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Invisiblemycocurious
Mike O. Kuerias
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Registered: 02/09/07
Posts: 1,265
B+ w/ Brown Spores...
    #7689722 - 11/28/07 10:05 AM (16 years, 2 months ago)

I'm familiar with most cubensis strains dropping spores in the purple to purplish-brown color range but I've recently had a set of very poor sporulators that drop a very cinnamon/golden-brown color spore and so I was wondering...

1. would this trait be preserved if I took a living clone from one of these fruits and grew it out on agar?  (i'm fairly sure the answer is yes here...)

2. if the fruits from the cloned fruits produced the same cinnamon/golden-brown spores would that trait be preserved through it's spores in future multispore inoculations?

:shrug:


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:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

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figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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OfflineWorkmanV
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Re: B+ w/ Brown Spores... [Re: mycocurious]
    #7689778 - 11/28/07 10:25 AM (16 years, 2 months ago)

If the trait is genetic, and it probably is, it should be preserved in the clone.

Assuming the above is true, the spores will produce mushrooms with like colored spores. But you may not get 100% brown spored mushrooms depending on the dominance of the mutation. In my experience, color mutations are recessive so all of the offspring should be brown spored. But since mycelium is multinucleate (usually 2 per cell) and recombination can happen within the mycelium, weird things can appear, such as chimera-like properties where some gills produce the normal spores and some the mutated spores, all on the same mushroom.

The poor spore production may be an indicator of hidden genetic problems and the culture is probably going to be less vigorous in the long run than more normal appearing strains.

Good luck!


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Invisiblemycocurious
Mike O. Kuerias
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Registered: 02/09/07
Posts: 1,265
Re: B+ w/ Brown Spores... [Re: Workman]
    #7690025 - 11/28/07 11:35 AM (16 years, 2 months ago)

Quote:

Workman said:
If the trait is genetic, and it probably is, it should be preserved in the clone.

...

The poor spore production may be an indicator of hidden genetic problems and the culture is probably going to be less vigorous in the long run than more normal appearing strains.

Good luck!




I definitely think there may be something to the second statement regarding "hidden genetic problems" because the culture is coming off one of the slowest colonizers I've seen...(72 days from inoculation until first flush) And it's only this third flush that's produced the cinnamon brown spores.  The previous two flushes where either sporeless or the normal indigo-like purple spores from poor sporulators.

One interesting environmental factor was that the compost used as a substrate had gone through an anaerobic cycle and after pasteurization gave off a distinct "rancid butter" smell - apparently butyric acid is a bi-product of anaerobic bacterial decomposition.  :shrug:

Either way, it's given me the opportunity to really delve into the realm of _real_ culture isolation and cloning.  I had originally thought this current culture was a clone grown out on agar however after seeing how it fruited and all the variations within I did a little research and learned a very valuable lesson regarding attempting to isolate from gill fragments... :foreheadslap:


--------------------
:justdontknow: Don't mistake my tone for a "matter-of-fact" attitude.  I'm just presenting what I believe to be correct, until I'm corrected...

- How Myco-Curious Prepares Coir & Compost Substrates
- How Myco-Curious Builds A Bulk Humidifier
- How Myco-Curious Builds An Automated Greenhouse
------------------------------------
figgusfiddus said:
Keep in mind that inoculating or whatever in front of a flow hood won't help your bad substrate, your bad inoculant, your bad sterile procedure, etc. etc. etc. It's not a +3 flowhood of magic, it's just a tool.


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