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Offlineextramadchicken
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Registered: 11/11/07
Posts: 21
Last seen: 7 years, 7 months
not working?
    #7706427 - 12/02/07 12:30 PM (16 years, 1 month ago)

Hi this is my first real attempted grow and i am having some problems. I prepared 4 jars with verm and brown rice flour and followed the begginers guide. It has been a week since i inoculated the jars and i see nothing. I followed every step correctly, except that i had to use a sterile dropper instead of a syringe because i cant get syringes in my state. Also, could it be that since it has been cold in my house the growth is slowed?


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OfflineBanjoMojo
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Registered: 09/29/07
Posts: 148
Loc: Alabama-ish
Last seen: 4 years, 4 months
Re: not working? [Re: extramadchicken]
    #7706471 - 12/02/07 12:43 PM (16 years, 1 month ago)

Temperature may be part of the problem. You'll want a temperature in the neighborhood of 78-80 for colonization.

Also, with a multispore inoculation, it can takes from 7-10 days before you see any growth at all. On the other hand, if you use a liquid culture (LC), you see growth much more quickly because mycelia have already been formed.

Be patient and make sure your temp is right. Post pictures as you see changes!


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:firefox: If God is inside us like some people say, He'd better like burritos 'cause that's what he's getting. :firefox:

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Offlineextramadchicken
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Registered: 11/11/07
Posts: 21
Last seen: 7 years, 7 months
Re: not working? [Re: BanjoMojo]
    #7706629 - 12/02/07 01:19 PM (16 years, 1 month ago)

thankyou for the help i think im gonna move my jars to my friend's house which is much warmer. He can afford to have his heater on all day unlike me.


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OfflineBlargIAmDead
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Registered: 11/04/07
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Re: not working? [Re: extramadchicken]
    #7708578 - 12/02/07 09:36 PM (16 years, 1 month ago)

So wait....did you drop the multi-spore solution through the holes onto the top of the dry verm layer? Cause that is a prime situation for contamination wicking. And you can get syringes in any state, order them online. If you have a store that sells printers/refill kits for printer cartridges in your state...you have syringes that are FANTASTIC for this hobby.


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OfflinePr0_X
CultivationLifer
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Registered: 01/18/06
Posts: 617
Loc: Igloo's and polar bears
Last seen: 16 years, 1 day
Re: not working? [Re: BlargIAmDead]
    #7708629 - 12/02/07 09:50 PM (16 years, 1 month ago)

Did you use a dry vermiculite air to filter airborn contamination? and if so when you inoculated was the spore solution in the middle of the glass?

you can see growth in 3-10 days at 72f, higher temp's favor bacteria.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

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