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Offlinesproket13
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can trich live dormant
    #7707789 - 12/02/07 06:26 PM (16 years, 1 month ago)

ok. so after trying to make 2 serperate batches of jars. both getting trich while growing. ive realized its coming from my LC's which are disease free. but then it must be when im using my syringe to suck up the LC. somewhere there im getting contams.



now. ive never had contams in any of my WBS jars. but eventually they will get trich it seems. after like a month or so in the FC.


its odd that my jars get trich. but following the same procedures, my wbs doesnt. OR DOES IT? can trich be there and not blossom right away???







im going crazy trying to understand why my jars keep getting trich in them!!!


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OfflineNibin
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Re: can trich live dormant [Re: sproket13]
    #7709234 - 12/03/07 12:08 AM (16 years, 1 month ago)

how do you know your LC is trich free?


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OfflineThe shroomy 1
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Re: can trich live dormant [Re: Nibin]
    #7709645 - 12/03/07 04:42 AM (16 years, 1 month ago)

Now that is the million dollar question! I've often wondered the same damn thing! Why does Trich show up after a month. I guess I should be thankful that 1. My spores where contam free. 2. My agar was contam free. 3. my WBS procedure was contam free. 4. My spawning to bulk substrate was contam free. 5. I GOT AT LEAST 3 FLUSHES FROM MY GROW!. But it never fails, Trich comes in at some point that clues me in to the fact that its all over. Where did it come from? Was it there all the time? Good Question.


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OfflineThe shroomy 1
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Re: can trich live dormant [Re: Nibin]
    #7709648 - 12/03/07 04:44 AM (16 years, 1 month ago)

Now that is the million dollar question! I've often wondered the same damn thing! Why does Trich show up after a month. I guess I should be thankful that 1. My spores where contam free. 2. My agar was contam free. 3. my WBS procedure was contam free. 4. My spawning to bulk substrate was contam free. 5. I GOT AT LEAST 3 FLUSHES FROM MY GROW!. But it never fails, Trich comes in at some point that clues me in to the fact that its all over. Where did it come from? Was it there all the time? Good Question.


--------------------


AMU Q&A thread.


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Offlineveda_sticks
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Re: can trich live dormant [Re: The shroomy 1]
    #7709677 - 12/03/07 05:22 AM (16 years, 1 month ago)

Most Mould mycelium is white, you usually only notice it when it sporolates, its quite possible that it has been there all along and you just didnt see it.

LC's are really hard to spot contams in. As far as i know, the only indication is a cloudy looking water. Contams dont show them selfs in LC's.

As for trich after several flushes, i think thats what generally happens as your project gets older,


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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InvisibleSillicybin
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Re: can trich live dormant [Re: veda_sticks]
    #7709972 - 12/03/07 08:55 AM (16 years, 1 month ago)

If trich is in a grain jar, it will start growing and spreading VERY quickly, usually contaminating the whole jar in less than 5 days. The thing is, it actually has to land on the grain and germinate.... if there's a spore sitting on the inside of the jar (not contacting the grains) and a movement or a shake allows the spore to contact the grain, it can be a delayed infection. If this happens, it's probably not your LC, but more likely your inoculation procedure or your gas exchange filter.


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OfflineRogerRabbitM
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Re: can trich live dormant [Re: Sillicybin]
    #7710006 - 12/03/07 09:18 AM (16 years, 1 month ago)

Trich won't sporulate in an LC, thus you have no way of knowing that your culture is contaminated. That's one of the reasons I think the whole LC craze is silly, and I've long advised against them. Lots of growers waste valuable time growing molds instead of mushroom mycelium, and don't even know it for months, thus all their work is ruined.

If you haven't learned agar skills yet, I'd suggest injecting spores into grains, where you'll see molds within days if they're present. If your jar fully colonizes mold free, you can use one jar to inoculate ten to twenty more, thus expanding your mycelium much farther and faster than an LC made from a spore syringe ever could.
RR


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OfflineWronguy

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Re: can trich live dormant [Re: RogerRabbit]
    #7710036 - 12/03/07 09:33 AM (16 years, 1 month ago)

Agreed on not using an LC for multi-spore or untested grows. I always recommend isolating on agar and than making that transfer to your selected media. I personally do not discount liquid cultures though. I have always used them since I started sterilizing my substrates.

I can't simply open my bag and add spawn unless I'm in front of my flow-hood, so a liquid culture becomes paramount in this situation.


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OfflineRogerRabbitM
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Re: can trich live dormant [Re: Wronguy]
    #7710053 - 12/03/07 09:40 AM (16 years, 1 month ago)

Agreed, LC can have its place when made properly under controlled conditions. I use a proved strain on a petri dish, and eberbach it into an LC for inoculations six days later at most. However, this procedure is out of reach for those without agar skills, a microscope to verify a clean culture, an eberbach autoclavable blender, and a flowhood.

What I hate is the constant advice from noobs to other noobs to inject a spore syringe into karo water and hope for the best. I've seen many new growers around here give up after a few tries with LC and lose interest in the hobby. If they had simply followed established procedure, they would have grown mushrooms instead of molds.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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OfflineWronguy

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Re: can trich live dormant [Re: RogerRabbit]
    #7710070 - 12/03/07 09:45 AM (16 years, 1 month ago)

:thumbup:


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Offlineveda_sticks
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Re: can trich live dormant [Re: Wronguy]
    #7710722 - 12/03/07 12:29 PM (16 years, 1 month ago)

I agree with you rr, and I have been guilty of that on a few occassions based on very few uses of lc.

I know know that its the not the best route to go for the beginer.

but it is nice to grow an lc from spores when u can guarentee sterility. if you prefer to keep it really simple and don't want to go agar and have to buy lots of different spores.

I like lc,s as its convenient, not got much time to be making agar and isolating etc (which I would like to try)

if an lc for me gets contammed then I always have something from multispore just incase.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflineNibin
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Re: can trich live dormant [Re: RogerRabbit]
    #7711163 - 12/03/07 02:10 PM (16 years, 1 month ago)

Quote:

RogerRabbit said:
Agreed, LC can have its place when made properly under controlled conditions. I use a proved strain on a petri dish, and eberbach it into an LC for inoculations six days later at most. However, this procedure is out of reach for those without agar skills, a microscope to verify a clean culture, an eberbach autoclavable blender, and a flowhood.

What I hate is the constant advice from noobs to other noobs to inject a spore syringe into karo water and hope for the best. I've seen many new growers around here give up after a few tries with LC and lose interest in the hobby. If they had simply followed established procedure, they would have grown mushrooms instead of molds.
RR




I'd make a LC from a vendor syringe but never with one of my own.


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Newcomers guide-----> For all things shroomy


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Offlinesproket13
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Re: can trich live dormant [Re: Nibin]
    #7711219 - 12/03/07 02:24 PM (16 years, 1 month ago)

so within an LC...trich can live dormant.  alright. i have about 7 different LC's that ive made at random. not needing them, just letting them grow. i think im gonna make a lot of BRF jars(that is my goal here...brf cakes) and then take 30cc syringes, fill up each one w/ a different LC. then inject just 1 jars with each. if it grows clean, i know my LC is good. and the transfer to the syringe was good.


my LC's look good. but as RR said, it can be there, youll never know! 


5 days ago i ordered 100 petri dishes  :laugh:


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Offlineveda_sticks
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Re: can trich live dormant [Re: sproket13]
    #7711557 - 12/03/07 03:56 PM (16 years, 1 month ago)

With an LC always just test on j1 ar. Just in case, would be annoying to loose a whole bacth


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
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