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katfish
Nooblit



Registered: 07/22/06
Posts: 592
Loc: Louisiana
Last seen: 15 years, 11 months
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Amazon WBS problems
#7738815 - 12/09/07 11:29 PM (16 years, 1 month ago) |
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I have had this amazon jar, WBS that I bought presterilized and nocced up. Well its the oldest jar I have colonizing in my incubator and it has been producing some metabolites and looking nasty, but I have had this happen with mycobags and eventually the cake sucked back up the metabolites during the last stages of colonization, well thats not happening. Its like the top and inside cylender of the cake is colonized but the rest of the red millet and stuff from the birdseed is all mushy and wet.
heres a pic

My question is can I just open it and scoop just the good colonized material out and spawn to straw?, I have some straw soaking now.
kF
-------------------- 08/2/07 Inoculated mycobag with Brazilian, 3cc 08/08/07 First mycelium show 09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui 10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized 10/12/07 Storage bin perite FC constructed 10/28/07 Havested my First ever flush, of PESA!
Edited by katfish (12/09/07 11:29 PM)
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Amazon WBS problems [Re: katfish]
#7739458 - 12/10/07 07:00 AM (16 years, 1 month ago) |
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I'd try and do a grain to grain transfer in a glovebox first, to make sure there is no contams
-------------------- Newcomers guide-----> For all things shroomy
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katfish
Nooblit



Registered: 07/22/06
Posts: 592
Loc: Louisiana
Last seen: 15 years, 11 months
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Re: Amazon WBS problems [Re: Nibin]
#7739512 - 12/10/07 07:32 AM (16 years, 1 month ago) |
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Ok, but then is it ok to separate the good clean bright white part from the uncolonized part if its not contamed?
That will be a good first task, when I complete my golvebox later today or tomorrow.
kF
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Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
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Re: Amazon WBS problems [Re: katfish]
#7739733 - 12/10/07 09:33 AM (16 years, 1 month ago) |
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problem is, you don't know for sure that all that white mycelium is cube mycelium, which is why I say, use new jars and put little bits from different areas of a healthy looking part of the cake and see what grows. If they all colonize well, it will be a good sign that you have healthy mycelium to work with, instead of risking spawning the hay with contaminated mycelium.
-------------------- Newcomers guide-----> For all things shroomy
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whereismymind
they keepcoming!



Registered: 04/12/04
Posts: 365
Loc: in the middle of nowhere
Last seen: 8 years, 6 months
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Re: Amazon WBS problems [Re: Nibin]
#7739771 - 12/10/07 09:52 AM (16 years, 1 month ago) |
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can't be sure from one picture but that looks nasty .. and if you don't have any other way no other spore syringes prints etc .. i wouldn't just loose time with trying such things
-------------------- In my own summer , there is no crowd in the streets and no sun...
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Cheesekiller
Mad Scientist



Registered: 11/08/04
Posts: 485
Loc: Central NY
Last seen: 1 month, 1 day
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Id say try again. Sterilize yourself, and follow a good tek like this one. I use different lids, home made, but you should be able to get 100% colonized like the pic below in about a week. I incubate at 80F.

Your jar has WAY too much liquid in it.
-CK
-------------------- Bulk grower with "some" success. Cloning Machine Nice Lids A few pics of my DT setup
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Sillicybin
Registered: 02/14/05
Posts: 2,134
Loc:
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Throw that jar out. It is infested with bacterial contamination. I wager if you give that jar a whiff, it smells like crap, which is exactly what all that goo is - the excretions from bacteria munching on the grain.
Either bacteria was introduced during the inoculation process, or the jar wasn't sterilized completely. Increase PC time or check your procedures.
EDIT: Grain that is too WET will often encourage bacterial contamination. If this is the last jar in the batch that you loaded, it could be that this jar's grain was wetter than the others - the grain at the bottom of your container (colander?) had more water in it than the grain at the top.
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desufnoC
Stranger


Registered: 11/04/07
Posts: 114
Last seen: 16 years, 1 month
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Re: Amazon WBS problems [Re: Sillicybin]
#7740469 - 12/10/07 12:50 PM (16 years, 1 month ago) |
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same here
-------------------- fuck captin planet!
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katfish
Nooblit



Registered: 07/22/06
Posts: 592
Loc: Louisiana
Last seen: 15 years, 11 months
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Re: Amazon WBS problems [Re: desufnoC]
#7742246 - 12/10/07 07:07 PM (16 years, 1 month ago) |
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ok, i though it was metabolites like i have had in mycobags, i have smelt it and it smells like wbs. so I didn't think contamed.
CONTAMINATED!!!!
this thead is done!
kF
-------------------- 08/2/07 Inoculated mycobag with Brazilian, 3cc 08/08/07 First mycelium show 09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui 10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized 10/12/07 Storage bin perite FC constructed 10/28/07 Havested my First ever flush, of PESA!
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