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juende
feministpresence


Registered: 02/20/04
Posts: 729
Last seen: 8 years, 11 months
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dunking
#7709274 - 12/03/07 12:19 AM (16 years, 1 month ago) |
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is it bad to dunk for too long? lets say one had a few pint jars, maybe PRs and Eq, brf/verm mix, they have been in jars with water for at least 3 days and in some what cooler temps. should anything be done if this is a problem? cakes? casings? danke!
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Azurescendence
Seeker of Knowledge



Registered: 10/21/07
Posts: 72
Loc: NE United States
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Re: dunking [Re: juende]
#7709299 - 12/03/07 12:25 AM (16 years, 1 month ago) |
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Eventually, from what I understand, the mycelium will drown. As far as I know it is unhealthy to dunk for longer than 24 hours, maybe that maximum could be extended a bit if kept at cooler temps... but 72 hours underwater doesn't sound good... I'm not an expert however, it's possible that mycelium can live for longer periods underwater than that, but I would say that regardless of what you decide to use your jars for (cakes/casings/spawn), you should probably get that water out of there asap. (or get them out of the water, whichever way you dunked them) I hope some people with experience dunking for longer than 24 hours can post and shed some experiential light on this... Good luck!
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Nibin
Getting there



Registered: 11/29/05
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The mycelium will die, rot and contam.
Get them out of the water and into a FC and if you are lucky, enough air will have got to them that they haven't died.
The colder temperatures might have helped. Just make sure they don't start to rot or the rest of your cakes might be affected.
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juende
feministpresence


Registered: 02/20/04
Posts: 729
Last seen: 8 years, 11 months
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aye! thats what working two jobs could do to a persons growth. i guess a grow log will have to be started to see if this will work out now, this is turning out to be such a long long and slow ass grow.
another question... has anyone had any probs/contams after dunking?
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juende
feministpresence


Registered: 02/20/04
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Re: dunking [Re: Nibin]
#7709382 - 12/03/07 12:46 AM (16 years, 1 month ago) |
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Quote:
Nibin said: The mycelium will die, rot and contam.
Get them out of the water and into a FC and if you are lucky, enough air will have got to them that they haven't died.
The colder temperatures might have helped. Just make sure they don't start to rot or the rest of your cakes might be affected.
whoops i type too slow, that answered my question
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Nibin
Getting there



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Re: dunking [Re: juende]
#7709383 - 12/03/07 12:46 AM (16 years, 1 month ago) |
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if done in the fridge or under running water the risk of bacterial bloom is very low, and even done somewhere else, the cakes are pretty contam resistant.
But I don't know about a cake weakened by 72 hr dunking...
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JewelessCaesar
Shroomery's Legal Department




Registered: 05/26/06
Posts: 599
Loc: Petros-Joyner, TN, USA
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Re: dunking [Re: juende]
#7709544 - 12/03/07 02:39 AM (16 years, 1 month ago) |
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Was the mycelium dunked in the jar as it was grown in?
Was the mycelium fully submerged? If not I say it lives.
36 hours. Wow. But I understand with all the time away.
For people like you a fully automated Martha might be right up your ally.
Good luck, I hope it survives so you can enjoy the primordia and pinning to come! I'm sure that sometimes it deals with heavy water accumulation in nature in somehow and someway, possibly due to location and precipitation level where ever it is native too. So be optimistic, you never know! 
JCLE
-------------------- Failure is not an option, it's an adventure! - JewelessCaesar
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Nibin
Getting there



Registered: 11/29/05
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Quote:
JewelessCaesar said:
Was the mycelium fully submerged? If not I say it lives.
JCLE
My money is on that one too. If it was floating about and moving, it will probably have got enough air. If it was fully submerged, i'd be a lot more pessimistic.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
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Re: dunking [Re: Nibin]
#7710038 - 12/03/07 09:35 AM (16 years, 1 month ago) |
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I've seen pf type cakes survive two week dunks in the refrigerator. I've also seen master slants under water survive two or three years in the refrigerator.
You shouldn't dunk in the jars. It takes pressure to hydrate the mycelium. Dunking isn't hydrating the substrate, which is fully colonized. Dunking is to hydrate the individual mycelium cells, which takes time and a higher pressure outside the cell wall than inside(osmosis) for hydration to occur. Dunk in a large kettle or bucket, etc., so the cake can be pushed completely under water, thus providing the pressure required. Soak the cakes in this manner for 24 hours. RR
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Fraggin
Multi-Faceted



Registered: 01/05/05
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Quote:
RogerRabbit said:
You shouldn't dunk in the jars. It takes pressure to hydrate the mycelium. Dunking isn't hydrating the substrate, which is fully colonized. Dunking is to hydrate the individual mycelium cells, which takes time and a higher pressure outside the cell wall than inside(osmosis) for hydration to occur. Dunk in a large kettle or bucket, etc., so the cake can be pushed completely under water, thus providing the pressure required. Soak the cakes in this manner for 24 hours. RR
Have you experimented with the solution that you dunk cakes into?
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c0_hush
Stranger



Registered: 07/16/06
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RR, would you still dunk + roll a pf cake if the myc is thin? Would a thin myc suggest no inner colonization? Should keeping these cakes incubated longer promote thicker myc + a true full colonization?
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Manna Inspired
NOT-SO-NOOB



Registered: 11/28/07
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Re: dunking [Re: c0_hush]
#7710075 - 12/03/07 09:47 AM (16 years, 1 month ago) |
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I always submerge for 48+ w/ great results every time.
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juende
feministpresence


Registered: 02/20/04
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well i noticed that the jars that had 50% or less water in it did fine. the others that were fully submerged had this weird clear slime near the bottom of the cake i guess its a go ahead and see.
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Nibin
Getting there



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Re: dunking [Re: juende]
#7714330 - 12/04/07 08:36 AM (16 years, 1 month ago) |
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wash it off under a running tap.
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