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Offlinearmyabe
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Registered: 09/13/07
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h2o2 grain spawn
    #7714355 - 12/04/07 08:47 AM (16 years, 1 month ago)

how much h2o2 is to be added to a grain spawn that has been PC for grain to grain inoc, and can it be added to a spore to grain once it has been established?


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What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City


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InvisibleTomandjerry58
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Registered: 01/27/03
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Re: h2o2 grain spawn [Re: armyabe]
    #7714364 - 12/04/07 08:50 AM (16 years, 1 month ago)

no need to "add" just dip ur spoon in it while ur tranfering every 2 scoops. understand?


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Offlinearmyabe
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Re: h2o2 grain spawn [Re: Tomandjerry58]
    #7714372 - 12/04/07 08:53 AM (16 years, 1 month ago)

no that didn't make sense.


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What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City


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InvisibleDJYoshaBYD
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Registered: 04/07/07
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Re: h2o2 grain spawn [Re: armyabe]
    #7714393 - 12/04/07 08:59 AM (16 years, 1 month ago)

no.. do not add h2o2 to your substrate... whatever you are using to transfer the grain (i.e. inoc loop, tweezers, fork, etc.) dip it in the h2o2 after each transfer so that it is sterile..


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Offlinearmyabe
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Re: h2o2 grain spawn [Re: DJYoshaBYD]
    #7714464 - 12/04/07 09:24 AM (16 years, 1 month ago)

a few of the books i have speak of it thats why i asked. i appriciate it.


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What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City


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OfflineRogerRabbitM
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Re: h2o2 grain spawn [Re: armyabe]
    #7714484 - 12/04/07 09:31 AM (16 years, 1 month ago)

Don't use peroxide on grains. Also, don't use tools to make the transfer. Peroxide will not sterilize spoons, forks, etc. Bang the unopened jar against a tire to break up the grains, and then pour the grains from the master jar to the receiving jars without touching them with anything.
RR


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Offlinearmyabe
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Re: h2o2 grain spawn [Re: RogerRabbit]
    #7714688 - 12/04/07 10:29 AM (16 years, 1 month ago)

what if i am transfering from brf? thanks RR


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What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City


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InvisibleSlimz
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Re: h2o2 grain spawn [Re: armyabe]
    #7714711 - 12/04/07 10:33 AM (16 years, 1 month ago)

stick the cake in a bag, crumble it thru the bag, and transfer from the bag(master) to the receiver without touching..


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Offlinearmyabe
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Re: h2o2 grain spawn [Re: Slimz]
    #7714996 - 12/04/07 11:43 AM (16 years, 1 month ago)

gotcha


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What if I am wrong, Ive got a condition, I get confused sometimes, what if I have imaged all of this, what if have finally turned into what they've always said I was gonna turn into.... a maniac...... a psycho killer.....Marv Sin City


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