Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Original Sensible Seeds High THC Strains   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinesproket13
Stranger
Male


Registered: 10/26/05
Posts: 571
Loc: FL
Last seen: 10 years, 7 months
contam question
    #7702256 - 12/01/07 10:23 AM (16 years, 2 months ago)

so recently. ive tried making 2 batches of jars. and both got contaminated. the jars were used before. but i cleaned them in a dishwasher, then cleaned them with alcohol before mixing up the BRF/verm and putting itin there. i have a barrier layer...all my cakes have worked before, until now! every cake gets trich where it was injected. im using mycelium from an LC i have, thats not contamed. ive used the LC with WBS and had no problems.


so im wondering if any contams could survive a 1.5 hour 15 psi pressure cooking. it makes no sense, i have a still air box. flame my needle between jars. yet everyone contams the same way. wtf!!!


anyone have any ideaS?


Extras: Filter Print Post Top
Offlinedoc34
Fungitarian
Male User Gallery


Registered: 02/14/04
Posts: 2,667
Loc: Myceliaville !!!
Last seen: 7 months, 29 days
Re: contam question [Re: sproket13]
    #7702265 - 12/01/07 10:30 AM (16 years, 2 months ago)

Quote:

so im wondering if any contams could survive a 1.5 hour 15 psi pressure cooking




Highly unlikely.

I would say that somewhere between your lc and your inoculation, lies your troubles.


--------------------


Extras: Filter Print Post Top
InvisibleNwerd
Stranger
Male User Gallery

Registered: 10/20/07
Posts: 259
Re: contam question [Re: doc34]
    #7702425 - 12/01/07 11:15 AM (16 years, 2 months ago)

Wiping down the inoc spots with alcohol is necessary prior to each injection if not each jar...
This might be the culprit?
Think how many tiny mold spores could be just waitin for your needle to hitch a rid....
Not sure if you did this, but it's essential


Extras: Filter Print Post Top
Offlinesproket13
Stranger
Male


Registered: 10/26/05
Posts: 571
Loc: FL
Last seen: 10 years, 7 months
Re: contam question [Re: Nwerd]
    #7702441 - 12/01/07 11:21 AM (16 years, 2 months ago)

good point. i only wipe the jar lids with alcohol before all the material goes in. not right before inoculation. but man, i highly doubt that EVERY jar had the same problem!!!! 


its driving me crazy!  no way this is normal  :frown:

never had trich problems before. not atleast until the FC..


Extras: Filter Print Post Top
InvisibleNwerd
Stranger
Male User Gallery

Registered: 10/20/07
Posts: 259
Re: contam question [Re: sproket13]
    #7702456 - 12/01/07 11:26 AM (16 years, 2 months ago)

Yeah that's a quandary my friend...
You'd be surprised how tenacious those mold spores can be though...
The one time I got lazy and did not wipe the jar tops...
I had all of em contam and had to dump em...
Probably a few other factors play a role, but I'm guessing that was the main source of infection...
1.5 hours at 15psi is the sweet spot... You're substrate should have been fine...


Extras: Filter Print Post Top
Offlinesproket13
Stranger
Male


Registered: 10/26/05
Posts: 571
Loc: FL
Last seen: 10 years, 7 months
Re: contam question [Re: Nwerd]
    #7702633 - 12/01/07 12:24 PM (16 years, 2 months ago)

man. ill prepare some more jars tomorrow. maybe this time i wont make 18 tho. lmao.  i thought for SURE this time i wouldnt have a problem. lol...they were brand new jar lids. i just drilled out

i baked my verm layer at 350 for 2 hours (forgot about it..) so i really know everything should have been fine!!


im having a hard time thinking its because i didnt wipe down my jar lids.  the needle doesnt even really touch it if your a good shot  :smile:


Extras: Filter Print Post Top
OfflineBlargIAmDead
Shroom Samurai
 User Gallery


Folding@home Statistics
Registered: 11/04/07
Posts: 550
Last seen: 14 years, 11 months
Re: contam question [Re: sproket13]
    #7702653 - 12/01/07 12:29 PM (16 years, 2 months ago)

Some of us (me) have issues with shaking. And I don't mean small shivers, we're talking cerebral palsy level shaking whenever I try to do anything delicate. Have you tried moving your glovebox/workarea to a different part of your dwelling/abode?


Extras: Filter Print Post Top
Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: contam question [Re: sproket13]
    #7702654 - 12/01/07 12:29 PM (16 years, 2 months ago)

It could just be a run of bad luck, i have never baked the verm layer. Pressure cooker is more than enough to sterise the contents of the jar.

You can try wiping your jar lids down more, but i dont think that would be the problem, as you should have foil on your lids, which u dont take of till you are ready to inoc.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
Offlinesproket13
Stranger
Male


Registered: 10/26/05
Posts: 571
Loc: FL
Last seen: 10 years, 7 months
Re: contam question [Re: veda_sticks]
    #7702700 - 12/01/07 12:44 PM (16 years, 2 months ago)

all my inocculations are done in my bathroom. 4 days ago, knocked up 4 jars of WBS and they are almost 100% just 2 days ago i transfered some WBS from jars to containers, today mycelium fuzz is breaking through already.


so why the hell are my jars going to shit! i even used a new needle last time.


i am using LC for inoculation. could something be going wrong from that??


the LC i used. was a koh sumai fruit clone. i made 1 master, then a month later made 2 more LC's one using honey, the other karo. the karo is fine, the honey got contammed (first time in an LC) now i tried using the koh to knock up jars, and it got contam. i figured the LC was bad, so i tried another LC from a multispore of MAZZ. still got the green. drivin me nuts!


in the same incubation chamber, is my WBS casings which are contam free.


Extras: Filter Print Post Top
OfflineNibin
Getting there
Male User Gallery


Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
Trusted Cultivator
Re: contam question [Re: sproket13]
    #7702879 - 12/01/07 01:48 PM (16 years, 2 months ago)

lots of black mold in bathrooms...


--------------------
Newcomers guide-----> For all things shroomy


Extras: Filter Print Post Top
InvisibleOptx
PronouncedAwp-Tiks
 User Gallery

Registered: 03/28/05
Posts: 977
Re: contam question [Re: sproket13]
    #7703009 - 12/01/07 02:34 PM (16 years, 2 months ago)

yeah, i would never do anything sterile in my bathroom. ever. and my guess for your contam problems (if not the restroom) would be the syringe used for sucking up your clean LC hasn't been completely sterilized. just my guess, but it seems to make sense if the LC really is clean and you're getting trich only on the inoculation sites.


--------------------
please do not take everything i say here so personally. welcome to teh internets!


Extras: Filter Print Post Top
Offlinesproket13
Stranger
Male


Registered: 10/26/05
Posts: 571
Loc: FL
Last seen: 10 years, 7 months
Re: contam question [Re: Optx]
    #7703075 - 12/01/07 02:59 PM (16 years, 2 months ago)

my house is under 2 years old.  granted its not safe from everything. i spray oust in the room. turn off the AC. and use a still air box...

and yah. i thought after my first mess up it was the syringe suckin up the mycelium. so this last time, i sucked up alcohol, and let it sit for 2 hours. then boiling water through it. then sucked up the mycelium.  im sure thats sterile :crazy2:


Extras: Filter Print Post Top
OfflineCheesekiller
Mad Scientist
Male


Registered: 11/08/04
Posts: 485
Loc: Central NY
Last seen: 1 month, 1 day
Re: contam question [Re: sproket13]
    #7703165 - 12/01/07 03:24 PM (16 years, 2 months ago)

Gotta be the LC. Same thing happened to me with the Koh Sumai super strain. My LC looked fine, but my WBS got F'ed. I find using a print the best start. Print to syringe, then hydrate for 24hrs, and you have a shotgun. I am curious though why are you making cakes when you got a good WBS operation going?? That's all I use for spawn.

-CK


--------------------
Bulk grower with "some" success.

  Cloning Machine 
   
Nice Lids

A few pics of my DT setup


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate   Original Sensible Seeds High THC Strains   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Contam Question BlipKorterly 742 1 08/03/02 01:33 AM
by paranoid2
* contam question nightbreed 877 6 05/28/01 04:20 AM
by auto59009
* contam question GaHippy 679 1 02/27/02 12:39 AM
by Zorba
* Re: Contam Question tom 644 2 07/03/00 10:14 AM
by Krupa
* Contam? Question... Mithril 456 1 11/27/02 09:53 AM
by Bi0TeK
* mycelium contam and fruiting time nathancd 1,683 1 06/07/01 05:04 AM
by puscle
* NJ-6 problem strain 768 6 01/29/02 10:25 PM
by repobob
* question for ya fla 341 1 10/14/02 09:24 PM
by Skikid16

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,421 topic views. 18 members, 260 guests and 61 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.009 seconds on 14 queries.