OfflineRekkuzo
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Re: Spore microscopy photos [Re: BlimeyGrimey]
    #7763136 - 12/15/07 08:21 PM (16 years, 1 month ago)

I moved shortly after my 7th birthday. I kept all my Spanish skillz, but my vocabulary is pretty limited, though speaking with my parents constantly helps.

Lol, I just checked what babelfish would come up with, and it's completely bastardized.

Also, why do the zapotecorum spores look all "dirty"?


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Invisiblecactu
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Re: Spore microscopy photos [Re: BlimeyGrimey]
    #7763154 - 12/15/07 08:23 PM (16 years, 1 month ago)

hola rekkuzo , gracias  por lo de las fotos y la traduccion para el señor alan rocketfeller, ahh, el español es tan bonito y poetico mucho mas que el ingles , pero bueno hay  que ponerle salsa al foro deves encuando no .,:grin:
saludos  my best vibration for you i bet spores look dirty  due to alan travel method


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cuando una rafaga del pensamiento nos pasa  al lado se puede sentir  que valio  la pena  haber vivido, y cuando ese pensamiento se  convierte en sueño no paramos de soñar hasta realizarlo


Edited by cactu (12/15/07 08:25 PM)


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: cactu]
    #7764852 - 12/16/07 10:24 AM (16 years, 1 month ago)

> wow,alan you are fucking cool. keep it up.
> this is like a free mycology class with a badass professor that smokes blueberry chron with you.

Thanks, that is exactly what I was going for.

> You're a mad-scientist hehehe:)

Haha you should see my garage.

> I do not have a microscope (yet), but that would be the end of the line officially making me a mycological wack-job.

Sounds like a good reason to get one.

Plus, the chicks really dig them.

> it makes me want to get the "How to Identify Mushrooms to Genus" book involving microscopy. Is that the book from where you started to learn all this?

No, but I plan to get those next.

> It seems like the only book I know of that deals with the topic in depth.

I am reading The Cytology and Plectology of the Hymenomycetes.

> Those are some amazing pics alan! youve inspired me to buy my own microscope as soon as i can to start working on the panaeolus down here.

I have this one, its great. I have a camera mounted to its third eye now, its fun to sit back and pan around the slide, watching the spores fly across the LCD screen in the camera.

> i bet spores look dirty due to alan travel method

The print is really light, Its hard to get a good sample on to the slide without scraping up some paper too.


Bluing Pluteus species, Curecat collection, CA


Bluing Pluteus species, Curecat collection, CA


Psilocybe cubensis, Waxingren lawn clippings collection


Psilocybe cubensis, Waxingren lawn clippings collection


Panaeolus subbalteatus, subbedhunter420 collection


Panaeolus subbalteatus, subbedhunter420 collection


Panaeolus subbalteatus, subbedhunter420 collection


Psilocybe ovoideocystidiata - Belmont - Shroomydan collection


Psilocybe ovoideocystidiata - Belmont - Shroomydan collection


Psilocybe ovoideocystidiata - Bethany - Shroomydan collection


Psilocybe ovoideocystidiata - Bethany - Shroomydan collection


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Invisiblecactu
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7765070 - 12/16/07 11:27 AM (16 years, 1 month ago)

:sun:you are omnipresent alan , :psychsplit:
shroomery have the best animations  :patriot:  :yourock:
:froman:
are you gonna past the  line with  such a beautiful introduction to  another world ,..:blush:
all  my best vibrations, do you have a pcr stickiest devise, to perform genome exchange in interest into mutated to  the next genus. or at least  initiate a symbiotic relationship  even in intracellular linking , so far i have only try the old methods . but  will interest to see if there is Good machine to id  all mushrooms with a projection to the future and back and give you all details in present time..  you see i´m getting  crazy , yes is the dry season  symptoms affect all my brains i have 2 , i guess is the lack of moisture, or to little  excitement, but the cyber  world feed me........


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cuando una rafaga del pensamiento nos pasa  al lado se puede sentir  que valio  la pena  haber vivido, y cuando ese pensamiento se  convierte en sueño no paramos de soñar hasta realizarlo


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OfflineWorkmanV
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7765583 - 12/16/07 01:27 PM (16 years, 1 month ago)

Great spore pics!

You definately want to get "How to Identify Mushrooms to Genus III: Microscopic Features".  It goes into great detail on how to make sections, mounting solutions, stains, cystidia types, etc.  A must have book for anyone getting into mushroom microscopy and thats most of us, right? :wink:


Paul Stamets' first book "Psilocybe Mushrooms and Their Allies " also has a less detailed but useful microscopy techniques section, which is more specific to Psilocybe examination.

Keep up the good work Allen. I still need to dig out my Psilocybe baeocystis sample.  But I did add Psilocybe venenata to the Semilanceatae section gallery.


--------------------
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InvisibleBlimeyGrimey
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7767520 - 12/16/07 09:38 PM (16 years, 1 month ago)

Quote:

Alan Rockefeller said:
Just based on the spores it looks like a good match for P. subaeruginosa, they are slightly larger and differently shaped than the P. cyanescens spores.  It would be interesting to see what the pleurocystidia and cheilocystidia look like.




Care for a gill sample? :stoned:
I have a few smaller dried samples left. I'd gladly remove a few pieces of gills for you and send them.


--------------------
Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: BlimeyGrimey]
    #7771922 - 12/17/07 10:05 PM (16 years, 1 month ago)

Psilocybe cyanescens, Golden Gate Park



Psilocybe cyanescens, Golden Gate Park



Psilocybe cyanofriscosa, San Francisco



Psilocybe cyanofriscosa, San Francisco



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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7772024 - 12/17/07 10:28 PM (16 years, 1 month ago)

Psilocybe mexicana Jalisco, Prankster239 collection


Psilocybe mexicana Jalisco, Prankster239 collection


Panaeolus cyanescens, Prankster239 collection


Panaeolus cyanescens, Prankster239 collection


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7772528 - 12/18/07 01:09 AM (16 years, 1 month ago)

Psilocybe azurescens, Sporeworks collection



Psilocybe azurescens, Sporeworks collection



Psilocybe villarrealiae, Jalisco, Mexico, Cactu collection



Psilocybe villarrealiae, Jalisco, Mexico, Cactu collection



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Re: Spore microscopy photos [Re: Workman]
    #7772875 - 12/18/07 05:35 AM (16 years, 1 month ago)

Quote:

Workman said:
You definately want to get "How to Identify Mushrooms to Genus III: Microscopic Features".



He's got it.


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: CureCat]
    #7773218 - 12/18/07 09:54 AM (16 years, 1 month ago)

Quote:

CureCat said:
Quote:

Workman said:
You definately want to get "How to Identify Mushrooms to Genus III: Microscopic Features".



He's got it.




I really wish that was the case. Now everyone knows what to get me for christmas.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7773249 - 12/18/07 10:02 AM (16 years, 1 month ago)

Hrrmmm?? Do you have a different edition??


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: CureCat]
    #7773582 - 12/18/07 12:12 PM (16 years, 1 month ago)

> Hrrmmm?? Do you have a different edition??

No.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7774739 - 12/18/07 05:50 PM (16 years, 1 month ago)

Well what book do you have that I am thinking of?! It's got a real plain cover... i thought it was that one.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7774772 - 12/18/07 05:59 PM (16 years, 1 month ago)

ditto.


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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


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Re: Spore microscopy photos [Re: canid]
    #7774849 - 12/18/07 06:19 PM (16 years, 1 month ago)

Are you thinking of the book with the green cover, The Cytology and Plectology of the Hymenomycetes?


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7774937 - 12/18/07 06:39 PM (16 years, 1 month ago)

haven't seen the book in person, just keep comming across citations of it, exerpts and references in other books and papers.


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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


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Re: Spore microscopy photos [Re: canid]
    #7775646 - 12/18/07 09:30 PM (16 years, 1 month ago)

> haven't seen the book in person, just keep comming across citations of it, exerpts and references in other books and papers.

Its a pretty awesome book, but the price is over $100, I'd recommend you borrow it from someone like I did.

Its a good bet that anyone who buys this book is not reading it, the subject matter is kind of intense.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7775880 - 12/18/07 10:43 PM (16 years, 1 month ago)

when i have the money i'm not above paying that much for reference material. there are still a couple monograms i want and i've been meaning to pick up a new merck index.


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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7776205 - 12/19/07 12:54 AM (16 years, 1 month ago)

Yes, that is the one i was thinking of.

Alan, we should get a dedicated online group of myconauts together to scan as many monographs and other myco references onto one database as possible. How feasible do you think that would be??


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7776932 - 12/19/07 09:42 AM (16 years, 1 month ago)

Quote:

Alan Rockefeller said:

Its a pretty awesome book, but the price is over $100, I'd recommend you borrow it from someone like I did.

Its a good bet that anyone who buys this book is not reading it, the subject matter is kind of intense.




Mushrooms to Genus III: Microscopic Features
is only $24 and it can be found at local libraries.


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Workman]
    #7777031 - 12/19/07 10:12 AM (16 years, 1 month ago)

> Mushrooms to Genus III: Microscopic Features
is only $24 and it can be found at local libraries

I did a search at my local public library for "mushrooms to genus microscopic features" and the first thing that came up was "Wonders of llamas".

So I restrict my search to books by Largent with Mushrooms in the title, and it comes back with "Murder with mushrooms" by John Creasey.

I had no trouble getting it through interlibrary loan.

> Alan, we should get a dedicated online group of myconauts together to scan as many monographs and other myco references onto one database as possible. How feasible do you think that would be??

Sure, I have web server space for it.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7777082 - 12/19/07 10:27 AM (16 years, 1 month ago)

To avoid legal hassles, it would be best to keep such a resource from general public access, perhaps by invite only and password protected. And keep the search engines from picking it up.

I have tons of out of print material that I could scan such as Ola'h's 1969 Le Genre Panaeolus which is highly referenced by any papers on Panaeolus as well as Gerhardt's monograph. I also have every reference available on the Panaeolopsis.


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Re: Spore microscopy photos [Re: Workman]
    #7777198 - 12/19/07 10:57 AM (16 years, 1 month ago)

i´m  in , :pandorasbox:


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cuando una rafaga del pensamiento nos pasa  al lado se puede sentir  que valio  la pena  haber vivido, y cuando ese pensamiento se  convierte en sueño no paramos de soñar hasta realizarlo


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Re: Spore microscopy photos [Re: cactu]
    #7779634 - 12/19/07 09:30 PM (16 years, 1 month ago)

Ok I set up the underground mycology library, pm me for access!


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Re: Spore microscopy photos [Re: Workman]
    #7780184 - 12/20/07 01:14 AM (16 years, 1 month ago)

Quote:

Workman said:
I also have every reference available on the Panaeolopsis.



When are you gonna send that to me, hmmm???


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Re: Spore microscopy photos [Re: CureCat]
    #7781215 - 12/20/07 11:21 AM (16 years, 1 month ago)

Hehe, sorry. They are hard copies and still need to be scanned. I'll have them put in the myco library when ready.


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Re: Spore microscopy photos [Re: Workman]
    #7806177 - 12/28/07 12:00 AM (16 years, 1 month ago)

Panaeolus cyanescens, Australia, _OttO_ collection





Psilocybe subaeruginosa, Australia, _OttO_ collection





Psilocybe cubensis albino, Australia, RR rescue, _OttO_ collection





Hydnum repandum with methylene blue stain, Whole Foods collection





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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7806644 - 12/28/07 06:01 AM (16 years, 1 month ago)

Wicked thanks for the aus mushroom pics. I wish i could send you a print from these weird mountain subs



... but i think i got the prints mixed up with some local ones and now i don't know which are which.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7891563 - 01/17/08 01:25 AM (16 years, 16 days ago)

Psilocybe semilanceata, 1000x:



Psilocybe semilanceata, 1600x: This is the highest magnification I can do with my current setup, the scope is set to 1600x and the camera is zoomed in to 4x, making the total magnification 6400x.







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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7891614 - 01/17/08 01:42 AM (16 years, 16 days ago)

THEY LOOK LIKE BEANS!!!

j/k j/k. Hehe, so what were the distinguishable micro differences between the Ps. semilanceata and the unknown Panaeolus or Panaeolina?


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Re: Spore microscopy photos [Re: CureCat]
    #7891638 - 01/17/08 02:02 AM (16 years, 16 days ago)

i wonder if my local whole foods has hedgies


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7891657 - 01/17/08 02:14 AM (16 years, 16 days ago)

damn, nice post... love the pictures!


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: bmiles]
    #7891666 - 01/17/08 02:28 AM (16 years, 16 days ago)

Pluteus salicinus spores, Indiana, 1000x, Godflesh collection



> Hehe, so what were the distinguishable micro differences between the Ps. semilanceata and the unknown Panaeolus or Panaeolina?

I don't have the Panaeolus/Psathyrella microscope photos here so I can't directly compare. Psilocybe and Psathyrella spores look very similar, the micro differences will be in the other parts of the mushroom such as the cap cuticle. (pileipellis)


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7899396 - 01/18/08 09:44 PM (16 years, 14 days ago)

music to my eyes and candy to my brains thanks i have synesthesia


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cuando una rafaga del pensamiento nos pasa  al lado se puede sentir  que valio  la pena  haber vivido, y cuando ese pensamiento se  convierte en sueño no paramos de soñar hasta realizarlo


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7943826 - 01/28/08 02:18 AM (16 years, 5 days ago)

Psilocybe subaeruginascens, auweia 01/26/08 collection

Spores, 1000x







Cheilocystidia, 1000x







I noticed something interesting in this specimen - The tips of the cystidia (both cheilo and pleuro types) were often green. At first I thought it was a anomoly, but I saw hundreds of cystidia cells with green tips. When the cystidia is sticking out of a gill edge, it can look like a mushroom with a transparent stem and a green cap. It appears to be staining from psilocin degradation. It doesn't surprise me that random cells contain psilocin that likes to turn blue or green, but it almost looks like psilocin is concentrated in the tips of the cystidia.

Green tipped cheilocystidia can be seen in the next four pics:









Pleurocystidia, 1000x













Edited by Alan Rockefeller (01/28/08 05:26 PM)


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #7944230 - 01/28/08 08:13 AM (16 years, 4 days ago)

neato...thanks Alan!


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Re: Spore microscopy photos [Re: auweia]
    #7944887 - 01/28/08 11:31 AM (16 years, 4 days ago)

Nice pics alan!

Im getting a microscope when I move out. my family is all excited for me. They want me to take mycology up as a possible career. Dono what the hell im gonna be doing with mushrooms but o well. maybe this.

Inspiration!!!:mushroom2::rockon:


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Re: Spore microscopy photos [Re: auweia]
    #8088578 - 03/01/08 01:29 AM (15 years, 10 months ago)

Psilocybe cyanescens, Fakhface collection

Spores, 1000x



Spores, 1000x, three spores in the middle have their germ pores facing up.



Spores, 1000x, end view



Spores, 2800x



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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #8122387 - 03/09/08 04:14 AM (15 years, 10 months ago)

Psilocybe villarrealiae spores, 1000x, Prankster239 collection






Psilocybe caerulescens spores, 1000x, Prankster239 collection








The biggest difference I see in the spores between the two species is the color. It is noticeable both looking at the print and through the the microscope. The P. villarrealiae spores are a reddish brown while the P. caerulescens spores are closer to a charcoal gray, just barely if at all brown. After looking at the P. villarrealiae spores in the microscope for awhile, the P. caerulescens looked like chunks of charcoal by comparison.



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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #8380429 - 05/09/08 01:05 AM (15 years, 8 months ago)

Psilocybe ovoideocystidiata (Belmont) - Shroomydan collection

Scanning electron microscopy by Scout24

1800x:


3000x:


10000x


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #8380773 - 05/09/08 04:10 AM (15 years, 8 months ago)

Whooo, neat-o.  Who does Scout know?  :wink:


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Re: Spore microscopy photos [Re: CureCat]
    #9655467 - 01/21/09 10:00 PM (15 years, 10 days ago)

Bluing Galerina, Curecat collection, 1000x:

Copelandia cyanescens, mushroomhunter10 collection

100x:

2000x, ten micron divisions:

Psilocybe guilartensis, BlimeyGrimey collection:

100x:

2000x:



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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9655591 - 01/21/09 10:20 PM (15 years, 10 days ago)

Gymnopilus luteocarneus, Santa Cruz Fungus Fair 2009

Spores moderately roughened, inequilateral, dextrinoid, no germ pore, 4.5 – 5 × 7 – 8 micrometers.



The spores turned from golden yellow to brick red after being in Melzer's reagent for an hour.



Gymnopilus lutescens

Spores roughened, 7 - 8 x 5 micrometers.  These spores are rougher than the spores from G. luteocarneus.





Spores mildly dextrinoid



Gymnopilus flavidellus

Spores 6 – 7 (8) x 4 – 4.5 um, minutely roughened



Spores immediately dextrinoid



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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9662330 - 01/22/09 10:20 PM (15 years, 9 days ago)

I wet myself.  Sweet thread, awesome photos.  I'm jealous. :wink:

Excellent work.  Who identified the Gymnopilus flavidellus?


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Re: Spore microscopy photos [Re: Mr. Mushrooms]
    #9662696 - 01/22/09 11:37 PM (15 years, 9 days ago)

Quote:

Excellent work.  Who identified the Gymnopilus flavidellus?




I wish I knew.  I asked around but no one knew who id'd the gyms.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9662707 - 01/22/09 11:41 PM (15 years, 9 days ago)

Hmmm who was that masked man?  Well, if you checked the monograph as I did (I'm sure you did) you know the microscopy involved.  The species is relatively close to some others.

Very interesting, Holmes.  Wouldn't you say?  :sherlock:


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9708070 - 01/30/09 07:38 PM (15 years, 1 day ago)

Tuber oregonense, Ice House Shaman collection

100x

200x

2000x 10.8 micron divisions

Spores measure 26 - 52 x 24 - 28 micrometers (not counting the ornamentation of course). These are more than twice as big as the largest mushroom spores I have seen!

The spore sacs that have just two spores have much larger spores than the sacs that have 3 or 4 spores. The spores are evenly distributed throughout the inside of the truffle.

Here are some pics of the black truffle spores



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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9732032 - 02/03/09 10:55 PM (14 years, 11 months ago)

Curecat collection, tiny Psilocybe species 12/6/08

Three normal sized spores


The spores are inequilateral, almost diamond shaped in this view



Three more average sized spores



A really big spore!  This one measures 16.21 x 7.28 um



Two larger than average spores



A really big spore next to a normal size spore.  90% of the spores were the smaller size and a few are much larger (maybe from 2 spored basidia?)





Spore measurements:
10.92 x 6.19
11.19 x 6.12
11.81 x 5.89
12.02 x 6.58
12.37 x 6.77
12.51 x 6.03
12.63 x 6.87
12.65 x 5.94
12.66 x 7.17
12.72 x 6.59
13.65 x 6.82
13.74 x 6.58
16.09 x 7.52
16.21 x 7.28


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9805917 - 02/16/09 12:56 AM (14 years, 11 months ago)

Stropharia riparia spores, 100x

2/12/09

Mountain View, CA

Darkfield illumination



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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9805981 - 02/16/09 01:25 AM (14 years, 11 months ago)

Beautiful stuff.  Thanks for sharing. 

Quote:

Spore measurements:
10.92 x 6.19
11.19 x 6.12
11.81 x 5.89
12.02 x 6.58
12.37 x 6.77
12.51 x 6.03
12.63 x 6.87
12.65 x 5.94
12.66 x 7.17
12.72 x 6.59
13.65 x 6.82
13.74 x 6.58
16.09 x 7.52
16.21 x 7.28




Incredibly accurate measurements too.  Nice.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #10373757 - 05/21/09 01:34 AM (14 years, 8 months ago)

Gymnopilus spores, shroomgatherer collection 2/21/09



Psilocybe subcubensis, EQ print



Psilocybe subaeruginascens, coolboarderguy collection



Leptonia incana, rickstar collection



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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #17509707 - 01/06/13 10:10 PM (11 years, 24 days ago)



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Offlinepsylosymonreturns
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #17509980 - 01/06/13 11:10 PM (11 years, 24 days ago)

nice work man. did you ever get a chance to look at those 3 Panaeolous and that Stropharia for me from 2011?


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: psylosymonreturns]
    #17509990 - 01/06/13 11:14 PM (11 years, 24 days ago)

Quote:

psylosymonreturns said:
did you ever get a chance to look at those 3 Panaeolous and that Stropharia for me from 2011?




Yes, they were kind of boring.  I think all your Panaeolus samples are the same.  I took a bunch of pictures but have not uploaded them yet.  I couldn't tell if the spores were smooth or very minutely ornamented, so I guess I should call them smooth.  If that is the case, they would fall into the Panaeolus cinctulus bucket.  I have your samples here and I have much better microscopes now so I might take another look at them soon.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #17510043 - 01/06/13 11:30 PM (11 years, 24 days ago)

wow cinctulus eh! i was thinking one was for sure P olivaceus , another one was a real cool one macroscopicly resembling P fimicola and the last one i now am pretty sure is just P acuminatus. But the spores dont lie i guess ! maybe your new scope will see something different though.

thanks so much for looking at them for me . :thumbup:


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Re: Spore microscopy photos [Re: psylosymonreturns]
    #17867390 - 02/26/13 03:09 AM (10 years, 10 months ago)

Psilocybe allenii 1000x







ITS Sequence: http://www.ncbi.nlm.nih.gov/nuccore/HE994450.1

Spore measurements:

10.9 [11.9 ; 12.3] 13.2 × 6.1 [6.7 ; 6.9] 7.5 µm
Q = 1.6 [1.7 ; 1.8] 2 ; N = 35 ; C = 95%
Me = 12.1 × 6.8 µm ; Qe = 1.8


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #17868271 - 02/26/13 10:19 AM (10 years, 10 months ago)

How are you getting these microscope images? Are you using an electron microscope? Maybe the answer is obvious, but unfortunately I don't have the resources to be very familiar with Electron microscopes...

The images are beautiful btw, can we expect much more microscopy, of this quality, to come?


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Alan Rockefeller said:No!  Do not feed the type collection of a new species to animals!


Edited by BjJiggles (02/26/13 10:21 AM)


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Re: Spore microscopy photos [Re: BjJiggles]
    #17868277 - 02/26/13 10:21 AM (10 years, 10 months ago)



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Re: Spore microscopy photos [Re: The Lightning]
    #17868300 - 02/26/13 10:28 AM (10 years, 10 months ago)

That camera looks awesome, I've been wanting one like that, that will mount in the eyepiece


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Alan Rockefeller said:No!  Do not feed the type collection of a new species to animals!


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Re: Spore microscopy photos [Re: BjJiggles]
    #17869336 - 02/26/13 01:57 PM (10 years, 10 months ago)

Quote:

BjJiggles said:
How are you getting these microscope images? Are you using an electron microscope? Maybe the answer is obvious, but unfortunately I don't have the resources to be very familiar with Electron microscopes...




I used a Nikon Eclipse 80i with DIC prisms and objectives.

Quote:

The images are beautiful btw, can we expect much more microscopy, of this quality, to come?




Occasionally, when I take my samples to a college with a $50,000 scope.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #17869511 - 02/26/13 02:25 PM (10 years, 10 months ago)

That Nikon is a badass scope(read about it). Is that your personal microscope?


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Alan Rockefeller said:No!  Do not feed the type collection of a new species to animals!


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: BjJiggles]
    #17869536 - 02/26/13 02:30 PM (10 years, 10 months ago)

Quote:

BjJiggles said:
Is that your personal microscope?





No.


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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #22665517 - 12/16/15 10:42 PM (8 years, 1 month ago)

Lepiota spheniscispora in Melzers



Pseudobaeospora




Lactarius fragilis var. rubidus



Lepiota castanedisca



Psilocybe cyanescens



Lactarius pallescens




Ramaria formosa




Inocybe kaufmanii http://mushroomobserver.org/122809



Inocybe grammata http://mushroomobserver.org/122814



Psathyrella ulignicicola sensu PNW http://mushroomobserver.org/123283



Tomentella sp.

http://mushroomobserver.org/123177



Cortinarius subgenus Leprocybe



Pholiotina teneroides

http://mushroomobserver.org/127696




Psilocybe muliercula

The first record of this species from Oaxaca

http://mushroomobserver.org/114470



Pholiota sp. from a landslide — in San José Del Pacífico, Oaxaca, Mexico.



Psilocybe subtropicalis

http://mushroomobserver.org/127923 — in Coatepec, Veracruz-Llave, Mexico.



Trichoglossum hirsutum

http://mushroomobserver.org/129406 — at Muir Woods National Monument.



Psilocybe subaeruginascens

http://mushroomobserver.org/128946



An undescribed Otidea species

http://mushroomobserver.org/115972



Pleurotus ostreatus 1000x — at Muir Woods National Monument.



Psilocybe allenii

http://mushroomobserver.org/26833



Psilocybe allenii

http://mushroomobserver.org/58787



Craterellus cornucopioides 1000x — at Muir Woods National Monument.



Craterellus cornucopioides 1000x — at Muir Woods National Monument.




Psilocybe yungensis spores 1000x







Pleurotus ostreatus 400x phase contrast



Psilocybe mexicana spores and cheilocystidia 400x phase contrast — with Pedro Paramo at San Agustin Loxicha, Oaxaca, Mexico.



Psilocybe mexicana spores and cheilocystidia 400x phase contrast — at San Agustin Loxicha, Oaxaca, Mexico.



Psilocybe pelliculosa




Trichoglossum hirsutum

http://mushroomobserver.org/129406 — at Muir Woods National Monument.



Trichoglossum hirsutum

http://mushroomobserver.org/129406 — at Muir Woods National Monument.





Trichoglossum hirsutum

http://mushroomobserver.org/129406 — at Muir Woods National Monument.



Psilocybe cubensis, from a print labeled "Panama"




Gymnopilus, from a print labeled "Gymnopilus aeruginosus"



Psilocybe cyanescens darkfield 200x




Battarrea phalloides?

http://mushroomobserver.org/129748



Trichoglossum hirsutum

http://en.wikipedia.org/wiki/Trichoglossum_hirsutum



Psilocybe allenii cheilocystidia 600x

http://mushroomobserver.org/58787



Psilocybe allenii cheilocystidia 600x

http://mushroomobserver.org/58787



Psilocybe allenii cheilocystidia 600x

http://mushroomobserver.org/58787



Psilocybe allenii cheilocystidia 600x

http://mushroomobserver.org/58787



Psilocybe allenii cheilocystidia darkfield 600x

http://mushroomobserver.org/58787




Psilocybe subaeruginosa cheilocystidia 600x

http://mushroomobserver.org/88182




Psilocybe cyanescens spores 1000x

http://mushroomobserver.org/119071



Psilocybe subaeruginosa spores 1000x

http://mushroomobserver.org/88182




Psilocybe baeocystis spores 1000x

http://mushroomobserver.org/130071



Gymnopilus luteofolius gill crush mount




Psilocybe quebecensis spores and cheilocystidia 1000x




Psilocybe quebecensis pleurocystidia 600x




Psilocybe quebecensis cheilocystidia 600x




Psilocybe baeocystis spores 1000x

http://mushroomobserver.org/130071




Tulostoma clathrosporum

http://mushroomobserver.org/129972



Panaeolus olivaceus 1000x

http://mushroomobserver.org/129971



Panaeolus olivaceus 1000x

http://mushroomobserver.org/129971



Asterophora lycoperdoides 1000x

http://mushroomobserver.org/127934




Asterophora lycoperdoides 1000x

http://mushroomobserver.org/127934



Tuber gibbosum

http://mushroomobserver.org/130478 — in Portland, Oregon.



Amanita section Vaginatae
http://mushroomobserver.org/143883



Crepidotus

http://mushroomobserver.org/143631





A new species of Amanita from section Lepidella
http://mushroomobserver.org/143625



Entoloma



Cronartium quercuum

http://mushroomobserver.org/143627



Psilocybe subtropicalis 1000x

http://mushroomobserver.org/210888



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InvisibleLhun
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #22665573 - 12/16/15 10:57 PM (8 years, 1 month ago)

Wow, epic update to an old classic thread.  Good stuff Alan!

:dawerp:


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