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InvisibleDontPlay
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Registered: 12/13/07
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Jar inoculation
    #7758976 - 12/14/07 04:01 PM (16 years, 1 month ago)

Could you use a fully colonized cake and (in a glovebox )scrape a little into a jar of grain. Would it colonize ? If so how much would it take ?

Only asking because im out of spores and down to a few cakes on my first grow.


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InvisibleKillerPicklez
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Re: Jar inoculation [Re: DontPlay]
    #7759021 - 12/14/07 04:13 PM (16 years, 1 month ago)

Quote:

DontPlay said:
Could you use a fully colonized cake and (in a glovebox )scrape a little into a jar of grain. Would it colonize ? If so how much would it take ?

Only asking because im out of spores and down to a few cakes on my first grow.




Yes, This is a technique known as spawning. Atleast on a small scale. Youd have to sterilize your grain as well. but id use maybe a 1/4 of your cake to do it.


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Offlinedrwatson
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Re: Jar inoculation [Re: DontPlay]
    #7759032 - 12/14/07 04:15 PM (16 years, 1 month ago)

well you could do that or you could take your old syringe, fill it with spring water, shoot that water into your colonized cake and then suck the water back up into the syringe.

You won't get all the water back but what you do get back will be chalk full of living tissues. Then you've got a full syringe again!

No one is answering you because this has been discussed in detail a few times. use the search for more details.


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InvisibleDontPlay
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Re: Jar inoculation [Re: drwatson]
    #7759098 - 12/14/07 04:30 PM (16 years, 1 month ago)

I'm sorry did look around alot, the majority of what I saw was Grain to Grain tutorials. But that was all I needed to know, thanks for the help.


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OfflineNibin
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Re: Jar inoculation [Re: KillerPicklez]
    #7759574 - 12/14/07 06:33 PM (16 years, 1 month ago)

Quote:

KillerPicklez said:
Quote:

DontPlay said:
Could you use a fully colonized cake and (in a glovebox )scrape a little into a jar of grain. Would it colonize ? If so how much would it take ?

Only asking because im out of spores and down to a few cakes on my first grow.




Yes, This is a technique known as spawning. Atleast on a small scale. Youd have to sterilize your grain as well. but id use maybe a 1/4 of your cake to do it.




It isn't insomuch spawning as just a grain to gran transfer but made with cakes.

The second system, with the water, works ok with a cake if it hasn't got the dry verm barrier (using micropore over the holes instead). If you used the barrier you would be better off making your new jars and once they are ready, break a cake in half and transfer some of the mycelium from inside into the grain jar. Or make pf cakes with the micropore and no verm layer and transfer to those instead.


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OfflinePentahedral
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Re: Jar inoculation [Re: Nibin]
    #7759982 - 12/14/07 08:14 PM (16 years, 1 month ago)

Kinda of off topic, but what's the most accepted way to make a mycelium syringe? I've got a fully colonized PF BRF+Verm layer 1/2 pint jar and was planning on making ~10 syringes from it to inoculate popcorn.

Is it best to cut some out and put the piece of mycelium into sterile water, or the other way that was previously mentioned that you just squirt water into the jar?


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OfflineNibin
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Re: Jar inoculation [Re: Pentahedral]
    #7760005 - 12/14/07 08:19 PM (16 years, 1 month ago)

If you have a dry verm barrier, by injecting water into the jar you are risking picking up contaminant spores that were in the verm barrier when you suck up the water. I'd use a piece from the centre of a cake.


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OfflinePentahedral
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Re: Jar inoculation [Re: Nibin]
    #7760021 - 12/14/07 08:23 PM (16 years, 1 month ago)

Quote:

Nibin said:
If you have a dry verm barrier, by injecting water into the jar you are risking picking up contaminant spores that were in the verm barrier when you suck up the water. I'd use a piece from the centre of a cake.




Now I have a glovebox, so I'll do it in there. Hopefully tomorrow I'll have 9 3ml syringes for the 12 pints of popcorn!


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OfflineNibin
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Re: Jar inoculation [Re: Pentahedral]
    #7760046 - 12/14/07 08:32 PM (16 years, 1 month ago)

I'm talking about spores that fell into the jars through the holes during colonisation, not during the time you put the needle in to add water.

That dry verm barrier will have 2-3 weeks worth of crap on it.


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Invisiblethedefone
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Re: Jar inoculation [Re: Nibin]
    #7760108 - 12/14/07 08:54 PM (16 years, 1 month ago)



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