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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: vacum pulled spores into lc... [Re: RogerRabbit]
    #7724971 - 12/06/07 03:23 PM (16 years, 1 month ago)

RR- what do you think of my suggestion of piercing a hole in the lids of my 3 lc jars in a glove box for a ge port? or will they be okay with out one. there is 400 ml of medium in a quart.

under sterile conditions of course..

would it suck in a bunch of air being that its probabally under a vacum?


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OfflineRogerRabbitM
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Re: vacum pulled spores into lc... [Re: blood4blood]
    #7725119 - 12/06/07 03:57 PM (16 years, 1 month ago)

Wrap an alcohol soaked paper towel around the nail when you pierce the lid. The towel will act as a filter.
RR


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: vacum pulled spores into lc... [Re: RogerRabbit]
    #7729112 - 12/07/07 02:55 PM (16 years, 1 month ago)

so i just decided to bump this again instead of starting a new thread.


so i went ahead and took RR's advice and used a alcohol soaked paper towel and piereced my lc jars. (good thing i did this because i heard air whoosing in as soon as i pierced the lid)

my question is- when i piereced the lid with the nail i noticed that a few drops of alcohol dripped down the nail and into the lc medium. will this be a problem? will it kill the spores?


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OfflineFraggin
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Re: vacum pulled spores into lc... [Re: blood4blood]
    #7729124 - 12/07/07 02:57 PM (16 years, 1 month ago)

A few drops? I say no.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: vacum pulled spores into lc... [Re: Fraggin]
    #7729278 - 12/07/07 03:34 PM (16 years, 1 month ago)

I agree, that shouldn't be too much trouble.

be careful to never get the micropore tape you put over those
holes wet.
if you do, expect certain contamination.

wet micropore tape serves as a wick to introduce contams into
the jar, which is no good.

getting it wet with the sugar-laden LC liquid is in even worse idea.

so just be careful and keep that micropore dry.


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OfflineBlargIAmDead
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Registered: 11/04/07
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Re: vacum pulled spores into lc... [Re: monstermitch]
    #7729363 - 12/07/07 03:59 PM (16 years, 1 month ago)

Okay, I have a question. I tape my jars before I foil wrap and boil them. It is inevitable the tape gets wet. What would be the proper procedure for sterility's sake? Should I leave them in the pot, with the foil covered till I'm ready to inoculate, or can I take them out of the pot, remove the foil, and put them (still pretty warm) into a still air box to cool off since there's a dry verm barrier? Or should I just leave the tape off period till after I inoculate?


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: vacum pulled spores into lc... [Re: BlargIAmDead]
    #7729579 - 12/07/07 04:39 PM (16 years, 1 month ago)

if there's a dry verm layer then there is no need for the tape.


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