Home | Community | Message Board

MRCA Tyroler Gluckspilze
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineShroomieGirl
What are these god damn animals
Female User Gallery


Registered: 05/29/07
Posts: 1,938
Last seen: 11 years, 10 months
Contaminated syringe?
    #7694286 - 11/29/07 10:21 AM (16 years, 2 months ago)

I recently made a bunch b+ syringes from a print, and I ended up innoculating 4 or 5 jars with one of the syringes.  All of those jars had bad contamination (all the other jars were fine- I inoculated those with tex's), so i came to the obvioyus conclusion that theres gotta be some contaminant in the b+ syringe.
How can I test to see if the other syringes are contaminated too short of innoculating more jars? I dont want to waste a bunch of cakes in case they are.
(Im assuming they all are- im thinking it was probably the print that had some kinda contam in it)
anways... any suggestions are welcome
Thanks guys

:mushroom2:


--------------------
:bouncysmoke:

I'm ok, really.


Extras: Filter Print Post Top
Invisibleimpeachme2
AmateurMycologist
 User Gallery


Registered: 05/10/07
Posts: 646
Re: Contaminated syringe? [Re: ShroomieGirl]
    #7694353 - 11/29/07 10:43 AM (16 years, 2 months ago)

There's no practical way to tell without actually trying it.


--------------------
tradelist


Extras: Filter Print Post Top
OfflineShroomieGirl
What are these god damn animals
Female User Gallery


Registered: 05/29/07
Posts: 1,938
Last seen: 11 years, 10 months
Re: Contaminated syringe? [Re: impeachme2]
    #7694885 - 11/29/07 01:37 PM (16 years, 2 months ago)

what about an lc or something? would that tell me anything?


--------------------
:bouncysmoke:

I'm ok, really.


Extras: Filter Print Post Top
Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: Contaminated syringe? [Re: ShroomieGirl]
    #7694894 - 11/29/07 01:40 PM (16 years, 2 months ago)

LC's are pretty hard to spot contams in, an lc can look ok and be contaminated.

You could try it out on agar. With the advantage that if contams do show up, transfer clean tissue and keep transfering until you have a clean sample, use that to start an LC.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
OfflineShroomieGirl
What are these god damn animals
Female User Gallery


Registered: 05/29/07
Posts: 1,938
Last seen: 11 years, 10 months
Re: Contaminated syringe? [Re: veda_sticks]
    #7694900 - 11/29/07 01:42 PM (16 years, 2 months ago)

good idea... id have to get some agars tho

but def better than just throwing away 8 syringes  :shrug:


--------------------
:bouncysmoke:

I'm ok, really.


Extras: Filter Print Post Top
Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: Contaminated syringe? [Re: ShroomieGirl]
    #7694914 - 11/29/07 01:46 PM (16 years, 2 months ago)

Yeah, look up the agar teks, i also believe there is an alternative method to agar using brf and water, though im not sure how usefull that would be in isolating as it might just give the contam lots to eat.

Check out antibiotic agar too.

All you need is 1 plate of clean tissue which could inoc lots of grain jars or LC's.

At the same time you could try your hand at isolating myc :smile:


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
OfflineShroomieGirl
What are these god damn animals
Female User Gallery


Registered: 05/29/07
Posts: 1,938
Last seen: 11 years, 10 months
Re: Contaminated syringe? [Re: veda_sticks]
    #7694920 - 11/29/07 01:48 PM (16 years, 2 months ago)

yeah... i could do all kinds of new shit w that

so many possbilities! :smile: lol


--------------------
:bouncysmoke:

I'm ok, really.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Kratom Powder for Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Media Antibiotic opioq 1,072 5 06/16/05 01:59 PM
by Roadkill
* Possible Contaminate Syring godson 1,569 5 05/16/04 09:09 PM
by ButterNut
* antibiotic agar
( 1 2 all )
5meopsyco 6,521 24 02/23/22 08:31 AM
by MysticMycologist
* Syringe Contamination TrippingBillies 1,071 2 05/22/02 08:50 AM
by PGF
* agar transfers buggers 4,514 12 11/03/07 10:44 AM
by orchidfanatic
* Does this look like contamination to you? paranoid2 1,677 9 06/17/02 09:14 PM
by durban_poison
* contamination problem abraham 1,667 3 11/07/02 10:51 AM
by abraham
* Liquid Myclium Transfer YellowSubmarine 737 2 12/10/05 08:38 PM
by in_laymans_terms

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
479 topic views. 18 members, 136 guests and 37 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.01 seconds on 14 queries.