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OfflineCubeGod666
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Registered: 11/24/07
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No Glove Box No Problem?
    #7705998 - 12/02/07 12:13 PM (13 years, 5 months ago)

Alright guys, I've got a 10cc spore syringe from spores 101 and 12 mushbox substrate jars on the way. What I don't have is a glove box, nor do I feel like building or buying one.

I'd be willing to try the oven tek if anyone could break it down for me?
If not, should I be ok just torching the tip of the spore syringe to glowing red before inoculation.


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OfflineNibin
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Re: No Glove Box No Problem? [Re: CubeGod666]
    #7706029 - 12/02/07 12:24 PM (13 years, 5 months ago)

oven tek is a no no, as it will create air currents which we want to avoid.

Find a place with still air. Wash down all surfaces. Cover hair and mouth and wear gloves.

Then flame needle till red hot, wipe down with some alcohol soaked paper/gauze and immediately inoculate the jars through the holes, without letting the needle touch the table.

Repeat the flaming and wipe down between jars to avoid cross contamination.


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Newcomers guide-----> For all things shroomy


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OfflineMolasses
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Re: No Glove Box No Problem? [Re: CubeGod666]
    #7706032 - 12/02/07 12:25 PM (13 years, 5 months ago)



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Invisiblenorml840
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Re: No Glove Box No Problem? *DELETED* [Re: Molasses]
    #7706050 - 12/02/07 12:35 PM (13 years, 5 months ago)

Post deleted by norml840

Reason for deletion: bad advice



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OfflineNibin
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Re: No Glove Box No Problem? [Re: norml840]
    #7706073 - 12/02/07 12:45 PM (13 years, 5 months ago)

And I've inoculated over 50 jars with no contams in my dirty sitting room.

But working in the oven has become a thing of the past as people realized that you were doing more harm than good by producing the air currents, not to mention having to mess about trying to avoid hot elements if your oven is electric.

As long as you flame sterilize and wipe down with alcohol (i don't even do that i just squirt out some solution) you should have no problems especially if you have no air currents in the area you are working in and cover your mouth and hair, both of which can cause currents and harbour contams.


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Newcomers guide-----> For all things shroomy


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OfflineCubeGod666
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Re: No Glove Box No Problem? [Re: Nibin]
    #7706093 - 12/02/07 12:53 PM (13 years, 5 months ago)

What if I use a lidless rubbermaid, sterilized with bleach water,
I wash my hands, place the jars in the rubbermaid, and wipe the jars down with alcohol pads. Then wipe the needle down with alcohol wipes, flame it to glowing red, and then inocculate the jars.

Would that tremendously reduce my risk of contams?

I can't afford another contam problem, I need a crop.
I've had two trys go to shit, so now I invested some money in it.


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Offlineurayasan
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Re: No Glove Box No Problem? [Re: CubeGod666]
    #7706158 - 12/02/07 01:13 PM (13 years, 5 months ago)

Sorry to lay it on you but if you genuinely "can't afford" to have failure, and obviously have access to a rubbermaid, just do it the right way and you will significantly increase your chances of success. Don't be lazy. It's not that hard. Maybe you are overestimating the effort it takes to make one, it's a very minor investment of time that will pay off for as long as you make use of it.

Good luck :snowflake:


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OfflineNibin
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Re: No Glove Box No Problem? [Re: urayasan]
    #7706250 - 12/02/07 01:43 PM (13 years, 5 months ago)

Use a pair of gloves, just thin latex ones and wipe em down with alcohol.

Shower before innoculating, then cover hair and mouth with something, and wear clean clothes.

Close all windows, you don't want draught and use a rubbermaid or transparent trash bag to inoculate in.

DO NOT WIPE DOWN NEEDLE WITH ALCOHOL BEFORE FLAME STERILIZING, alcohol fumes + flame = fire. First heat it till red hot THEN wipe down with alcohol and inoculate.


--------------------
Newcomers guide-----> For all things shroomy


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OfflineSubstance D
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Re: No Glove Box No Problem? [Re: Nibin]
    #7706536 - 12/02/07 02:58 PM (13 years, 5 months ago)

Quote:

Nibin said:
Use a pair of gloves, just thin latex ones and wipe em down with alcohol.

Shower before innoculating, then cover hair and mouth with something, and wear clean clothes.

Close all windows, you don't want draught and use a rubbermaid or transparent trash bag to inoculate in.

DO NOT WIPE DOWN NEEDLE WITH ALCOHOL BEFORE FLAME STERILIZING, alcohol fumes + flame = fire. First heat it till red hot THEN wipe down with alcohol and inoculate.


could you explain more about the trash bags do you but all the jars in there then inject them while there in the bag an why transparent,im gonna make my first attempt later this weak just waiting on my jars to come


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OfflineNibin
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Re: No Glove Box No Problem? [Re: Substance D]
    #7706701 - 12/02/07 03:40 PM (13 years, 5 months ago)

use the search function and put in trash bag glove box as your search words.

There have been many posts explaining this in the past


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Newcomers guide-----> For all things shroomy


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OfflineHer0
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Re: No Glove Box No Problem? [Re: CubeGod666]
    #7706955 - 12/02/07 04:51 PM (13 years, 5 months ago)

Quote:

CubeGod666 said:
What if I use a lidless rubbermaid, sterilized with bleach water,
I wash my hands, place the jars in the rubbermaid, and wipe the jars down with alcohol pads. Then wipe the needle down with alcohol wipes, flame it to glowing red, and then inocculate the jars.

Would that tremendously reduce my risk of contams?

I can't afford another contam problem, I need a crop.
I've had two trys go to shit, so now I invested some money in it.





If you already have a rubber maid.. turn it into a cheap ass glovebox by putting it on its side with all your stuff in it.. seal the open end with seran wrap or a clear garbage bag taped tightly.. cut two holes for your arms to reach through.. clean your arms, and wipe it all down. Let your alcohol or w/e u used to sterilize evaporate then get'r done.

A glove box doest have to be technical.. the less open air, the less chance of contams landing on your project. Just isolate and sterilize as best you can.


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OfflineMYSTIQUE
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Re: No Glove Box No Problem? [Re: Her0]
    #7706986 - 12/02/07 04:59 PM (13 years, 5 months ago)

I use big clear garbage bags and I just have to push the top of it up every once and a while and hope it done melt threw but it does the job and I can just leave all my jars when I'm done so they can just incubate in it.


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:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:


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Offlinepunkhardcore92
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Re: No Glove Box No Problem? [Re: MYSTIQUE]
    #7707568 - 12/02/07 07:44 PM (13 years, 5 months ago)

I've done it right here in my room without a glove box before just out of sheer lazyness. It still worked out but thats back when i had more Myc then poo to introduce it with and didnt really care casue i didnt need more


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Edited by punkhardcore92 (12/02/07 07:44 PM)


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Offlineveda_sticks
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Re: No Glove Box No Problem? [Re: punkhardcore92]
    #7707706 - 12/02/07 08:11 PM (13 years, 5 months ago)

I have used the oven tek before and had a 90% failure rate. as soon as I moved to using a glovebox I have had none so far.

the kitchen is the worst place. its full of all sorts of moulds and bacteria. the oven ist exactly clean either.

a glove bos can be made pretty cheap. even a cardboard box with holes cut out for your arms and a clear lid taped to the top will do. if u have some plexi glass that's perfect for the lid taped to the box. if not I guess evgen cling film will work.

a glove box is less work, than having to try and disinfect n entire area.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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