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Invisiblelegallyhomeless
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Purple Contam.
    #7701778 - 12/01/07 03:47 AM (16 years, 3 months ago)



Quote:

Ryche Hawk said:
Heya Folks,

A little something my partner -Clyde- learned while spending some time with P. Stamets a while ago is there is a dealy form of purple contamination to watch out for. Their are 2 kinds we can see in in our hobby. One is an obviously harmless form of trichoderma since so many of us have seen it and been exposed to it without any danger. But their is another form wich can be deadly. -Clyde- seen a thread on this and asked me to post this.

The purple contam that shows up late in the colonization of grain spawn can be, and most likely is, Fusarium
sporothichiodes, the cause of spinal menningitis, which has a 98% fatality rate. Do not handle it! It can enter through
small cuts and breaks in the skin. It often heats up the jar/bag. On agar, seemingly fast growing mycelium looks like
blunt ended spikes, short but definately abnormal, but white. This nasty occurs naturaly on grain. It's presence in culture
indicates incomplete sterilization of the grain. Don't frick with it, reseal it in multiple layers, and pitch it. It is very possible
to accidently culture some serious nasties, so exercise caution and good sence. The general rule is to treat all brightly
colored contams, especially purple, pink, orange, and yellow as dangerous. Don't confuse yellowish mycelial
exudate/metabolite with nasties. It's normal and common to see them. Keep yourselves and your buddies safe, and
immediatly dispose of any questionable cultures, jars, lids and all.

I cant tell you how many times in the past I use to clean out contaminated jars not realizing I was playing with molds that were potentially deadly. These days we just pitch the jars etc... instead of worrying about recycling. Food for thought, be safe :smile:

-Peace-
www.thehawkseye.com
Sacred Mushroom Spores



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OfflineRogerRabbitM
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Re: Purple Contam. [Re: legallyhomeless]
    #7702077 - 12/01/07 08:43 AM (16 years, 3 months ago)

You can try to get a closer, clear picture, but your jar doesn't look like fusarium, or any mold for that matter. It's below the metabolite line in the jar. I'd still toss it though of course, since it isn't right. If you need to save the jar itself, PC before opening.
RR


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semper in excretia sumus solim profundum variat

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Invisiblelegallyhomeless
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Re: Purple Contam. [Re: RogerRabbit]
    #7703047 - 12/01/07 02:52 PM (16 years, 3 months ago)

Heh finally RR post on one of my threads :smile:. Not fusarium ay? Hmm, I tried to upload the full size image but I couldnt. I keep all my jars. Usually I just dump my contamed jars into the yard and wash them out with soap and water. Ill get a better picture later.


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Offlinemilkman
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Re: Purple Contam. [Re: legallyhomeless]
    #7703461 - 12/01/07 04:46 PM (16 years, 3 months ago)

emo jars killing them self with they're own metabolites jk

i dunno what its called but if it is a mold or a unwanted growth it could be very bad for your health

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Invisiblelegallyhomeless
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Re: Purple Contam. [Re: milkman]
    #7704670 - 12/01/07 10:40 PM (16 years, 3 months ago)

Maybe this one will be better?


I need a new camera, mine has been dropped so many times it focuses crazy.


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OfflineRogerRabbitM
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Re: Purple Contam. [Re: legallyhomeless]
    #7705845 - 12/02/07 08:41 AM (16 years, 3 months ago)

Bacteria. Molds don't sporulate in liquid. I can see the signs of bacterial infection above the metabolite line as well. PC before opening, or toss in the garbage.
RR


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Re: Purple Contam. [Re: RogerRabbit]
    #7706999 - 12/02/07 03:01 PM (16 years, 3 months ago)

What signs do you see? I had the jars laying on their sides when this spot showed up. Its only below the liquid for the picture.


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Re: Purple Contam. [Re: legallyhomeless]
    #7707612 - 12/02/07 05:53 PM (16 years, 3 months ago)

Bacterial contamination will give the entire mycelium a 'wet look'. It will be wet and shiny, like yours. I sometimes refer to it as the wet ice cream look. The purple spot might also be yeast.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Re: Purple Contam. [Re: RogerRabbit]
    #7707642 - 12/02/07 05:59 PM (16 years, 3 months ago)

Not saying your wrong, but when I was trying to get a decent photo I turned it around several times. The liquid you see in the picture was ran all over the myc before a clear picture was taken. Possibly giving it the wet look you see.


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Re: Purple Contam. [Re: legallyhomeless]
    #7708197 - 12/02/07 07:58 PM (16 years, 3 months ago)

Give it the smell test. Either way, I'd toss it.
RR


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semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Purple Contam. [Re: RogerRabbit]
    #7708509 - 12/02/07 09:16 PM (16 years, 3 months ago)

Alright. Thanks


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