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Invisibletony8404
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Pc with Lc question and a spore syringe q all together :O)
    #7750361 - 12/12/07 05:38 PM (16 years, 1 month ago)

my last two tries at a new lc have failed, due to carmilization... maybe i am not pcing correctly...

first, i am using a small pc 4.5 quart for my lc. my lc is a baby bottle small baby bottle.... so i use 1oo ml of distilled water and use 3ml of karo clear syrup... i place the bottle in the microwave to get the water hot then i add the karo. after that i place nipple on and shake it up for a bit. then i add water to the pc, how much water do u use?? do u fill water up the side of the baby bottle or just enough water that the bottle doesnt sit in it? so far i have placed water in the pc to wear it just is touching the bottle like maybe half inch up... this time i was gonna try enough water to cover the insert peice....

also, last question is with a syringe thats been in a refrigerator for months, do i have to do anything to it before i use it for inoculation???? like do i have to let it warm up or shake it for a while???? cause i see no spore clumps...out of the 5 i ordered only one was i able to spot spores.. thanks guys


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Invisibletony8404
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Re: Pc with Lc question and a spore syringe q all together :O) [Re: tony8404]
    #7750469 - 12/12/07 06:00 PM (16 years, 1 month ago)

please help .... i would like to do this lc tonight thanks


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OfflineMolasses
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Re: Pc with Lc question and a spore syringe q all together :O) [Re: tony8404]
    #7751025 - 12/12/07 07:43 PM (16 years, 1 month ago)

its been 20 minutes dude, pop a Valium...

Just use hot water from the tap, 4ml karo to 100ml water

pc for 20-25 minutes, with the water at a level it won't alter the contents of the jar, but enough that it won't burn dry in the pc, an inch or two should be good


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OfflineVyrk
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Re: PC with Lc question and a spore syringe q all together :O) [Re: Molasses]
    #7751047 - 12/12/07 07:50 PM (16 years, 1 month ago)

see that the % for the LC cultures is based on the weight, not the volume.

And the syrup doesn`t has the same density that the water.

And for the syringe, you shouldn't`t have any problem, its the same if you incubate after inoculation.

:stoned:


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OfflineMolasses
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Re: PC with Lc question and a spore syringe q all together :O) [Re: Vyrk]
    #7751058 - 12/12/07 07:53 PM (16 years, 1 month ago)

good point vyrk, i weigh mine out on a scale, with 4 grams of karo and 96-100 grams water


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OfflineVyrk
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Re: PC with Lc question and a spore syringe q all together :O) [Re: Molasses]
    #7751066 - 12/12/07 07:55 PM (16 years, 1 month ago)

Also, i recomend you another tek... If you wanna try it, is based in a potatoes soup...

I will see if someone post this tek in the forum... if no one do it, i will post it.


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InvisibleFooMan
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Re: Pc with Lc question and a spore syringe q all together :O) [Re: tony8404]
    #7751075 - 12/12/07 07:58 PM (16 years, 1 month ago)

You only need to PC for about 12-15 minutes max IME. Being that what you are sterilizing is completely liquid, it reaches sterilization temperatures much faster than solid substrates, so it doesn't need a very long PC time. This will help stop carmelization.


--------------------

Quick WBS Prep


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