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OfflinePoisonedV
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Agar Cultures
    #7704545 - 12/01/07 09:54 PM (16 years, 2 months ago)

OK, I cloned some cultures. Now, how do I actually inoculate a jar with it?


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Invisiblejeetered
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Re: Agar Cultures [Re: PoisonedV]
    #7704597 - 12/01/07 10:11 PM (16 years, 2 months ago)

take a wedge to grain, then do grain to grain transfers. Be sure to set it myc side against the grain, not the agar side down.


Edited by jeetered (12/01/07 10:12 PM)


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Offlineveda_sticks
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Re: Agar Cultures [Re: jeetered]
    #7705561 - 12/02/07 04:55 AM (16 years, 1 month ago)

Like jeetered said, or you can transfer a small scraping the size of a grain of rice, from the newly grown myc into and LC, grow that out as your master culture, take a syringe of the myc water, inoc a few more jars to use as working culture then inoc your substrate.

Store the master culture in the fridge,


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InvisiblePrisoner#1
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Re: Agar Cultures [Re: veda_sticks]
    #7705566 - 12/02/07 05:03 AM (16 years, 1 month ago)

or alternately once you've decided you like the strain and it's ready for
archiving you can prepare some slants, transfer a bit of mycelium to the slant,
allow it to incubate a few days, top it with distilled water and stick it in the
deep freeze

I usually keep about 20 of each culture I like in this manner for long term storage


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