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Invisibletahoe
Noob Slayer
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Limited Gene Pool- No Diversity???
    #7687270 - 11/27/07 05:46 PM (16 years, 2 months ago)

Some of you might have seen pics of the outdoor grow that I had going and I have a question about it.
The patch was made from a single mushroom-clone. I cloned it to agar then to a lc and then to seed and finally to wood chips.

Now the patch Produced a good first flush although not across the entire patch. Actually only about 20% of it produced mushrooms but it did resemble a wild outdoor patch so that isn't my big concern.

Now there are just a few stragglers left and I was hoping to see a second flush come out of it but nothing. I am wondering if since it was cloned and I am assuming that all the mycelium is genetically the same will the patch only fruit when conditions are right for that specific genetic makeup?

I am assuming that this is why it is better to have multi spore inoculations and this is what happens when one over transfers and isolated sectors of mycelium.

Am I the right track here?

Also the mycelium is very vigorous in sections and it has grown over the tops of the wood chips and I can visually see rhizomorphs on the top of the patch. It almost resembles overlay on a casing layer. These areas never fruited.

What do you folk think?


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibleimpeachme2
AmateurMycologist
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Registered: 05/10/07
Posts: 646
Re: Limited Gene Pool- No Diversity??? [Re: tahoe]
    #7687986 - 11/27/07 08:05 PM (16 years, 2 months ago)

Practically all variations of mycelium from a given parent will fruit under the same range of conditions, like any other organism. It is very likely something else is hindering another flush, if not time itself.


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tradelist


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Invisibletahoe
Noob Slayer
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Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Limited Gene Pool- No Diversity??? [Re: impeachme2]
    #7692434 - 11/28/07 08:06 PM (16 years, 2 months ago)

this isnt a culture from spores, it was a clone. I thought this would limit the variation


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Invisibleimpeachme2
AmateurMycologist
 User Gallery


Registered: 05/10/07
Posts: 646
Re: Limited Gene Pool- No Diversity??? [Re: tahoe]
    #7692585 - 11/28/07 08:41 PM (16 years, 2 months ago)

I know. I'm saying unless what you cloned from was extremely abnormal, it should fruit under the same conditions as a multi-spore outdoor grow.


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tradelist


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