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OfflineGWAR
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Myceluim-water syringe from PF cake
    #7699727 - 11/30/07 04:16 PM (16 years, 2 months ago)

I'm editing this because I think I over explained it.

basically, all I want to know is..

can i put a generous amount of live mycelium from a PF cake into a jar with sterilized water, shake it, and use this to inoculate a rye bag?

thanks.




original post:
--------------------

I have two 100% colonized jars that have probably been ready to go for a few weeks now, I just haven't had time to do anything worth while with them. They are both very very white and healthy looking.

  • My plan is to take half a cake and put it in a a pint jar full of sterile water.
  • Then I'm going to seal this jar containing the PF cake and water mixture, and shake it well to disperse the mycelium.
  • After that I'll poke a small hole and fill a sterilized (used)syringe with the mycelium water I have just made. I will then put tape over the hole i made, and maybe put tinfoil over the top too, and keep this jar of PF cake mycelium water to possibly make future mycelium syringes from.
  • Finally, I'm going to inoculate a couple sterilized rye bags with the syringe I have just made.


Will this work?
Or would it be better just to cut open the bags and mix in the PF jar?


Edited by GWAR (11/30/07 04:29 PM)


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Invisiblefee
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Re: Myceluim-water syringe from PF cake *DELETED* [Re: GWAR]
    #7699996 - 11/30/07 05:17 PM (16 years, 2 months ago)

Post deleted by fee

Reason for deletion: ...



--------------------

blankk said to fee:
btw you're a total fucking psychedelic pimp
Turtletotem said:
I want to become a sun worshipper, so next time an atheist smugly asks me where god is, I can point smugly at the sun and laugh my ass off.

Then I drive away in my solar powered piece of shit car, cool stuff man.

And then I go kill a bitch because the flaming orb in the sky told me to do so, and I don't know, oppress a few minorities here and there in the name of nuclear fusion?

Religion is fun.


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OfflinePr0_X
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Re: Myceluim-water syringe from PF cake [Re: fee]
    #7700036 - 11/30/07 05:26 PM (16 years, 2 months ago)

I never had any luck with the mycelium syringe tek either, always failed for me but it's worked for others, you'll have to try it to find out.


--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

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OfflineGWAR
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Re: Myceluim-water syringe from PF cake [Re: Pr0_X]
    #7700043 - 11/30/07 05:28 PM (16 years, 2 months ago)

Ok... I guess it's worth a shot

anyone else tried this tek with any success?
http://www.shroomery.org/65/Uncle-Lazlos-EZ-Mycelium-Syringe-Tek


--------------------

"Freedom to all the people... Brave, true and strong... Freedom to all the people... Unless I think you're wrong!!!"


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OfflineRogerRabbitM
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Re: Myceluim-water syringe from PF cake [Re: Pr0_X]
    #7700054 - 11/30/07 05:30 PM (16 years, 2 months ago)

Making mycelium syringes works much better with grains than with brf. You can do it though. Be sure to inject and draw water from far below the vermiculite barrier, since that's where the contamination is.

Once you've removed it from the jar, the cake is no longer sterile, so you wouldn't want to use it to inoculate grains. It can inoculate any bulk substrate however.
RR


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OfflineGWAR
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Re: Myceluim-water syringe from PF cake [Re: RogerRabbit]
    #7700079 - 11/30/07 05:35 PM (16 years, 2 months ago)

RogerRabbit,

is the best method of getting a mycelium syringe from a PF jar is to inject the jar with sterilized water... then to redraw this water back into the syringe? or no?


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"Freedom to all the people... Brave, true and strong... Freedom to all the people... Unless I think you're wrong!!!"


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OfflineGWAR
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Re: Mycelium-water syringe from PF cake [Re: GWAR]
    #7700111 - 11/30/07 05:45 PM (16 years, 2 months ago)

Maybe I'll just skip all that shit and inject the 3lb rye bags with spores syringes.. I am still undecided tho :confused:


--------------------

"Freedom to all the people... Brave, true and strong... Freedom to all the people... Unless I think you're wrong!!!"


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