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Offlinerichismalls
Richi-smalls


Registered: 10/19/02
Posts: 769
Loc: the ill state
Last seen: 3 months, 15 days
transferring from petri dish to ???
    #7694215 - 11/29/07 09:55 AM (16 years, 2 months ago)

what do you do after that to transfer to something else..i want to clone my PE strain but im not sure what to do after it colonizes the agar.


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InvisibleRoadkillM
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: transferring from petri dish to ??? [Re: richismalls]
    #7694426 - 11/29/07 11:16 AM (16 years, 2 months ago)

lots of things...


transfer some to start another petri dish.

transfer some to a culture tube...
let it colonize a little..
then store it in your frig for later use.

do a transfer to start some jars or bags of substrate.

do a transfer to start an LC jar.


to name a few things.


and you can scrape some of the mycelium off of the petri dish...
put in a sterile vial(or something) with sterile water...
put it in the frig and you will have a culture that will last a very long time.



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinerichismalls
Richi-smalls


Registered: 10/19/02
Posts: 769
Loc: the ill state
Last seen: 3 months, 15 days
Re: transferring from petri dish to ??? [Re: richismalls]
    #7694529 - 11/29/07 11:52 AM (16 years, 2 months ago)

how long does that stay good for generally speaking? do i cut it up into little parts with an exacto knife? does the mycelium usually eat all the agar? can you direct me to a good tek for all of this?


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InvisibleRoadkillM
Retired Shroomery Mod
Male User Gallery


Registered: 12/11/01
Posts: 22,674
Loc: Montana
Trusted Cultivator
Re: transferring from petri dish to ??? [Re: richismalls]
    #7694768 - 11/29/07 01:08 PM (16 years, 2 months ago)

Quote:

richismalls said:

how long does that stay good for generally speaking?







which process?

please be more specific to which one you are asking about.



Quote:

richismalls said:

do i cut it up into little parts with an exacto knife?







when transfering from the agar to something else...

I use an exacto kife...

and you would cut small pie shaped sections out...

like this...





Quote:

richismalls said:

does the mycelium usually eat all the agar?





No

the mycelium only grows on the top surface layer of the agar.



Quote:

richismalls said:

can you direct me to a good tek for all of this?





use the search...

I'm not gonna look them up for you.



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinerichismalls
Richi-smalls


Registered: 10/19/02
Posts: 769
Loc: the ill state
Last seen: 3 months, 15 days
Re: transferring from petri dish to ??? [Re: richismalls]
    #7695142 - 11/29/07 02:53 PM (16 years, 2 months ago)

thanks


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