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armyabe
Mimic



Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
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what does contamed lc look like?
#7728041 - 12/07/07 10:04 AM (16 years, 1 month ago) |
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I have three lc they appear fine however i'm not sure what to look for
-------------------- What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City
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armyabe
Mimic



Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
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Re: what does contamed lc look like? [Re: armyabe]
#7728052 - 12/07/07 10:06 AM (16 years, 1 month ago) |
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oh, and how long should i stir them for minute, all the time?
-------------------- What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: what does contamed lc look like? [Re: armyabe]
#7728092 - 12/07/07 10:15 AM (16 years, 1 month ago) |
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Generaly its very hard to tell if an lc is contammed, The onlys ure fire way of telling is to test it on 1 jar of substrate first.
If the water is cloudy, that is an indication of contamination. But apart from that theres nothing else, moulds wont sporolate in LC so its possibl to have mould mycelium and not know.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: what does contamed lc look like? [Re: veda_sticks]
#7728131 - 12/07/07 10:26 AM (16 years, 1 month ago) |
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Quote:
What does contamed lc look like?
Usually, exactly like non-contaminated lc. That's why I've never recommended them. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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armyabe
Mimic



Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
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Re: what does contamed lc look like? [Re: RogerRabbit]
#7728336 - 12/07/07 11:18 AM (16 years, 1 month ago) |
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oh you don't suggest LC?
-------------------- What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: what does contamed lc look like? [Re: armyabe]
#7728381 - 12/07/07 11:33 AM (16 years, 1 month ago) |
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cloudy, milky, bubblely, thin white residue on the sides of the jar after swirreling
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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MotorCityMadman
Rubberfan andFunketeer



Registered: 10/02/06
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Loc: Motown (State of Mind)
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Re: what does contamed lc look like? [Re: RogerRabbit]
#7728416 - 12/07/07 11:44 AM (16 years, 1 month ago) |
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Quote:
RogerRabbit said:
Quote:
What does contamed lc look like?
Usually, exactly like non-contaminated lc. That's why I've never recommended them. RR
ya beat me to it, RR!
agar & g2g rules, imho.
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armyabe
Mimic



Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
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so when use do grain to grain do you introduce h2o2?
-------------------- What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: what does contamed lc look like? [Re: armyabe]
#7728789 - 12/07/07 01:38 PM (16 years, 1 month ago) |
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No. Peroxide is no substitute for sterile procedure. Peroxide will wipe out cobweb mold on a casing layer, but it has little other use, imho of course. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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armyabe
Mimic



Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
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Re: what does contamed lc look like? [Re: RogerRabbit]
#7728874 - 12/07/07 02:00 PM (16 years, 1 month ago) |
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i thought it was used for prevention also. much to learn
-------------------- What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Re: what does contamed lc look like? [Re: armyabe]
#7728920 - 12/07/07 02:11 PM (16 years, 1 month ago) |
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no, its a commen misconception, when i started i was trying to find where to get h202, well i have never needed to use it.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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armyabe
Mimic



Registered: 09/13/07
Posts: 240
Loc: Washington state
Last seen: 16 years, 23 days
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Re: what does contamed lc look like? [Re: veda_sticks]
#7728955 - 12/07/07 02:19 PM (16 years, 1 month ago) |
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i have read alot of studies on it and i heard it was a huge breakthrough when it was discovered that mycelem was h2o2 tolerant. weird ok i'll forget about it.
-------------------- What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City
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Fraggin
Multi-Faceted



Registered: 01/05/05
Posts: 8,707
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Re: what does contamed lc look like? [Re: armyabe]
#7729113 - 12/07/07 02:56 PM (16 years, 1 month ago) |
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You can use h202 to clean your work surfaces before you do mycological work. And maybe add a bit to the water in your atomizer you use to spray your terrarium.... If your aquarium pump breaks, you can add some to the fish's water to give them enough oxygen to live until you can make it to the pet store.
Otherwise, it's just a mild oxidizing agent.
Hydrogen peroxide is another chemical sterilizing agent. It is relatively non-toxic once diluted to low concentrations (although a dangerous oxidizer at high concentrations), and leaves no residue.
Sterrad sterilization chambers use hydrogen peroxide vapor to sterilize heat-sensitive equipment such as rigid endoscopes. A recent model can sterilize most hospital loads in as little as 20 minutes. The Sterrad has limitations with processing certain materials such as paper/linens and long thin lumens. Paper products cannot be sterilized in the Sterrad system because of a process called cellulostics, in which the hydrogen peroxide would be completely absorbed by the paper product.
Hydrogen peroxide and formic acid are mixed as needed in the Endoclens device for sterilization of endoscopes. This device has two independent asynchronous bays, and cleans (in warm detergent with pulsed air), sterilizes and dries endoscopes automatically in 30 minutes. Studies with synthetic soil with bacterial spores showed the effectiveness of this device.
Dry sterilization process (DSP) uses hydrogen peroxide at a concentration of 30-35% under low pressure conditions. This process achieves bacterial reduction of 10-6...10-8. The complete process cycle time is just 6 seconds, and the surface temperature is increased only 10-15 °C (18 to 27 °F). Originally designed for the sterilization of plastic bottles in the beverage industry, because of the high germ reduction and the slight temperature increase the dry sterilization process is also useful for medical and pharmaceutical applications.
Peracetic acid (0.2%) is used to sterilize instruments in the Steris system.
Prions are highly resistant to chemical sterilization. Treatment with aldehydes (e.g., formaldehyde) have actually been shown to increase prion resistance. Hydrogen peroxide (3%) for one hour was shown to be ineffective, providing less than 3 logs (10-3) reduction in contamination. Iodine, formaldehyde, glutaraldehyde and peracetic acid also fail this test (one hour treatment). Only chlorine, a phenolic compound, guanidinium thiocyanate, and sodium hydroxide (NaOH) reduce prion levels by more than 4 logs. Chlorine and NaOH are the most consistent agents for prions. Chlorine is too corrosive to use on certain objects. Sodium hydroxide has had many studies showing its effectiveness.
Edited by Fraggin (12/07/07 03:01 PM)
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Fraggin
Multi-Faceted



Registered: 01/05/05
Posts: 8,707
Last seen: 8 years, 3 days
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Re: what does contamed lc look like? [Re: Fraggin]
#7729119 - 12/07/07 02:57 PM (16 years, 1 month ago) |
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BTW, Contaminated LC can look just like a healthy lc. There is no way to tell except innoc some substrate.
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