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Offlinekatfish
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Registered: 07/22/06
Posts: 592
Loc: Louisiana
Last seen: 15 years, 11 months
making syringes without a glovebox
    #7686460 - 11/27/07 03:26 PM (16 years, 2 months ago)

Does anyone have success when making syringes without a glovebox?

I do want to make a glovebox one day but I am too busy at the moment.
I made a successful PESA syringe off of a print that I made from my first grow but I figured i just got lucky.

I do it in the bathroom, I spray the hell out of the countertops and air with disinfectant and close the door. Then after a few minutes I enter with isopropyl alcohol cleaned hands up to my elbows and have the print and a syringe with sterile water in it. I hold the print over a sterile plastic cup and spray the spores off the foil print into the glass with water from the syringe. I find this is the easiest way to get the spores off there without using a loop. I nocced up a jar of WBS and it is bight white mycelium growing nicely!

kF


--------------------
08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!


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OfflineRogerRabbitM
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Re: making syringes without a glovebox [Re: katfish]
    #7686485 - 11/27/07 03:32 PM (16 years, 2 months ago)

Stick your gloved hands and jars/syringes deep into a clear trash bag, for an instant cheap glovebox.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Offlinekatfish
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Re: making syringes without a glovebox [Re: RogerRabbit]
    #7686512 - 11/27/07 03:40 PM (16 years, 2 months ago)

hmm. great advice as usual!

why didn't i think of that, that is why you are the MAN!  :headbanger:  :rockon:  :headbang:


--------------------
08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!


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Offlinenavyseals101
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Re: making syringes without a glovebox [Re: RogerRabbit]
    #7686518 - 11/27/07 03:41 PM (16 years, 2 months ago)

^ a clear trash bag?

my trashbags are usually yellow or white, where do i find the clear ones at lol


--------------------
"Ten thousand flowers in spring
the moon in autumn,
a cool breeze in summer,
snow in winter.
If your mind isn't clouded by unnecessary things,
this is the best season of your life."
            -wumen


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Offlinerobanero
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Re: making syringes without a glovebox [Re: katfish]
    #7686530 - 11/27/07 03:44 PM (16 years, 2 months ago)

I do want to make a glovebox one day but I am too busy at the moment.

It's very quick and easy to make a still air box like in RR video. Buy a large enough clear tub and using a six inch hole saw(the type one puts on a drill) cut two holes in the side. The objective is to stop the movement of air and contams. Very easy and should only take about 5 Min's.

The way you do yours is fine,as long as you spray the shit out of the air. I would spay your cloths also to eliminate any contams on them.


--------------------

310 open cap Hawaiians grown a 1 pint WBSF cake.

SPAWN RATIO CALCULATOR


My Little Hawaiians

Spawn Bag Tek


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Invisiblelegallyhomeless
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Re: making syringes without a glovebox [Re: navyseals101]
    #7686546 - 11/27/07 03:47 PM (16 years, 2 months ago)

I made my first syringes in open air after spraying lysol. Worked fine. Be CLEAN


--------------------
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FULLY AUTOMATIC!! 12-Pot Multi Grow Hydroponic System for Trade.


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Invisiblemusher_420
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Registered: 08/01/05
Posts: 2,691
Re: making syringes without a glovebox [Re: katfish]
    #7686692 - 11/27/07 04:23 PM (16 years, 2 months ago)

You might wanna check this out for making future syringes. I found this method works really good. Just be quick as possible when transferring the cap in and out of the jar and you should have a really good success rate.

http://www.shroomery.org/forums/showflat.php/Number/5276177#Post5276177

Searching all of Agar's posts will yield multitudes of well written and very visual posts.


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Invisiblegoldieman
On some other shit!


Registered: 08/15/07
Posts: 296
Loc: midwest
Re: making syringes without a glovebox [Re: musher_420]
    #7687042 - 11/27/07 05:14 PM (16 years, 2 months ago)

I USUALLY TURN THE STOVE ON, PULL OUT A RACK, COVER IT WITH ALUMINUM FOIL AND WORK IN THE WARM UPDRAFT. CONSTANTLY SWABING WITH ALCOHOL AND LOTS OF HAND SANITIZER.


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Offlinekatfish
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Re: making syringes without a glovebox [Re: goldieman]
    #7689064 - 11/28/07 01:35 AM (16 years, 2 months ago)

i am gonna not worry so much, i was testing my syringes on agar plates but those I found out were getting contamed, not from the syringes but from the agar getting air contams when i opened the dish without a glovebox.

kF


--------------------
08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!


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OfflineBlargIAmDead
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Re: making syringes without a glovebox [Re: katfish]
    #7689077 - 11/28/07 01:42 AM (16 years, 2 months ago)

goldie...wtf? Seriously, hope you're joking.


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Offlinekatfish
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Re: making syringes without a glovebox [Re: BlargIAmDead]
    #7689091 - 11/28/07 02:02 AM (16 years, 2 months ago)

goldie?


--------------------
08/2/07 Inoculated mycobag with Brazilian, 3cc
08/08/07 First mycelium show
09/03/07 Brazilian still colonizing, Three Mycobags inoculated with Koi Somui
10/11/07 2 Pint PESA 100% colonized. 1 pint Amazon 90% colonized
10/12/07 Storage bin perite FC constructed
10/28/07 Havested my First ever flush, of PESA!


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OfflineRogerRabbitM
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Re: making syringes without a glovebox [Re: katfish]
    #7689123 - 11/28/07 02:34 AM (16 years, 2 months ago)

The so-called oven tek was discredited years ago. It increases contamination risk by increasing drafts. That warm draft has to come from somewhere, and where it comes from is full of mold and bacteria.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


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Invisibleohmatic
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Loc: europe
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Re: making syringes without a glovebox [Re: RogerRabbit]
    #7689132 - 11/28/07 02:44 AM (16 years, 2 months ago)

i fixed my very first spore syringe ever without anything in open air,
i worked fast but not hastily and made sure everything was clean.

worked good without problems.


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Offlineveda_sticks
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Re: making syringes without a glovebox [Re: ohmatic]
    #7689291 - 11/28/07 06:30 AM (16 years, 2 months ago)

I used the oven tek a few times, i had 1 succesfull run of about 4 jars, the rest i had about a 90% contamination rate.

I had better success in open air.

And now i use a glovebox, which took 5 minutes to make and cost me nothing as i had a big clear plastic container just lying around.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


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OfflinePr0_X
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Registered: 01/18/06
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Re: making syringes without a glovebox [Re: veda_sticks]
    #7689434 - 11/28/07 08:02 AM (16 years, 2 months ago)



--------------------
It's okay to hurt my feelings
cause you know, they're so numb anyway.
but I guess it's what I get
for being to fuckin stupid to stay away - Jake

- Support the FSR at www.fsrcanada.com and www.fsre.nl


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InvisibleBridgeburner
Not spiritual at all.
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Re: making syringes without a glovebox [Re: legallyhomeless]
    #7689505 - 11/28/07 08:40 AM (16 years, 2 months ago)

Quote:

legallyhomeless said:
I made my first syringes in open air after spraying lysol. Worked fine. Be CLEAN




--------------------


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OfflineanarchOi
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Re: making syringes without a glovebox [Re: Bridgeburner]
    #7689536 - 11/28/07 08:54 AM (16 years, 2 months ago)

if you use a trashbag, make sure you don't do the thing where you fill it with air to get it unfolded

otherwise you might as well do it in open air :-P


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Invisibletahoe
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Re: making syringes without a glovebox [Re: anarchOi]
    #7692713 - 11/28/07 09:05 PM (16 years, 2 months ago)

I used to have good luck by washing out a shot glass with alcohol and making sure it was completely dry. scraping some spores into the glass and squirting 1/8th cc of the syringe in there and sucking it back up. The faster the better.
Now I just use sandwich bags, sort of like what double r said but on the small scale


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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