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Offlineresptodd
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Dry ice to pasteurize?
    #7688678 - 11/28/07 01:00 AM (13 years, 10 months ago)

I just got off the phone with a good friend who helped me out ten yrs ago when I did the Homestead Book grow. He told me the best and easiest way to pasteurize bulk subs is with dry ice. He said fill a sealable container(like a rubbermaid tub with lid) with your bulk sub. Hpoo, coir, straw, whatever and throw a bunch of dry ice in with it give it 24hrs you done. Anyone hear anything like this?


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OfflineBlargIAmDead
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Re: Dry ice to pasteurize? [Re: resptodd]
    #7688700 - 11/28/07 01:04 AM (13 years, 10 months ago)

I was about to say "No" with great vehemence  but Wikipedia changed my mind :smile:.

http://en.wikipedia.org/wiki/Food_preservation

"Grains may be preserved using carbon dioxide. A block of dry ice is placed in the bottom and the can is filled with grain. The can is then "burped" of excess gas. The carbon dioxide from the sublimation of the dry ice prevents insects, mold, and oxidation from damaging the grain. Grain stored in this way can remain edible for five years.

Nitrogen gas (N2) at concentrations of 98% or higher is also used effectively to kill insects in grain through hypoxia. However, carbon dioxide has an advantage in this respect as it kills organisms through both hypoxia and hypercarbia, requiring concentrations of only 80%, or so. This makes carbon dioxide preferable for fumigation in situations where an hermetic seal cannot be maintained."

Good idea. But I have no clue. Try it out :smile:.


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Offlineresptodd
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Re: Dry ice to pasteurize? [Re: BlargIAmDead]
    #7688714 - 11/28/07 01:07 AM (13 years, 10 months ago)

Uhhhhhh OK. So he was right?


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OfflineRogerRabbitM
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Re: Dry ice to pasteurize? [Re: resptodd]
    #7688726 - 11/28/07 01:10 AM (13 years, 10 months ago)

For bacteria and live mold, yes. I can't imagine CO2 doing much against mold spores that would germinate later during colonization.
RR


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OfflineBlargIAmDead
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Re: Dry ice to pasteurize? [Re: resptodd]
    #7688729 - 11/28/07 01:11 AM (13 years, 10 months ago)

Uhhhhhh maybe. You could test it. What it's basically saying is it will kill things that require oxygen to live by depriving them of oxygen and inundating them with CO2. So if that's how bacteria thrive then yes. I can see where it would hinder growth and possibly kill them. I wouldn't know about things like endospores. From what I read they're pretty damn hardy.


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Offlineabesh
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Re: Dry ice to pasteurize? [Re: BlargIAmDead]
    #7688734 - 11/28/07 01:12 AM (13 years, 10 months ago)

Interesting. I do remember reading a thread of preserving dried shrooms using dry ice... something along the lines of putting dry ice in a jar with shrooms, maybe separating them with a foil or something, and let the gases slowly escape, and seal the lid towards the end... or something along those lines.

But this would be a way more worthier cause to go get some dry ice.


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OfflinePr0_X
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Re: Dry ice to pasteurize? [Re: abesh]
    #7688752 - 11/28/07 01:17 AM (13 years, 10 months ago)

It'd be alot more cost effective to simply pasteurize your bulk in a hot water bath, depriving organisms of air will not kill endospores that will germinate when fruiting as RR said.


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Offlineresptodd
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Re: Dry ice to pasteurize? [Re: abesh]
    #7688758 - 11/28/07 01:18 AM (13 years, 10 months ago)

Ok, I'll try it. I'll use the dry ice on some composted Hpoo and noc' it up. I'll bring it all up again in a week or so.


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InvisibleGlacier Creek
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Re: Dry ice to pasteurize? [Re: abesh]
    #7688760 - 11/28/07 01:18 AM (13 years, 10 months ago)

So in theory one could put a small peice of dry ice in a substrate jar, that has been hydrated with h202 and a PC would not be needed. Wouldnt this kill all the live contams, and the spores as well.

Im sticking to my pc either way, but it was just a thought.


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Offlineabesh
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Re: Dry ice to pasteurize? [Re: Pr0_X]
    #7688761 - 11/28/07 01:19 AM (13 years, 10 months ago)

Ya I guess I realize that now, sorry I am stoned and type real slow... I was trying to reply after the second post. :shrug:


Edit: I give-up... you people are just too fast for me. good nite.


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Edited by abesh (11/28/07 01:21 AM)


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Offlineresptodd
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Re: Dry ice to pasteurize? [Re: abesh]
    #7688799 - 11/28/07 01:28 AM (13 years, 10 months ago)

I really think this is something to consider. I will try it on the Hpoo. Any takers on trying it on a grain or pf jar? (don't seal the jar you'll blow it up) Glacier Creek you came up with the idea willing to try it? For the benefit of the forum?


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InvisibleGlacier Creek
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Re: Dry ice to pasteurize? [Re: resptodd]
    #7688853 - 11/28/07 01:50 AM (13 years, 10 months ago)

Ill try to locate some dry ice next time I go into town. Do you think tyvek would allow enough gas exchange to move enough O2 before inoculation?


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Offlineresptodd
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Re: Dry ice to pasteurize? [Re: Glacier Creek]
    #7688977 - 11/28/07 02:58 AM (13 years, 10 months ago)

Dry ice lets off a hell of alot of gas pressure as it evaporates. I can't say tyvek would allow the gas to escape quickly enough or not. Something will give, at worst the tyvek would rupture before anything else. I can get dry ice right down the road, I'll be hitting up the Hpoo in a matter of hrs. We'll get this thing together, I'm very much looking forward to the results. hey I got an idea--put the jar in a larger container, CO2 is more dense, heavier than O2, it will fill the container leaving the jar resting nicely inside, use a very loose fitting cover. Then sterile tek the rest of the way-- maybe? Just an idea.


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Offlineresptodd
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Re: Dry ice to pasteurize? [Re: resptodd]
    #7689907 - 11/28/07 01:02 PM (13 years, 10 months ago)

OK, I have the dry ice and i'm filling a small rubbermaid tub with composted Hpoo and just dropping the dry ice on top of the Hpoo and putting on the loose fitting lid. I'll wait 24hrs and noc it up with a pf cake.


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Offlinebinarybit0101
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Re: Dry ice to pasteurize? [Re: resptodd]
    #7693113 - 11/29/07 12:57 AM (13 years, 10 months ago)

This sounds interesting, if i were to take a gallon of boiling water and pour it in a 5 gal pail with coffee grounds and a dash of crushed oyster shell and a brick of coir, cover for an hour then put it in the mono tub, would adding a pound or two of dry ice help prevent contams when i add my colonized wbs? or is that just over kill.

First grow, much luv for the community here:rasta:. will post grow log soon.


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InvisibleGlacier Creek
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Re: Dry ice to pasteurize? [Re: binarybit0101]
    #7693498 - 11/29/07 03:45 AM (13 years, 10 months ago)

A polyfill jar lid would work though.

I know h202 will kill mold spores but will it kill grain endospores? I am just thinking this could be a way someone could get a couple grows off before they buy a pressure cooker, but not have to use BRF.


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Edited by Glacier Creek (11/29/07 03:48 AM)


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Offlineresptodd
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Re: Dry ice to pasteurize? [Re: Glacier Creek]
    #7694848 - 11/29/07 03:27 PM (13 years, 10 months ago)

Well to bring this back to life. I just finished the "dry ice pasteurization" of a composted Hpoo pan. I put the Hpoo pan in a larger rubbermaid contianer left the top off the Hpoo pan fill the rubbermaid with about 5 lbs(overkill) of dry ice an lidded the large container. Took it out this morning, cooked maybe 30hrs, and noc'd it up with a pf cake. Let ya' all know in a week or so.


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OfflineFraggin
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Re: Dry ice to pasteurize? [Re: resptodd]
    #7694922 - 11/29/07 03:48 PM (13 years, 10 months ago)

Quote:

resptodd said:
Well to bring this back to life. I just finished the "dry ice pasteurization" of a composted Hpoo pan. I put the Hpoo pan in a larger rubbermaid contianer left the top off the Hpoo pan fill the rubbermaid with about 5 lbs(overkill) of dry ice an lidded the large container. Took it out this morning, cooked maybe 30hrs, and noc'd it up with a pf cake. Let ya' all know in a week or so.




Not to be a kill joy, but hpoo is not a good substrate to do this experiment with. There are too many positive benefits of hpoo that will render your experiment a false positive for success. I only say this because I have experimented with using un-pastuerized hpoo as a bulk substrate and have been succesful on more than two occasions.


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Invisiblenovicemycology
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Re: Dry ice to pasteurize? [Re: Fraggin]
    #7695603 - 11/29/07 06:45 PM (13 years, 10 months ago)

Quote:

Fraggin said:
Quote:

resptodd said:
Well to bring this back to life. I just finished the "dry ice pasteurization" of a composted Hpoo pan. I put the Hpoo pan in a larger rubbermaid contianer left the top off the Hpoo pan fill the rubbermaid with about 5 lbs(overkill) of dry ice an lidded the large container. Took it out this morning, cooked maybe 30hrs, and noc'd it up with a pf cake. Let ya' all know in a week or so.




Not to be a kill joy, but hpoo is not a good substrate to do this experiment with. There are too many positive benefits of hpoo that will render your experiment a false positive for success. I only say this because I have experimented with using un-pastuerized hpoo as a bulk substrate and have been succesful on more than two occasions.






Great point. It's going to take a handfull of back-to-back successful tries to make me a believer. Good luck, I hope it works. I'll be keeping an eye on this idea.


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Offlineacommunistspy
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Re: Dry ice to pasteurize? *DELETED* [Re: novicemycology]
    #7695643 - 11/29/07 06:55 PM (13 years, 10 months ago)

Post deleted by acommunistspy

Reason for deletion: this never happened



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