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x7x_x7x
x7x, my problem child.




Registered: 05/05/07
Posts: 3,816
Loc: buenos aires
Last seen: 20 hours, 33 minutes
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growing seeds on agar?
#7719447 - 12/05/07 12:13 PM (16 years, 1 month ago) |
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yesterday i saw a TV documentary on blue berrys industry. they grow BB in agar jars or something that seems it. anybody get data on grow plants in agar?
-------------------- cultivando en la miseria SuctoSpore® Pictorial Tek
 x7x_x7x@shroomery.org carl_jung_in_lsd@yahoo.com koh samui and oak ridge are my favourite strains
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royer
±±±±±±±±±±


Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
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Re: growing seeds on agar? [Re: x7x_x7x]
#7719462 - 12/05/07 12:20 PM (16 years, 1 month ago) |
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lots of different plants are started on agar through tissue culturing, there are also lots of different kinds of agar and additives
i use to buy orchids by the flask which was 50-100 plants on agar from tissue culturing
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
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YidakiMan
Stranger


Registered: 09/28/02
Posts: 2,023
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Re: growing seeds on agar? [Re: royer]
#7719487 - 12/05/07 12:26 PM (16 years, 1 month ago) |
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Hosta industry also relies on tissue culture.
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x7x_x7x
x7x, my problem child.




Registered: 05/05/07
Posts: 3,816
Loc: buenos aires
Last seen: 20 hours, 33 minutes
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Re: growing seeds on agar? [Re: royer]
#7719703 - 12/05/07 01:41 PM (16 years, 1 month ago) |
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amazing!
two question raise: agar composition and how to avoid contams, a seed is covered for hundreds of microorganisms. gentamicin?
-------------------- cultivando en la miseria SuctoSpore® Pictorial Tek
 x7x_x7x@shroomery.org carl_jung_in_lsd@yahoo.com koh samui and oak ridge are my favourite strains
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caricapapaya
Stranger



Registered: 04/10/06
Posts: 258
Last seen: 1 year, 8 months
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Re: growing seeds on agar? [Re: x7x_x7x]
#7719746 - 12/05/07 01:50 PM (16 years, 1 month ago) |
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you can surface sterilize seeds in bleach, and wash in etoh, and rinse in sterile water.
Then place them in culkture.
If the seeds of interest are still in the fruit (pod or whatever), its even easier. You can sterilze with high concentration of bleach and then cut open the fruit in sterile conditions and pull out seeds.
Seeds in an unopened pod should be sterile.
good luck carica
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jben
Don't panic, it's organic


Registered: 05/24/04
Posts: 931
Last seen: 8 years, 4 months
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kinda funny you mention... Although ferns don't have seeds, they have spores. My botany class lab is doing a project with culturing ferns on agar. The supplies and everything are in a package called "C Fern" and it's produced by some scientific product company. we're doing studies on genetics, and some other random stuff.
-------------------- Don't panic, it's organic!
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Scopolia
Stranger

Registered: 10/07/05
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Re: growing seeds on agar? [Re: jben]
#7740476 - 12/10/07 12:52 PM (16 years, 1 month ago) |
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1% agar agar with water works well for germinating many seeds. In addition to the seed sterilization techniques mentioned above, just be prepared to prick out germinating seeds into a proper medium in a timely manner.
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x7x_x7x
x7x, my problem child.




Registered: 05/05/07
Posts: 3,816
Loc: buenos aires
Last seen: 20 hours, 33 minutes
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Re: growing seeds on agar? [Re: Scopolia]
#7782593 - 12/20/07 05:54 PM (16 years, 1 month ago) |
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1% is sufficient for solidifying?
-------------------- cultivando en la miseria SuctoSpore® Pictorial Tek
 x7x_x7x@shroomery.org carl_jung_in_lsd@yahoo.com koh samui and oak ridge are my favourite strains
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undergrounder
fluffy bunny



Registered: 11/10/06
Posts: 1,394
Loc: Sydney
Last seen: 1 year, 7 months
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Re: growing seeds on agar? [Re: x7x_x7x]
#7788096 - 12/22/07 05:31 AM (16 years, 1 month ago) |
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RIP Bigger and bolder and rougher and tougher in other words sucka there is no other...
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x7x_x7x
x7x, my problem child.




Registered: 05/05/07
Posts: 3,816
Loc: buenos aires
Last seen: 20 hours, 33 minutes
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thanks for the data
-------------------- cultivando en la miseria SuctoSpore® Pictorial Tek
 x7x_x7x@shroomery.org carl_jung_in_lsd@yahoo.com koh samui and oak ridge are my favourite strains
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