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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Re: Spore microscopy photos [Re: auweia]
#8088578 - 03/01/08 01:29 AM (15 years, 10 months ago) |
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Psilocybe cyanescens, Fakhface collection
Spores, 1000x

Spores, 1000x, three spores in the middle have their germ pores facing up.

Spores, 1000x, end view

Spores, 2800x
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Psilocybe villarrealiae spores, 1000x, Prankster239 collection


Psilocybe caerulescens spores, 1000x, Prankster239 collection



The biggest difference I see in the spores between the two species is the color. It is noticeable both looking at the print and through the the microscope. The P. villarrealiae spores are a reddish brown while the P. caerulescens spores are closer to a charcoal gray, just barely if at all brown. After looking at the P. villarrealiae spores in the microscope for awhile, the P. caerulescens looked like chunks of charcoal by comparison.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Psilocybe ovoideocystidiata (Belmont) - Shroomydan collection
Scanning electron microscopy by Scout24
1800x:

3000x:

10000x
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CureCat
Strangest


Registered: 04/19/06
Posts: 14,058
Loc: clawing your furniture
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Whooo, neat-o. Who does Scout know?
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Re: Spore microscopy photos [Re: CureCat]
#9655467 - 01/21/09 10:00 PM (15 years, 10 days ago) |
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Bluing Galerina, Curecat collection, 1000x:


Copelandia cyanescens, mushroomhunter10 collection
100x:

2000x, ten micron divisions:



Psilocybe guilartensis, BlimeyGrimey collection:
100x:

2000x:



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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Gymnopilus luteocarneus, Santa Cruz Fungus Fair 2009
Spores moderately roughened, inequilateral, dextrinoid, no germ pore, 4.5 – 5 × 7 – 8 micrometers.

The spores turned from golden yellow to brick red after being in Melzer's reagent for an hour.

Gymnopilus lutescens
Spores roughened, 7 - 8 x 5 micrometers. These spores are rougher than the spores from G. luteocarneus.


Spores mildly dextrinoid

Gymnopilus flavidellus
Spores 6 – 7 (8) x 4 – 4.5 um, minutely roughened

Spores immediately dextrinoid
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Mr. Mushrooms
Spore Print Collector


Registered: 05/25/08
Posts: 13,018
Loc: Registered: 6/04/02
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I wet myself. Sweet thread, awesome photos. I'm jealous. 
Excellent work. Who identified the Gymnopilus flavidellus?
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Quote:
Excellent work. Who identified the Gymnopilus flavidellus?
I wish I knew. I asked around but no one knew who id'd the gyms.
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Mr. Mushrooms
Spore Print Collector


Registered: 05/25/08
Posts: 13,018
Loc: Registered: 6/04/02
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Hmmm who was that masked man? Well, if you checked the monograph as I did (I'm sure you did) you know the microscopy involved. The species is relatively close to some others.
Very interesting, Holmes. Wouldn't you say?
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Tuber oregonense, Ice House Shaman collection
100x

200x



2000x 10.8 micron divisions

Spores measure 26 - 52 x 24 - 28 micrometers (not counting the ornamentation of course). These are more than twice as big as the largest mushroom spores I have seen!
The spore sacs that have just two spores have much larger spores than the sacs that have 3 or 4 spores. The spores are evenly distributed throughout the inside of the truffle.
Here are some pics of the black truffle spores




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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Curecat collection, tiny Psilocybe species 12/6/08
Three normal sized spores

The spores are inequilateral, almost diamond shaped in this view

Three more average sized spores

A really big spore! This one measures 16.21 x 7.28 um

Two larger than average spores

A really big spore next to a normal size spore. 90% of the spores were the smaller size and a few are much larger (maybe from 2 spored basidia?)


Spore measurements: 10.92 x 6.19 11.19 x 6.12 11.81 x 5.89 12.02 x 6.58 12.37 x 6.77 12.51 x 6.03 12.63 x 6.87 12.65 x 5.94 12.66 x 7.17 12.72 x 6.59 13.65 x 6.82 13.74 x 6.58 16.09 x 7.52 16.21 x 7.28
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Stropharia riparia spores, 100x
2/12/09
Mountain View, CA
Darkfield illumination
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Mr. Mushrooms
Spore Print Collector


Registered: 05/25/08
Posts: 13,018
Loc: Registered: 6/04/02
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Beautiful stuff. Thanks for sharing.
Quote:
Spore measurements: 10.92 x 6.19 11.19 x 6.12 11.81 x 5.89 12.02 x 6.58 12.37 x 6.77 12.51 x 6.03 12.63 x 6.87 12.65 x 5.94 12.66 x 7.17 12.72 x 6.59 13.65 x 6.82 13.74 x 6.58 16.09 x 7.52 16.21 x 7.28
Incredibly accurate measurements too. Nice.
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Alan Rockefeller
Mycologist


Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Gymnopilus spores, shroomgatherer collection 2/21/09

Psilocybe subcubensis, EQ print

Psilocybe subaeruginascens, coolboarderguy collection

Leptonia incana, rickstar collection
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 5 months
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nice work man. did you ever get a chance to look at those 3 Panaeolous and that Stropharia for me from 2011?
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Quote:
psylosymonreturns said: did you ever get a chance to look at those 3 Panaeolous and that Stropharia for me from 2011?
Yes, they were kind of boring. I think all your Panaeolus samples are the same. I took a bunch of pictures but have not uploaded them yet. I couldn't tell if the spores were smooth or very minutely ornamented, so I guess I should call them smooth. If that is the case, they would fall into the Panaeolus cinctulus bucket. I have your samples here and I have much better microscopes now so I might take another look at them soon.
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 5 months
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wow cinctulus eh! i was thinking one was for sure P olivaceus , another one was a real cool one macroscopicly resembling P fimicola and the last one i now am pretty sure is just P acuminatus. But the spores dont lie i guess ! maybe your new scope will see something different though.
thanks so much for looking at them for me .
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,276
Last seen: 17 minutes, 28 seconds
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Psilocybe allenii 1000x



ITS Sequence: http://www.ncbi.nlm.nih.gov/nuccore/HE994450.1
Spore measurements:
10.9 [11.9 ; 12.3] 13.2 × 6.1 [6.7 ; 6.9] 7.5 µm Q = 1.6 [1.7 ; 1.8] 2 ; N = 35 ; C = 95% Me = 12.1 × 6.8 µm ; Qe = 1.8
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BjJiggles
Gym seeker



Registered: 03/13/12
Posts: 2,736
Loc:
Last seen: 8 years, 25 days
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How are you getting these microscope images? Are you using an electron microscope? Maybe the answer is obvious, but unfortunately I don't have the resources to be very familiar with Electron microscopes...
The images are beautiful btw, can we expect much more microscopy, of this quality, to come?
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 Alan Rockefeller said:No! Do not feed the type collection of a new species to animals!
Edited by BjJiggles (02/26/13 10:21 AM)
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