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ShRm22
Shroom Lover


Registered: 03/08/06
Posts: 121
Last seen: 15 years, 5 months
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Print to syringe
#7748346 - 12/12/07 08:27 AM (16 years, 1 month ago) |
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Now that in my first grow I have pins on my cakes I am starting to look into spore printing and making syringes. I have read the TEKs and they say to dry out the prints. I was planning on just making a print and then putting it right into a syringe. Would this work or should I just keep a print until I am in need of making a syringe? I have 6 strains that I am planning on rotating by growing two at a time. So about every 3-4 weeks , a new set of two strain syringes are used. If I just made the print right into a syringe and put it into the fridge, would it work with my plan?
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Fahkface
Over-Fiend



Registered: 12/11/06
Posts: 4,821
Loc: In your Mind, Pedro! In y...
Last seen: 13 years, 4 months
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Re: Print to syringe [Re: ShRm22]
#7748393 - 12/12/07 08:42 AM (16 years, 1 month ago) |
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That would just waste space in your fridge. From what I know prints keep being viable for a longer time and therefore I'd suggest to store the spores in print form. And imagine some kind of mold spores or bacteria flies around the area you prepare the syringes in. It wouldn't be so cool if EVERY syringe you made is contaminated because you made them all at the same time etc. The risk of this happening is much bigger when you do it in one run. One more thing to the space issue: One normal print is good for about 6 syringes and even if you'd just take one print of each strain, you'd have 36 syringes blocking your fridge, when you're not planning on wasting any spores.
Again, I'd say: keep the prints and prepare syringes when necessary!
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ShRm22
Shroom Lover


Registered: 03/08/06
Posts: 121
Last seen: 15 years, 5 months
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Re: Print to syringe [Re: Fahkface]
#7748403 - 12/12/07 08:46 AM (16 years, 1 month ago) |
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I am only planning on making 2 syringes per strain. I only have a limited amount of syringes. Would I store the prints at room temp., the fridge, else where? Also, would putting and entire print into one syringe speed up colonization times?
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Fahkface
Over-Fiend



Registered: 12/11/06
Posts: 4,821
Loc: In your Mind, Pedro! In y...
Last seen: 13 years, 4 months
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Re: Print to syringe [Re: ShRm22]
#7748435 - 12/12/07 09:02 AM (16 years, 1 month ago) |
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No you wouldn't want to put a whole print into a syringe. That would pretty much prevent the myc from growing, rather than it would speed things up!
You're doing the right thing to wait other to answer your question as well, for I'm surely not THE one to listen to, though I think my arguments speak for themselves...
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ShRm22
Shroom Lover


Registered: 03/08/06
Posts: 121
Last seen: 15 years, 5 months
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Re: Print to syringe [Re: Fahkface]
#7748521 - 12/12/07 09:48 AM (16 years, 1 month ago) |
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I am planning on making the print on tin foil...after the print is taken, do I need to place in a desiccant chamber to dry or can I just leave in my glove box to dry? Also, to seal the print, could I just simply wrap it with saran rap or would the print get messed up?
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Fahkface
Over-Fiend



Registered: 12/11/06
Posts: 4,821
Loc: In your Mind, Pedro! In y...
Last seen: 13 years, 4 months
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Re: Print to syringe [Re: ShRm22]
#7748627 - 12/12/07 10:30 AM (16 years, 1 month ago) |
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What you should do, it to take the print the way you want and then to cut out another peace of tinfoil that about 2.5 times as long as the peace the print is on (cut it into a square for better handling!) and about 1.5 as wide. Now, fold the long side in the middle, put your print in it and then just seal it by crumpling all open ends (it should be three by then ) tightly.
From my experience you don't need to dry the print. Just let it in the glove box for five minutes and then wrap it up. Always worked for me and so far no one has ever complained about contams using my prints :P
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Print to syringe [Re: Fahkface]
#7749821 - 12/12/07 03:20 PM (16 years, 1 month ago) |
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If you want to make the syringes straight away don't print to foil.
Just sterilize an empty jar in the oven, put the cap in and let it print so you end up with a print on the glass. Take the cap off after a day and inject sterile water into the jar through a micropore covered hole in the lid, swirl about and suck the water back up.
Hey presto, spore syringe.
-------------------- Newcomers guide-----> For all things shroomy
Edited by Nibin (12/12/07 03:22 PM)
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usg543
◕‿◕


Registered: 02/11/07
Posts: 5,192
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Re: Print to syringe [Re: Nibin]
#7749882 - 12/12/07 03:36 PM (16 years, 1 month ago) |
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Quote:
agar said: Nothing NEW here. Just advise for new hands, who don't know.
Ball has a 250 ml SQUAT wide mouth canning jar out.
  
  
 
 
Simply drill a hole in the lid & install an injection port.
If you don't have an injection port. Drill an 1/8th inch hole in the lid. Scuff the surface, around the hole. (both sides, so sealant sticks better)
Dab on some RTV silicone high temp gasket sealer over the hole. (both sides). Allow to cure at least overnight.
PC jar 20 minutes @ 15 psi, with lid LOOSE. (sterilizes it) Remove from PC hot & tighten lid.
In a glove box, or under a flow hood. Cleanly severe a a mushroom cap, place in print jar. Leave lid on 3/4 turn LOOSE (gas exchange - so you don't get condensation from a wet cap).
Allow print to set 24 (+/-)hours. In glove box, carefully remove cap & replace lid. Leave lid 3/4 turn LOOSE for another 24 hours (place in mild indirect sunlight, so the print dries).
Tighten lid TIGHT.
Knock/rap jar on semi/hard surface to dislodge spores.
With a large capacity syringe. (or whatever syringe size you have)
 Inject 125 / 200 ml of STERILE water. Shake jar violently.
Hint - put a few drops of Jet Dry in the STERILE water.
Hint - to make STERILE WATER. simply PC another jar (as above). With STERILE water & Jet Dry solution in it. (Jet Dry serves as an anti-clumping agent) (hint - distilled water works best)
There you have it. Enough spore solution to fill a dozen + 10 ml syringes.
For those with an ultrasonic cleaner.
 Before making new spore syringes. Place sealed jar in it, for an hour. (disperses spores PERFECTLY, so all syringes are fairly equal)
Hint, once print jar is almost EMPTY. Inject 150 ml of sterilized nutrient solution, to make LC.  ~~~~~~~~~~~~~~~~ EDIT TO ADD A VARIANT GAS EXCHANGE METHOD
 
For better gas exchange. Cut a small piece of tyvek to cover the lid. Place tyvek over the jar mouth. Screw lid on TIGHT, to form the tyvek to lid shape. Use a razor, or Exacto knife to trim off excess tyvek.
PC the jar, with the tyvek lid in place. In glove box, cleanly sever mushroom cap. Remove jar ring & tyvek. Place mushroom cap in jar. Replace tyvek & jar ring. Screw ring on TIGHT.
Allow 24-36 hours for print to set thickly. Remove cap, replace tyvek & jar ring, screw ring on tightly. Allow 24 hours for print to DRY.
Remove tyvek, replace with sanitized injection port lid.

The object of this variant is: 1 > to allow for optimal gas exchange. 2 > So, the cap doesn't begin to rot. 3 > so the print dries quickly.
EDIT (again)
Do not try to pound big caps into little jars.

http://www.shroomery.org/forums/showflat.php/Number/5276177#5276177
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c0_hush
Stranger



Registered: 07/16/06
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Re: Print to syringe [Re: usg543]
#7749900 - 12/12/07 03:43 PM (16 years, 1 month ago) |
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Holy shit, that little jar rules! I should look into getting a bunch of these in the near future, now that I'm also getting some pin development... Thx for the tek btw.
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Print to syringe [Re: c0_hush]
#7749938 - 12/12/07 03:56 PM (16 years, 1 month ago) |
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Great follow through with images usg543. Couldn't be bothered to search for that post.
Happy birthday btw
-------------------- Newcomers guide-----> For all things shroomy
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