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Offlinedeadatdusk
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first lc *DELETED*
    #7739282 - 12/10/07 05:33 AM (16 years, 1 month ago)

Post deleted by deadatdusk

Reason for deletion: deleted



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Offlineozzyozzyozzy
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Re: first lc [Re: deadatdusk]
    #7739302 - 12/10/07 05:42 AM (16 years, 1 month ago)

Ouch. My syringes aren't long enough to meet the water from the holes in the top of my salsa jar lid, so I just crack the top open. Not so great for contams but if you're good with the rest of your sterile techniques, it works alright. I haven't had a problem so far.


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Offlinedeadatdusk
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Re: first lc [Re: ozzyozzyozzy]
    #7739307 - 12/10/07 05:47 AM (16 years, 1 month ago)

What do you use for gas exchange? I innoced immediately after removing foil and spraying 91% alcohol on top of the lid (soaking polyfill), perhaps this will work to my advantage. Though, I'm hoping the fibers I pushed in were from the polyfill on the inside of the jar, in which case it would be sterile. I'm hoping it doesn't hinder growth, or jam my syringe when I go to suck up the culture given that it does successfully colonize.


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Offlineveda_sticks
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Re: first lc [Re: ozzyozzyozzy]
    #7739308 - 12/10/07 05:47 AM (16 years, 1 month ago)

u can get round that by tilting the jar.


--------------------

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Franks Spawning To Bulk - Monotub
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Foo Mans No Soak WBS Prep Tek


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Offlineozzyozzyozzy
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Re: first lc [Re: veda_sticks]
    #7739317 - 12/10/07 05:54 AM (16 years, 1 month ago)

To be honest my LCs are pretty bad. I'm not sure where to get tyvek or polyfill, I just half-fill with honey water and sterilize, cool then innoc. Every day or so I'll swirl it around and hope the water gets aerated. It worked for my first one, hopefully the second one will do alright too. My main effort goes into making sure my spawn bags are okay, so long as there's some myc in the LC that I can innoc with, I'm not fussed.


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Offlinedeadatdusk
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Re: first lc [Re: ozzyozzyozzy]
    #7798457 - 12/25/07 02:22 PM (16 years, 1 month ago)

Just an update on this, my LC colonized nicely and completely with absolutely zero contam rate DESPITE the polyfill fibers floating around. Now, I just need to know where to find a syringe needle long enough to reach the bottom of the jar.


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Offlineozzyozzyozzy
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Re: first lc [Re: deadatdusk]
    #7814759 - 12/30/07 06:35 PM (16 years, 1 month ago)

Stir/shake and then tilt the jar towards the hole in the top :smile:


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OfflineMrAbstraction
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Re: first lc [Re: ozzyozzyozzy]
    #7814777 - 12/30/07 06:39 PM (16 years, 1 month ago)

Also, from what I understand, it's impossible to tell if your LC is contaminated without actually testing it in a sutiable substrate. You might want to think about using a tester jar before knocking up a bunch of projects.

/ma


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InvisibleBlutjager
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Re: first lc [Re: MrAbstraction]
    #7816258 - 12/31/07 08:29 AM (16 years, 1 month ago)

Quote:

MrAbstraction said: You might want to think about using a tester jar before knocking up a bunch of projects.

/ma


:thumbup:Good idea:thumbup:


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Offliner311ik
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Re: first lc [Re: Blutjager]
    #7816435 - 12/31/07 09:35 AM (16 years, 1 month ago)

is there a thread i can read about how myc that isint contamed can become contamed when transfered out of the lc jar?
i thought once the myc started growing it could fight off intruders?


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InvisibleBlutjager
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Re: first lc [Re: r311ik]
    #7817313 - 12/31/07 02:32 PM (16 years, 1 month ago)

Quote:

r311ik said:
i thought once the myc started growing it could fight off intruders?




This is mostly the truth for FULLY colonized substrates,in liquid contamination is harder to spot,if the liquid around the mycelium is cloudy than its more than likely a victim of bacterial contamination but even if its not i could be suspect,its always good to test it out :thumbup:


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Offliner311ik
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Re: first lc [Re: Blutjager]
    #7817621 - 12/31/07 03:58 PM (16 years, 1 month ago)

no cloudiness, clear water with a blob in it. random smell tests never gave me anything unlpesant. usually honey and what i believe to be the smell of myc. the honey smell is really faint now tho.


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OfflineMrAbstraction
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Re: first lc [Re: r311ik]
    #7817737 - 12/31/07 04:35 PM (16 years, 1 month ago)

Here's the word from RR on LC's and contams... I still like LC's even after what he says, but I take the contam portion to heart;

http://www.shroomery.org/forums/showflat.php/Number/7710006#7710006

/ma


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OfflineMayorMcCheese
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Re: first lc [Re: MrAbstraction]
    #7818302 - 12/31/07 08:12 PM (16 years, 1 month ago)

^ I did not know that.


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Offlinemushies101
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Re: first lc [Re: MayorMcCheese]
    #7818465 - 12/31/07 09:30 PM (16 years, 1 month ago)

That sucks. I got a 100ml LC of Hawaiian that I was depending on and now I am scetchy about using it.


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OfflinePsilocin Dreams
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Registered: 08/03/07
Posts: 187
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Re: first lc [Re: mushies101]
    #7818507 - 12/31/07 09:55 PM (16 years, 1 month ago)

Label your LCs and test them out on 1/2 pint BRF cakes. Always test each LC on at least two cakes that way you know it's the LC that is contamed and the contam didn't come from your inoculation procedure. The first time I made LC it looked fine so I knocked up 6 jars, all of them came out contamed. Label the jars, label the two test cakes. If both cakes get contamed, call it a bad LC. If only one goes bad, it's poor sterility in the inoculation procedure. If they're both good, go ahead and turn that LC into a working LC by sucking some up and shooting it into a bigger jar of clean LC material. Good luck, I just recently started working with LC and I don't have enough substrate to even think about using half of it.


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Offlinedeadatdusk
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Re: first lc [Re: Psilocin Dreams]
    #7819161 - 01/01/08 07:21 AM (16 years, 1 month ago)

I PCed some 1/2 pints with a wbs/verm/gypsum mixture i will noc these up and see if they contam from the lc. thanks for the advice guys.


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