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tony8404
Stranger


Registered: 06/14/05
Posts: 1,323
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Pc with Lc question and a spore syringe q all together :O)
#7750361 - 12/12/07 05:38 PM (16 years, 1 month ago) |
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my last two tries at a new lc have failed, due to carmilization... maybe i am not pcing correctly...
first, i am using a small pc 4.5 quart for my lc. my lc is a baby bottle small baby bottle.... so i use 1oo ml of distilled water and use 3ml of karo clear syrup... i place the bottle in the microwave to get the water hot then i add the karo. after that i place nipple on and shake it up for a bit. then i add water to the pc, how much water do u use?? do u fill water up the side of the baby bottle or just enough water that the bottle doesnt sit in it? so far i have placed water in the pc to wear it just is touching the bottle like maybe half inch up... this time i was gonna try enough water to cover the insert peice....
also, last question is with a syringe thats been in a refrigerator for months, do i have to do anything to it before i use it for inoculation???? like do i have to let it warm up or shake it for a while???? cause i see no spore clumps...out of the 5 i ordered only one was i able to spot spores.. thanks guys
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tony8404
Stranger


Registered: 06/14/05
Posts: 1,323
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Re: Pc with Lc question and a spore syringe q all together :O) [Re: tony8404]
#7750469 - 12/12/07 06:00 PM (16 years, 1 month ago) |
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please help .... i would like to do this lc tonight thanks
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Molasses
sobriety with aside of what thefuck am i doing?



Registered: 11/03/07
Posts: 564
Loc: Marijuanaville, Vermont
Last seen: 11 years, 5 months
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Re: Pc with Lc question and a spore syringe q all together :O) [Re: tony8404]
#7751025 - 12/12/07 07:43 PM (16 years, 1 month ago) |
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its been 20 minutes dude, pop a Valium...
Just use hot water from the tap, 4ml karo to 100ml water
pc for 20-25 minutes, with the water at a level it won't alter the contents of the jar, but enough that it won't burn dry in the pc, an inch or two should be good
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Vyrk
Stranger

Registered: 12/12/07
Posts: 70
Last seen: 15 years, 5 months
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Re: PC with Lc question and a spore syringe q all together :O) [Re: Molasses]
#7751047 - 12/12/07 07:50 PM (16 years, 1 month ago) |
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see that the % for the LC cultures is based on the weight, not the volume.
And the syrup doesn`t has the same density that the water.
And for the syringe, you shouldn't`t have any problem, its the same if you incubate after inoculation.
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Molasses
sobriety with aside of what thefuck am i doing?



Registered: 11/03/07
Posts: 564
Loc: Marijuanaville, Vermont
Last seen: 11 years, 5 months
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Re: PC with Lc question and a spore syringe q all together :O) [Re: Vyrk]
#7751058 - 12/12/07 07:53 PM (16 years, 1 month ago) |
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good point vyrk, i weigh mine out on a scale, with 4 grams of karo and 96-100 grams water
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Vyrk
Stranger

Registered: 12/12/07
Posts: 70
Last seen: 15 years, 5 months
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Re: PC with Lc question and a spore syringe q all together :O) [Re: Molasses]
#7751066 - 12/12/07 07:55 PM (16 years, 1 month ago) |
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Also, i recomend you another tek... If you wanna try it, is based in a potatoes soup...
I will see if someone post this tek in the forum... if no one do it, i will post it.
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FooMan




Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Pc with Lc question and a spore syringe q all together :O) [Re: tony8404]
#7751075 - 12/12/07 07:58 PM (16 years, 1 month ago) |
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You only need to PC for about 12-15 minutes max IME. Being that what you are sterilizing is completely liquid, it reaches sterilization temperatures much faster than solid substrates, so it doesn't need a very long PC time. This will help stop carmelization.
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Quick WBS Prep
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