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OfflineLordByron
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Registered: 10/07/05
Posts: 33
Loc: Cascadia
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Several Psilocybe cyanescens questions
    #7729585 - 12/07/07 04:41 PM (16 years, 1 month ago)

Hey all,
First off please excuse me if these are not exactly super "advanced mycology" questions. As a long time lurker on this board, I was afraid questions about anything other than cubes on the basic forum would not be helpful. On to the point...

So I live in Washington and want my own outdoor Psilocybe cyanescens patch. I have found these mushrooms several times in wild but never in enough quantity or quality to suffice. So recently I acquired an autoclave, a culture incubator, and built a laminar flow hood (thanks in no small part to this thread). I then hunted down a few wild specimens and have cloned them onto several petri dishes of MEA. Why MEA? Because that’s what I ordered before I read the part of “growing gourmet…” where Stamets recommends MYA, PDYA, OMYA, or DFA. But this is still a little confusing to me because two pages earlier (P. 326 in my edition) he shows a picture of P. azurescens growing on MEA. So first question, did I fuck up? Should I have used DFA or something?

Regardless, I now have several dishes with a very smooth velvety mycelium growing on them. Now on to my second question, should I be looking for rhizomorphic growth? Is the lack of rhizomorphic growth due to the MEA or the fact that I have not sectored the culture enough times yet?

My plan now was to do one more round of sectoring either on to MEA again or DFA depend on what you guys say. From there I wanted to make up a bunch of half gallon jar masters, but I am not sure what medium would be best. I am considering rye grain, wood chips (maple), and card board. The final fruiting substrate will be likely a mostly maple wood chip matrix. So should I do a couple rye grain masters then use these to inoculate woodchip masters? Or should I go first to card board then to wood chip masters? Or straight to wood chip masters? Or some combination not listed?

Lastly, I decided to hedge my bets and very recently ordered several prints so if a terminally screw up one I will have other strains to go to. I ordered two other cyanescens strains as well as Psilocybe arcana (Hlavacek’s Psilocybe) and a wild strain of Psilocybe cyanofibrillosa. The arcana is supposedly a temperate mushroom from the Czech republic that I really have no idea whether I will be able to get it to grow outdoors in Washington, but am going to give it a go. The cyanofibrillosa is a species I have only ever found in terrible states of decay in the wild and I would really like to try. While I’m sure it will grow outdoors in Washington I’m not even sure it will even get me f’d up. Does anyone have any experience growing either of these two species?

Thank you very much for any assistance now and in the past.


--------------------
All you touch and all you see, is all your life you'll ever be.


Edited by LordByron (12/07/07 04:43 PM)


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OfflineLordByron
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Registered: 10/07/05
Posts: 33
Loc: Cascadia
Last seen: 5 years, 1 month
Re: Several Psilocybe cyanescens questions [Re: tahoe]
    #7765745 - 12/16/07 02:14 PM (16 years, 1 month ago)

I think things are coming along well... This what it should look like?


Only a little bit of contamination....


--------------------
All you touch and all you see, is all your life you'll ever be.


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OfflineLordByron
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Registered: 10/07/05
Posts: 33
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Re: Several Psilocybe cyanescens questions [Re: tahoe]
    #7773274 - 12/18/07 10:11 AM (16 years, 1 month ago)

I'm not exactly sure if they are from spores or transfers. By the time I got my lab set up I could only find two very small buttons before we got a hard frost and pretty much ended hunting for the year. I dipped the specimens in a 90% methanol solution and then rinsed them with distilled water. The buttons were very small when I cut them and they gills seems barely mature if at all. I know normally you want to take a cutting from the center part of the stalk or from just under the cuticle of the cap, but I had a very small amount of raw material to work with so I just cleaned them as described above and then cut them (gills and all) into small sections.


--------------------
All you touch and all you see, is all your life you'll ever be.


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OfflineLordByron
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Re: Several Psilocybe cyanescens questions [Re: RogerRabbit]
    #7830133 - 01/03/08 10:53 PM (16 years, 28 days ago)

As it happens right now I am attempting to grow spawn on both cardboard and rye grain.


rye grain


cardboard

The mycelium growing on the card board is ropey and beautiful but there is also much less of it.


--------------------
All you touch and all you see, is all your life you'll ever be.


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OfflineLordByron
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Registered: 10/07/05
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Last seen: 5 years, 1 month
Re: Several Psilocybe cyanescens questions [Re: Topcorn]
    #7833685 - 01/04/08 09:28 PM (16 years, 27 days ago)

As of right now the plan is to put the spawn into spawn bags with sterilized wood chips. These bags will be used this spring to establish an outdoor bed. I’m not sure if that is how I will use 100% of the rye spawn but the bulk will be used like that. With the difference, I was toying with the idea of layered wood spawn generation. I recently read a thread on another site that showed some incredible results with other wood lovers by slowly adding layers of wood chips to a non-sterile bed. I don’t know if I can produce the right conditions for this to work but it seems like a fun experiment.


--------------------
All you touch and all you see, is all your life you'll ever be.


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