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Invisibletrscstghst
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Registered: 10/14/07
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are spores from a clone viable
    #7693650 - 11/29/07 04:49 AM (16 years, 2 months ago)

i assume the answer is yes but a little verification would be appreciated


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Why use up the forests which were centuries in the making and the mines which required ages to lay down, if we can get the equivalent of forest and mineral products in the annual growth of the hemp fields?
o Henry Ford


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Offlinepazzy
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Registered: 08/24/07
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Re: are spores from a clone viable [Re: trscstghst]
    #7693695 - 11/29/07 06:00 AM (16 years, 2 months ago)

Yes. They are viable.


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OfflineDave Bowman
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Re: are spores from a clone viable [Re: pazzy]
    #7693995 - 11/29/07 08:36 AM (16 years, 2 months ago)

Quote:

pazzy said:
Yes. They are viable.




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Invisiblemycocurious
Mike O. Kuerias
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Registered: 02/09/07
Posts: 1,265
Re: are spores from a clone viable [Re: pazzy]
    #7694051 - 11/29/07 08:57 AM (16 years, 2 months ago)

It's a really tricky question depending on what you mean by viable...

If you're asking if the spores can germinate to create new multispore cultures then yes, they are viable.

However, if you're asking if they will produce the same phenotype (observed trait) characteristics you sought to isolate through cloning then the answer is much more difficult to answer because it depends so++ many contributing factors such genetic dominance of the trait, recombination random variation/mutation, etc. The same holds true for humans in eye color. It's a safe bet that two brown-eyed people will have a brown-eyed baby but it _is_ possible that they could also have blue or green eyed babies...

Keep in mind that my understand of the peculiarities of fungal genetics is about this big... -->|.|<-- ...however, typically, the dominance relationship for phenotypes looks something like this...

genotype (inherited dna) + environment + random-genetic-variations = phenotype (an observable trait)


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Question hits close to home for me because I have a B+ culture at the moment that is producing almost golden-cinnamon colored spores instead of the typical indigo purple spores. While I'm fairly certain that a cloned isolate will produce the same characteristics, I'm unsure if the fruits grown out from the spores would continue to pass along this trait through multiple generations.


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:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
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figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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