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North Spore Shop: Injection Grain Bag

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Offlinecosmoline
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Registered: 08/11/07
Posts: 200
Last seen: 5 years, 28 days
LC long term storage containers
    #7695037 - 11/29/07 02:27 PM (16 years, 2 months ago)

I'm looking into getting some containers for storing various LC's. So far the consensus is that you put the mycelium in relatively nutrient free water and keep it cold, maybe a little h202 for contam resistance. Test tubes would be a good call. I would probably make an airport type system. I can't get any test tubes on short notice, what do you guys recommend? Would like to steer away from metal. Thanks.


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Buy the ticket, take the ride.


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Offlinebryanbzl
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Registered: 03/11/07
Posts: 563
Last seen: 5 years, 10 months
Re: LC long term storage containers [Re: cosmoline]
    #7695154 - 11/29/07 02:57 PM (16 years, 2 months ago)

jars... full pint. make a high temp silicone injection port and use tyvek to cover a separate air hole to prevent PC explosion.
All materials can be found at home depot.


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Cheers,

bzl

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"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


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Offlinebryanbzl
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Registered: 03/11/07
Posts: 563
Last seen: 5 years, 10 months
Re: LC long term storage containers [Re: bryanbzl]
    #7695164 - 11/29/07 03:00 PM (16 years, 2 months ago)

baby bottles with tyvek underneath would be an amazing idea aswell.
you can inoculate straight through the rubber nipple.


--------------------
Cheers,

bzl

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------
"From 1898 through to 1910 heroin was marketed as a non-addictive morphine substitute and cough medicine for children."

conclusion:
poor fucking children of the early 1900's.


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OfflineWronguy

Registered: 03/05/05
Posts: 4,450
Last seen: 12 years, 7 months
Trusted Cultivator
Re: LC long term storage containers [Re: bryanbzl]
    #7695323 - 11/29/07 03:34 PM (16 years, 2 months ago)

Vacutainers are always choice number one. I also wouldn't put too much emphasis on what liquid you're using. Simply aspirate some liquid culture, about 6-8 cc, and place the entire solution into a 10 cc vacutainer. Vacutainers cost about $0.10 each.

If you're storing master cultures, such as master isolates, I would recommend using slant jars with agar.


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North Spore Shop: Injection Grain Bag


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