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ShroomieGirl
What are these god damn animals



Registered: 05/29/07
Posts: 1,938
Last seen: 11 years, 10 months
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Contaminated syringe?
#7694286 - 11/29/07 10:21 AM (16 years, 2 months ago) |
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I recently made a bunch b+ syringes from a print, and I ended up innoculating 4 or 5 jars with one of the syringes. All of those jars had bad contamination (all the other jars were fine- I inoculated those with tex's), so i came to the obvioyus conclusion that theres gotta be some contaminant in the b+ syringe. How can I test to see if the other syringes are contaminated too short of innoculating more jars? I dont want to waste a bunch of cakes in case they are. (Im assuming they all are- im thinking it was probably the print that had some kinda contam in it) anways... any suggestions are welcome Thanks guys
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I'm ok, really.
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impeachme2
AmateurMycologist



Registered: 05/10/07
Posts: 646
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There's no practical way to tell without actually trying it.
-------------------- tradelist
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ShroomieGirl
What are these god damn animals



Registered: 05/29/07
Posts: 1,938
Last seen: 11 years, 10 months
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Re: Contaminated syringe? [Re: impeachme2]
#7694885 - 11/29/07 01:37 PM (16 years, 2 months ago) |
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what about an lc or something? would that tell me anything?
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I'm ok, really.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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LC's are pretty hard to spot contams in, an lc can look ok and be contaminated.
You could try it out on agar. With the advantage that if contams do show up, transfer clean tissue and keep transfering until you have a clean sample, use that to start an LC.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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ShroomieGirl
What are these god damn animals



Registered: 05/29/07
Posts: 1,938
Last seen: 11 years, 10 months
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Re: Contaminated syringe? [Re: veda_sticks]
#7694900 - 11/29/07 01:42 PM (16 years, 2 months ago) |
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good idea... id have to get some agars tho
but def better than just throwing away 8 syringes
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I'm ok, really.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Yeah, look up the agar teks, i also believe there is an alternative method to agar using brf and water, though im not sure how usefull that would be in isolating as it might just give the contam lots to eat.
Check out antibiotic agar too.
All you need is 1 plate of clean tissue which could inoc lots of grain jars or LC's.
At the same time you could try your hand at isolating myc
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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ShroomieGirl
What are these god damn animals



Registered: 05/29/07
Posts: 1,938
Last seen: 11 years, 10 months
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Re: Contaminated syringe? [Re: veda_sticks]
#7694920 - 11/29/07 01:48 PM (16 years, 2 months ago) |
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yeah... i could do all kinds of new shit w that
so many possbilities! lol
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I'm ok, really.
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