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Offlinekatfish
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Registered: 07/22/06
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Loc: Louisiana
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Amazon WBS problems
    #7738815 - 12/09/07 11:29 PM (16 years, 1 month ago)

I have had this amazon jar, WBS that I bought presterilized and nocced up. Well its the oldest jar I have colonizing in my incubator and it has been producing some metabolites and looking nasty, but I have had this happen with mycobags and eventually the cake sucked back up the metabolites during the last stages of colonization, well thats not happening. Its like the top and inside cylender of the cake is colonized but the rest of the red millet and stuff from the birdseed is all mushy and wet.

heres a pic




My question is can I just open it and scoop just the good colonized material out and spawn to straw?, I have some straw soaking now.

kF


--------------------
08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!


Edited by katfish (12/09/07 11:29 PM)


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OfflineNibin
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Re: Amazon WBS problems [Re: katfish]
    #7739458 - 12/10/07 07:00 AM (16 years, 1 month ago)

I'd try and do a grain to grain transfer in a glovebox first, to make sure there is no contams


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Offlinekatfish
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Re: Amazon WBS problems [Re: Nibin]
    #7739512 - 12/10/07 07:32 AM (16 years, 1 month ago)

Ok, but then is it ok to separate the good clean bright white part from the uncolonized part if its not contamed?

That will be a good first task, when I complete my golvebox later today or tomorrow.

kF


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OfflineNibin
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Re: Amazon WBS problems [Re: katfish]
    #7739733 - 12/10/07 09:33 AM (16 years, 1 month ago)

problem is, you don't know for sure that all that white mycelium is cube mycelium, which is why I say, use new jars and put little bits from different areas of a healthy looking part of the cake and see what grows. If they all colonize well, it will be a good sign that you have healthy mycelium to work with, instead of risking spawning the hay with contaminated mycelium.


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Newcomers guide-----> For all things shroomy


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Offlinewhereismymind
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Re: Amazon WBS problems [Re: Nibin]
    #7739771 - 12/10/07 09:52 AM (16 years, 1 month ago)

can't be sure from one picture but that looks nasty .. and if you don't have any other way no other spore syringes prints etc .. i wouldn't just loose time with trying such things


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In my own summer , there is no crowd in the streets and no sun...


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OfflineCheesekiller
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Registered: 11/08/04
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Re: Amazon WBS problems [Re: whereismymind]
    #7740080 - 12/10/07 11:26 AM (16 years, 1 month ago)

Id say try again. Sterilize yourself, and follow a good tek like this one. I use different lids, home made, but you should be able to get 100% colonized like the pic below in about a week. I incubate at 80F.



Your jar has WAY too much liquid in it.

-CK


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Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup


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InvisibleSillicybin
Registered: 02/14/05
Posts: 2,134
Loc: Flag
Re: Amazon WBS problems [Re: Cheesekiller]
    #7740212 - 12/10/07 11:55 AM (16 years, 1 month ago)

Throw that jar out. It is infested with bacterial contamination. I wager if you give that jar a whiff, it smells like crap, which is exactly what all that goo is - the excretions from bacteria munching on the grain.

Either bacteria was introduced during the inoculation process, or the jar wasn't sterilized completely. Increase PC time or check your procedures.

EDIT: Grain that is too WET will often encourage bacterial contamination. If this is the last jar in the batch that you loaded, it could be that this jar's grain was wetter than the others - the grain at the bottom of your container (colander?) had more water in it than the grain at the top.


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OfflinedesufnoC
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Registered: 11/04/07
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Re: Amazon WBS problems [Re: Sillicybin]
    #7740469 - 12/10/07 12:50 PM (16 years, 1 month ago)

same here


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fuck captin planet!


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Offlinekatfish
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Re: Amazon WBS problems [Re: desufnoC]
    #7742246 - 12/10/07 07:07 PM (16 years, 1 month ago)

ok, i though it was metabolites like i have had in mycobags, i have smelt it and it smells like wbs. so I didn't think contamed.


CONTAMINATED!!!!

this thead is done!

kF


--------------------
08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!


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