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Offlineskunkape
earth bound

Registered: 05/29/07
Posts: 1,581
Last seen: 11 years, 10 months
No colonization after 2 weeks
    #7741288 - 12/10/07 03:46 PM (16 years, 1 month ago)

I bought some pre steralized substrate jars online as well as 10ccs of golden teacher spores from the sporestore. Its been two weeks and I cant see any mycelium forming. Well... there are some spots with cloudy white blobs, but im not sure if they were there before or what. Should I keep waiting or should I assume there's something wrong with my substrate or spores?
I've heard both good and bad things about the sporestore, but on my first grow (this is my second time) I ordered the amazon strain and had great results, so I stuck with them again. I believe the golden teachers are supposed to be a quick colonizer... Sorry Im rambling, Im just a little concerned Im going to have to buy more materials and start all over again! :confused:


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Offlineverminute
Stay out of theOTD!
Male

Registered: 11/16/07
Posts: 595
Last seen: 15 years, 10 months
Re: No colonization after 2 weeks [Re: skunkape]
    #7741319 - 12/10/07 03:53 PM (16 years, 1 month ago)

White clouds are first signs of colonization. Make a mark, check back in a few days, has it grown larger? Then just be more patient. Cheers.


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OfflineThe Shape

Registered: 10/27/07
Posts: 328
Loc: Haddonfield, IL Flag
Last seen: 9 years, 9 months
Re: No colonization after 2 weeks [Re: verminute]
    #7741327 - 12/10/07 03:54 PM (16 years, 1 month ago)

ive gone through sporestore 3 times with 3 falures

i believe you may have gotten fake spore as well

i got some ash and water wen i ordered from them


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You miserable, presumptuous, mediocre piece of shit. You have no talent. Your no artist. An artist respects the silence, it serves as the foundation of creativity. Your a disgrace. Your everything thats gone wrong in this world. You're self consumed, no talent, mediocre piece of shit. And i've earned my right to say it.  In 1975 i walked Bob Dylan up on stage. Who the fuck are you?
I knew The Grateful Dead in 1966. Who the fuck are you?
You're nothing... And you'll never be anything.

- Douglas Levison


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Offlineoxohawkoxo
Myconaught
Male

Registered: 10/03/07
Posts: 23
Last seen: 12 years, 29 days
Re: No colonization after 2 weeks [Re: skunkape]
    #7741333 - 12/10/07 03:56 PM (16 years, 1 month ago)

Pics always help to figure out what your 'blobs' are.

There could be a number of factors involved in your lack of growth.

Moisture - If your grain was too wet or has dried out, it will slow any colonization.

Air exchange - No gas exchange = no growth. Not likely a problem as you only need a tiny pathway for grain spawn.

Temperature - As a vaguely general rule, the colder the slower. Too hot will kill them as well.

Shaking your grain immediately after inoculation! - Believe it or not, the mycelia need to 'mate' after hatching from their endospores before they'll reproduce at any significant rate. If you shake the crap out of your grain immediately after inoculation it'll spread them all over and make it more difficult for this to occur in large numbers and they'll get off to a slow start. Sometimes it's a REAL slow start.

If you watch your moisture and wait patiently, they'll show up eventually. By 2 weeks any contams should have showed by now, so I think you're safe. Keep waiting.


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OfflineThe Shape

Registered: 10/27/07
Posts: 328
Loc: Haddonfield, IL Flag
Last seen: 9 years, 9 months
Re: No colonization after 2 weeks [Re: oxohawkoxo]
    #7741365 - 12/10/07 04:03 PM (16 years, 1 month ago)

wen i injected sporestores shit i got no contams the jars just did nothing for months they just sat there looking all saturated and shit


--------------------
You miserable, presumptuous, mediocre piece of shit. You have no talent. Your no artist. An artist respects the silence, it serves as the foundation of creativity. Your a disgrace. Your everything thats gone wrong in this world. You're self consumed, no talent, mediocre piece of shit. And i've earned my right to say it.  In 1975 i walked Bob Dylan up on stage. Who the fuck are you?
I knew The Grateful Dead in 1966. Who the fuck are you?
You're nothing... And you'll never be anything.

- Douglas Levison


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Offlineskunkape
earth bound

Registered: 05/29/07
Posts: 1,581
Last seen: 11 years, 10 months
Re: No colonization after 2 weeks [Re: oxohawkoxo]
    #7741374 - 12/10/07 04:05 PM (16 years, 1 month ago)

Thanks guys. I will shake them now to see if it might help any. I have no real way to regulate the temperature, but I think theyre sitting at around 73 degrees F. I'll stay calm and not make any bad descisions. Peace


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Offlineskunkape
earth bound

Registered: 05/29/07
Posts: 1,581
Last seen: 11 years, 10 months
Re: No colonization after 2 weeks [Re: The Shape]
    #7741381 - 12/10/07 04:07 PM (16 years, 1 month ago)

Quote:

NIBRAL said:
wen i injected sporestores shit i got no contams the jars just did nothing for months they just sat there looking all saturated and shit



yeah thats what Im fearing... I dont want to waste my time with these if they arent going to do anything. If theyre bunk I want to hurry up and get more spores and substrate you know what I mean


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Offlineoxohawkoxo
Myconaught
Male

Registered: 10/03/07
Posts: 23
Last seen: 12 years, 29 days
Re: No colonization after 2 weeks [Re: The Shape]
    #7741389 - 12/10/07 04:09 PM (16 years, 1 month ago)

I've no experience with sporestore myself, but I do know that if you have too much water in your jars there can be bacterial infection. It smells pretty bad, you'd know right away if you opened a jar and took a little whiff.


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OfflineThe Shape

Registered: 10/27/07
Posts: 328
Loc: Haddonfield, IL Flag
Last seen: 9 years, 9 months
Re: No colonization after 2 weeks [Re: skunkape]
    #7741423 - 12/10/07 04:16 PM (16 years, 1 month ago)

Quote:

skunkape said:
Quote:

NIBRAL said:
wen i injected sporestores shit i got no contams the jars just did nothing for months they just sat there looking all saturated and shit



yeah thats what Im fearing... I dont want to waste my time with these if they arent going to do anything. If theyre bunk I want to hurry up and get more spores and substrate you know what I mean




i would ditch half the jars to make room for some SporeWorks

ive personally tested 6 spore vendors and ive had the best succes with sporeworks

oh yea dont mess with pre mixed sloutions & syringes , most everbody will dilute the shit out of it if there even real spores

so go with a print (thats the #1 rule in my mycological book)
there best for microscopical observations  :blowjob:


--------------------
You miserable, presumptuous, mediocre piece of shit. You have no talent. Your no artist. An artist respects the silence, it serves as the foundation of creativity. Your a disgrace. Your everything thats gone wrong in this world. You're self consumed, no talent, mediocre piece of shit. And i've earned my right to say it.  In 1975 i walked Bob Dylan up on stage. Who the fuck are you?
I knew The Grateful Dead in 1966. Who the fuck are you?
You're nothing... And you'll never be anything.

- Douglas Levison


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Offlineskunkape
earth bound

Registered: 05/29/07
Posts: 1,581
Last seen: 11 years, 10 months
Re: No colonization after 2 weeks [Re: oxohawkoxo]
    #7744163 - 12/11/07 08:40 AM (16 years, 1 month ago)

Quote:

oxohawkoxo said:
I've no experience with sporestore myself, but I do know that if you have too much water in your jars there can be bacterial infection. It smells pretty bad, you'd know right away if you opened a jar and took a little whiff.



Hmmm. These jars smell pretty bad like smelly feet. But they've smelled that way ever since I recieved them (before knockin them up. Heres what theyre made up of...
~Organic Brown Rice Flour
~Medium Grained Vermiculite
~Worm Casings
~Filtered Water
~Bee Pollen
Its dark in coloration and has always seemed somewhat damp. Should I complain to the seller or what?


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Offlineenesi
On the Bus
Male User Gallery


Registered: 04/23/06
Posts: 1,408
Loc: Erf
Last seen: 12 years, 5 months
Re: No colonization after 2 weeks [Re: skunkape]
    #7744240 - 12/11/07 09:19 AM (16 years, 1 month ago)

they are are brf jars, you don't shake them at all. that's only for grains.

it just takes time, 2 weeks from multispore isn't very long. Just be patient.


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