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Pentahedral
Stranger
Registered: 10/23/07
Posts: 16
Last seen: 16 years, 1 month
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Cloning mycelium question
#7698305 - 11/30/07 10:39 AM (16 years, 2 months ago) |
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Okay, bear with my since I'm a noob at this, and as well trying to do it on a very limited budget.
I've got 4 pint jars and 1 half pint jar fully colonized with B+. I would like to get into bulk substrates but for now all I have for substrate is BRF. I was thinking that it would be the easiest to case the 3 pint jars, and that leaves the half pint jar left. I'm wondering what would the easiest/quickest/best way to clone or transfer the mycelium into roughly 10 pint jars.
I've read all the various teks but am kinda lost. I could go with mycelium water solution, but then I would have to find a source of needles. The other way I was thinking would be to sterilize say ten jars in the PC, then dump all of those, plus the colonized jar into an oven bag with a tyvek filter for FAE, but that seems to leave a lot of things open to contamination. Or I could sterilize the jars and substrate, then dump everything into a bowl, mix, then put back into jars but I have no flowhood/glovebox so that would be extremely risky...
Basically any input is welcome, except quit now and start over!  Thanks!
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BlargIAmDead
Shroom Samurai




Registered: 11/04/07
Posts: 550
Last seen: 14 years, 11 months
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Re: Cloning mycelium question [Re: Pentahedral]
#7699269 - 11/30/07 02:16 PM (16 years, 2 months ago) |
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Just fruit the BRF cakes in your FC and clone a fruit O.o. Have a little patience. What you're talking about doing is kinda a modified grain to grain. And most people here don't use BRF as a bulk substrate.
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Pentahedral
Stranger
Registered: 10/23/07
Posts: 16
Last seen: 16 years, 1 month
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Re: Cloning mycelium question [Re: BlargIAmDead]
#7700132 - 11/30/07 05:49 PM (16 years, 2 months ago) |
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Quote:
BlargIAmDead said: Just fruit the BRF cakes in your FC and clone a fruit O.o. Have a little patience. What you're talking about doing is kinda a modified grain to grain. And most people here don't use BRF as a bulk substrate.
I've had luck doing this before, a couple weeks ago I took a half pint jar and dumped it into a bowl with 3 pints of sterilized BRF, mixed, then put back into 3 pint jars with polyfil. So far the jars are ~95-97% colonized with no contamination.
I believe using 1-3% HP added to the substrate when I mix it would get even better results since the mycelium would not be affected. And yes, I know people don't usually use BRF as bulk substrate but I would that's what I got to work with so I have to make it work. I'm planning on casing the pint jars, I just need a better way to get ~15 pint jars so I have at least some spare mycelium if things go bad.
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Pentahedral
Stranger
Registered: 10/23/07
Posts: 16
Last seen: 16 years, 1 month
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Re: Cloning mycelium question [Re: Pentahedral]
#7706188 - 12/02/07 11:24 AM (16 years, 1 month ago) |
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anyone?
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djmaloney_aries
Stranger


Registered: 02/21/14
Posts: 25
Last seen: 9 years, 9 months
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Re: Cloning mycelium question [Re: Pentahedral]
#19722959 - 03/20/14 10:06 AM (9 years, 10 months ago) |
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I was curious about this as well. What is the best way to perpetually grow. I would just like to keep a continual supply of shrooms growing. Can this be achieved by just breaking up 1 jar of colonized mycelium among 10 jars of new sterile substrate? And continue this process every month to keep a constant supply of jars colonized?
Thanks!
Edited by djmaloney_aries (03/20/14 10:06 AM)
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alex591955
i feel funny



Registered: 02/17/10
Posts: 672
Last seen: 3 years, 3 months
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Re: Cloning mycelium question [Re: Pentahedral]
#19723129 - 03/20/14 10:40 AM (9 years, 10 months ago) |
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Quote:
Pentahedral said: Okay, bear with my since I'm a noob at this, and as well trying to do it on a very limited budget.
I've got 4 pint jars and 1 half pint jar fully colonized with B+. I would like to get into bulk substrates but for now all I have for substrate is BRF. I was thinking that it would be the easiest to case the 3 pint jars, and that leaves the half pint jar left. I'm wondering what would the easiest/quickest/best way to clone or transfer the mycelium into roughly 10 pint jars.
I've read all the various teks but am kinda lost. I could go with mycelium water solution, but then I would have to find a source of needles. The other way I was thinking would be to sterilize say ten jars in the PC, then dump all of those, plus the colonized jar into an oven bag with a tyvek filter for FAE, but that seems to leave a lot of things open to contamination. Or I could sterilize the jars and substrate, then dump everything into a bowl, mix, then put back into jars but I have no flowhood/glovebox so that would be extremely risky...
Basically any input is welcome, except quit now and start over!  Thanks!
Your wanting to take a brf jar and g2g it to a different jar. Use the search function and search for "g2g brf" I found lots when i did.
Your also going to "spawn" your brf jars, not casing. Casing is a layer of say like coir/verm you apply to when your mono sub is 100% colonized.
YOur going to most likely get a contaim doing what you said you wanted.
Do you still have any spores that you used in the begining?
Also G2G is going to be the best way to keep things going.
-------------------- My Current Grow
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djmaloney_aries
Stranger


Registered: 02/21/14
Posts: 25
Last seen: 9 years, 9 months
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Re: Cloning mycelium question [Re: alex591955]
#19724759 - 03/20/14 04:12 PM (9 years, 10 months ago) |
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Yes, I have plenty of spores now. But it seams to me that once I get a good strain going that I should be able to continue to use that good mycelium to inoculate more jars. This should cut a week off the colonizing period, as well as prevent me from having to keep buying more spores or maintain prints until such time I want to stop growing. Sorry, I am not up on all the terminology, can make getting proper search results a bit daunting at times.
Thanks!
Edited by djmaloney_aries (03/20/14 04:15 PM)
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 18 days, 9 hours
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Fellas, this thread was from 6 years ago..
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djmaloney_aries
Stranger


Registered: 02/21/14
Posts: 25
Last seen: 9 years, 9 months
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Re: Cloning mycelium question [Re: Edmunter]
#19727821 - 03/21/14 11:18 AM (9 years, 10 months ago) |
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Yeah, but I am new to the hobby and this forum. I suppose that once I learn some of the commonly used terms, searching will become easier. Maybe someone could put together a list of acronyms and terms for us NoObs.
Just found out today what GTG means as it relates to this subject.
Thanks!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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you search the terms to find them out. and then you can search with those terms to find more out.
 inception
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djmaloney_aries
Stranger


Registered: 02/21/14
Posts: 25
Last seen: 9 years, 9 months
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Re: Cloning mycelium question [Re: bodhisatta]
#19727943 - 03/21/14 11:48 AM (9 years, 10 months ago) |
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Great links! I will read them all... Especially on GTG which is what I am most interested in at this point. I have a few jars that should be at 100% in a week or so. I want to keep this particular strain going without isolation as it looks very stringy and promising. I have been growing marijuana for about 3 years now and still learning much with that. I realize that growing mushrooms is very different, yet the concepts are very similar.
Thank you again!
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