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OfflineCallampin
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Iodine vs H2O2
    #7727805 - 12/07/07 09:01 AM (16 years, 1 month ago)

very recently i ventured into cloning, not much luck yet due to contams and bad conditions , ive dipped / sprayed etc h2o2 solution in everythign , and it still contams

so lately ive read about using iodine  2cc per liter , asuming  im going to use the tipical iodine tincture  used for cuts etc .

does anyone have experience using this >? from what ive read it will kill most contams :supercrankey:


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Offlineveda_sticks
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Re: Iodine vs H2O2 [Re: Callampin]
    #7727815 - 12/07/07 09:05 AM (16 years, 1 month ago)

Not sure about iodine.

h202 isnt that effective and will most likely hurt the the tissue more than the contam.

I have heard that a bleach solution can be used to soak the tissue for a few minutes to clean it up.

Theres always the option of using agar and growing it out that way. Then if a contams shows up, tranfer a clean peice of tissue to another plate. Continue transfering until the contam is gone.

Have a look at the agar teks.

Also, are you taking a sample from the very middle?? Which in thoery should b the cleanest part.


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OfflineCallampin
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Re: Iodine vs H2O2 [Re: veda_sticks]
    #7728148 - 12/07/07 10:31 AM (16 years, 1 month ago)

yeah , im using the center part, ive never used the outer skin for cloning , im getting malt agar soon tho


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