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Funghetto
Little Mushroom



Registered: 11/12/07
Posts: 44
Loc: Europe
Last seen: 15 years, 10 months
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Spore Print VS Syringe
#7748022 - 12/12/07 04:22 AM (16 years, 1 month ago) |
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I have a spore print from which I can get 8 syringes but now for my inoculation I need only one. Yet every time I make a syringe from a portion of spore print there is a risk of contamination. Here is my question:
What is better?
1. Make only one syringe and conserve the spore print. 2. Make 8 syringes, use one and conserve the others.
How and how long can I conserve syringes?
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StanleyPain
Supporter



Registered: 07/30/05
Posts: 63
Last seen: 11 years, 3 months
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Re: Spore Print VS Syringe [Re: Funghetto]
#7748041 - 12/12/07 04:47 AM (16 years, 1 month ago) |
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If your methods of transfer from spore print to syringe are proven to be contaminant free, then go ahead with the transfer. Otherwise I would recommend a test of the sterility conditions before you risk losing a print.
-------------------- -= StanleyPain
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Spore Print VS Syringe [Re: StanleyPain]
#7748236 - 12/12/07 07:38 AM (16 years, 1 month ago) |
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Make all syringes at once, and in a sterile environment. Test each them on a single jar to make sure they are not contaminated.
-------------------- Newcomers guide-----> For all things shroomy
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Funghetto
Little Mushroom



Registered: 11/12/07
Posts: 44
Loc: Europe
Last seen: 15 years, 10 months
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Re: Spore Print VS Syringe [Re: Nibin]
#7748553 - 12/12/07 10:01 AM (16 years, 1 month ago) |
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OK, but how can I conserve syringes to protect them from contamination? And how long?
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ShRm22
Shroom Lover


Registered: 03/08/06
Posts: 121
Last seen: 15 years, 5 months
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Re: Spore Print VS Syringe [Re: Funghetto]
#7748567 - 12/12/07 10:06 AM (16 years, 1 month ago) |
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When you say "test" them, just innoc. a little from each syringe into the jar?
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Spore Print VS Syringe [Re: ShRm22]
#7748705 - 12/12/07 10:51 AM (16 years, 1 month ago) |
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only make what you will use!!! The print will not contam setting in a sealed bag and there are only so many contams that you can introduce while making a syringe.
Lets say you make a syringe and you get 5 mold spores in there. You use that right away and maybe one injection site goes bad. Pretty good success.
Now you make that same syringe and it sits for 6 months with those 5 bad mold spores in there. Now those bad ones get to possibly germinate and contam the whole syringe.
Ideally you need to learn to make syringe quickly, its easy, and I am right
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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ShRm22
Shroom Lover


Registered: 03/08/06
Posts: 121
Last seen: 15 years, 5 months
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Re: Spore Print VS Syringe [Re: tahoe]
#7748739 - 12/12/07 11:02 AM (16 years, 1 month ago) |
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Alright I am going to just make prints and save them, only question is how do you seal them? I am going to be using tin foil. Can i rap it with clear rap or will the spores stick to that? When the TEKs say fold the tin foil, do they mean just the edges or fold it in half?
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Spore Print VS Syringe [Re: tahoe]
#7749791 - 12/12/07 03:11 PM (16 years, 1 month ago) |
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Quote:
tahoe said: only make what you will use!!! The print will not contam setting in a sealed bag and there are only so many contams that you can introduce while making a syringe.
Lets say you make a syringe and you get 5 mold spores in there. You use that right away and maybe one injection site goes bad. Pretty good success.
Now you make that same syringe and it sits for 6 months with those 5 bad mold spores in there. Now those bad ones get to possibly germinate and contam the whole syringe.
Ideally you need to learn to make syringe quickly, its easy, and I am right
How will a spore germinate in distilled water? there is nothing for it to feed on.
-------------------- Newcomers guide-----> For all things shroomy
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Funghetto
Little Mushroom



Registered: 11/12/07
Posts: 44
Loc: Europe
Last seen: 15 years, 10 months
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Re: Spore Print VS Syringe [Re: Nibin]
#7749873 - 12/12/07 03:34 PM (16 years, 1 month ago) |
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Must I use distilled water or can I use natural water? Distilled water must be boiled to be sterilized?
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Spore Print VS Syringe [Re: Funghetto]
#7749897 - 12/12/07 03:41 PM (16 years, 1 month ago) |
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Used distilled water like the one they sell for your iron (per il ferro da stiro)
-------------------- Newcomers guide-----> For all things shroomy
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Funghetto
Little Mushroom



Registered: 11/12/07
Posts: 44
Loc: Europe
Last seen: 15 years, 10 months
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Re: Spore Print VS Syringe [Re: Nibin]
#7749919 - 12/12/07 03:50 PM (16 years, 1 month ago) |
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But I have to boiled?
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Spore Print VS Syringe [Re: Funghetto]
#7749932 - 12/12/07 03:53 PM (16 years, 1 month ago) |
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Si
-------------------- Newcomers guide-----> For all things shroomy
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Funghetto
Little Mushroom



Registered: 11/12/07
Posts: 44
Loc: Europe
Last seen: 15 years, 10 months
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Re: Spore Print VS Syringe [Re: Nibin]
#7749955 - 12/12/07 03:59 PM (16 years, 1 month ago) |
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Wow sei italiano!!!  Sai se ci sono altri italiani su questo forum? Grazie dei consigli!!!
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Spore Print VS Syringe [Re: Funghetto]
#7749969 - 12/12/07 04:03 PM (16 years, 1 month ago) |
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Non sono Italiano. Sono Inglese ma abito in Spagna.
Oh and I can hardly write Italian. I can read it and speak it though
-------------------- Newcomers guide-----> For all things shroomy
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Funghetto
Little Mushroom



Registered: 11/12/07
Posts: 44
Loc: Europe
Last seen: 15 years, 10 months
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Re: Spore Print VS Syringe [Re: Nibin]
#7750009 - 12/12/07 04:11 PM (16 years, 1 month ago) |
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Complimenti!!!
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