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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: auweia]
    #8088578 - 03/01/08 01:29 AM (15 years, 10 months ago)

Psilocybe cyanescens, Fakhface collection

Spores, 1000x



Spores, 1000x, three spores in the middle have their germ pores facing up.



Spores, 1000x, end view



Spores, 2800x



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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #8122387 - 03/09/08 04:14 AM (15 years, 10 months ago)

Psilocybe villarrealiae spores, 1000x, Prankster239 collection






Psilocybe caerulescens spores, 1000x, Prankster239 collection








The biggest difference I see in the spores between the two species is the color. It is noticeable both looking at the print and through the the microscope. The P. villarrealiae spores are a reddish brown while the P. caerulescens spores are closer to a charcoal gray, just barely if at all brown. After looking at the P. villarrealiae spores in the microscope for awhile, the P. caerulescens looked like chunks of charcoal by comparison.



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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #8380429 - 05/09/08 01:05 AM (15 years, 8 months ago)

Psilocybe ovoideocystidiata (Belmont) - Shroomydan collection

Scanning electron microscopy by Scout24

1800x:


3000x:


10000x


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InvisibleCureCat
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #8380773 - 05/09/08 04:10 AM (15 years, 8 months ago)

Whooo, neat-o.  Who does Scout know?  :wink:


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: CureCat]
    #9655467 - 01/21/09 10:00 PM (15 years, 10 days ago)

Bluing Galerina, Curecat collection, 1000x:

Copelandia cyanescens, mushroomhunter10 collection

100x:

2000x, ten micron divisions:

Psilocybe guilartensis, BlimeyGrimey collection:

100x:

2000x:



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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9655591 - 01/21/09 10:20 PM (15 years, 10 days ago)

Gymnopilus luteocarneus, Santa Cruz Fungus Fair 2009

Spores moderately roughened, inequilateral, dextrinoid, no germ pore, 4.5 – 5 × 7 – 8 micrometers.



The spores turned from golden yellow to brick red after being in Melzer's reagent for an hour.



Gymnopilus lutescens

Spores roughened, 7 - 8 x 5 micrometers.  These spores are rougher than the spores from G. luteocarneus.





Spores mildly dextrinoid



Gymnopilus flavidellus

Spores 6 – 7 (8) x 4 – 4.5 um, minutely roughened



Spores immediately dextrinoid



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InvisibleMr. Mushrooms
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9662330 - 01/22/09 10:20 PM (15 years, 9 days ago)

I wet myself.  Sweet thread, awesome photos.  I'm jealous. :wink:

Excellent work.  Who identified the Gymnopilus flavidellus?


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Mr. Mushrooms]
    #9662696 - 01/22/09 11:37 PM (15 years, 9 days ago)

Quote:

Excellent work.  Who identified the Gymnopilus flavidellus?




I wish I knew.  I asked around but no one knew who id'd the gyms.


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InvisibleMr. Mushrooms
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9662707 - 01/22/09 11:41 PM (15 years, 9 days ago)

Hmmm who was that masked man?  Well, if you checked the monograph as I did (I'm sure you did) you know the microscopy involved.  The species is relatively close to some others.

Very interesting, Holmes.  Wouldn't you say?  :sherlock:


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9708070 - 01/30/09 07:38 PM (15 years, 1 day ago)

Tuber oregonense, Ice House Shaman collection

100x

200x

2000x 10.8 micron divisions

Spores measure 26 - 52 x 24 - 28 micrometers (not counting the ornamentation of course). These are more than twice as big as the largest mushroom spores I have seen!

The spore sacs that have just two spores have much larger spores than the sacs that have 3 or 4 spores. The spores are evenly distributed throughout the inside of the truffle.

Here are some pics of the black truffle spores



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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9732032 - 02/03/09 10:55 PM (14 years, 11 months ago)

Curecat collection, tiny Psilocybe species 12/6/08

Three normal sized spores


The spores are inequilateral, almost diamond shaped in this view



Three more average sized spores



A really big spore!  This one measures 16.21 x 7.28 um



Two larger than average spores



A really big spore next to a normal size spore.  90% of the spores were the smaller size and a few are much larger (maybe from 2 spored basidia?)





Spore measurements:
10.92 x 6.19
11.19 x 6.12
11.81 x 5.89
12.02 x 6.58
12.37 x 6.77
12.51 x 6.03
12.63 x 6.87
12.65 x 5.94
12.66 x 7.17
12.72 x 6.59
13.65 x 6.82
13.74 x 6.58
16.09 x 7.52
16.21 x 7.28


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9805917 - 02/16/09 12:56 AM (14 years, 11 months ago)

Stropharia riparia spores, 100x

2/12/09

Mountain View, CA

Darkfield illumination



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InvisibleMr. Mushrooms
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #9805981 - 02/16/09 01:25 AM (14 years, 11 months ago)

Beautiful stuff.  Thanks for sharing. 

Quote:

Spore measurements:
10.92 x 6.19
11.19 x 6.12
11.81 x 5.89
12.02 x 6.58
12.37 x 6.77
12.51 x 6.03
12.63 x 6.87
12.65 x 5.94
12.66 x 7.17
12.72 x 6.59
13.65 x 6.82
13.74 x 6.58
16.09 x 7.52
16.21 x 7.28




Incredibly accurate measurements too.  Nice.


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #10373757 - 05/21/09 01:34 AM (14 years, 8 months ago)

Gymnopilus spores, shroomgatherer collection 2/21/09



Psilocybe subcubensis, EQ print



Psilocybe subaeruginascens, coolboarderguy collection



Leptonia incana, rickstar collection



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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #17509707 - 01/06/13 10:10 PM (11 years, 24 days ago)



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Offlinepsylosymonreturns
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #17509980 - 01/06/13 11:10 PM (11 years, 24 days ago)

nice work man. did you ever get a chance to look at those 3 Panaeolous and that Stropharia for me from 2011?


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: psylosymonreturns]
    #17509990 - 01/06/13 11:14 PM (11 years, 24 days ago)

Quote:

psylosymonreturns said:
did you ever get a chance to look at those 3 Panaeolous and that Stropharia for me from 2011?




Yes, they were kind of boring.  I think all your Panaeolus samples are the same.  I took a bunch of pictures but have not uploaded them yet.  I couldn't tell if the spores were smooth or very minutely ornamented, so I guess I should call them smooth.  If that is the case, they would fall into the Panaeolus cinctulus bucket.  I have your samples here and I have much better microscopes now so I might take another look at them soon.


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Offlinepsylosymonreturns
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #17510043 - 01/06/13 11:30 PM (11 years, 24 days ago)

wow cinctulus eh! i was thinking one was for sure P olivaceus , another one was a real cool one macroscopicly resembling P fimicola and the last one i now am pretty sure is just P acuminatus. But the spores dont lie i guess ! maybe your new scope will see something different though.

thanks so much for looking at them for me . :thumbup:


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OfflineAlan RockefellerM
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Re: Spore microscopy photos [Re: psylosymonreturns]
    #17867390 - 02/26/13 03:09 AM (10 years, 10 months ago)

Psilocybe allenii 1000x







ITS Sequence: http://www.ncbi.nlm.nih.gov/nuccore/HE994450.1

Spore measurements:

10.9 [11.9 ; 12.3] 13.2 × 6.1 [6.7 ; 6.9] 7.5 µm
Q = 1.6 [1.7 ; 1.8] 2 ; N = 35 ; C = 95%
Me = 12.1 × 6.8 µm ; Qe = 1.8


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OfflineBjJiggles
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Re: Spore microscopy photos [Re: Alan Rockefeller]
    #17868271 - 02/26/13 10:19 AM (10 years, 10 months ago)

How are you getting these microscope images? Are you using an electron microscope? Maybe the answer is obvious, but unfortunately I don't have the resources to be very familiar with Electron microscopes...

The images are beautiful btw, can we expect much more microscopy, of this quality, to come?


--------------------

Alan Rockefeller said:No!  Do not feed the type collection of a new species to animals!


Edited by BjJiggles (02/26/13 10:21 AM)


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