|
 Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
 usr
Interested.
Registered: 09/16/07
Posts: 164
Loc: United States
Last seen: 9 months, 21 days
|
 Absolutely no colonization after a week. 
#7677973 - 11/25/07 01:33 PM (16 years, 4 months ago) |
|
|
I'm not sure what's going on. I know it takes a while to show signs of growth sometimes, but none of the jars are showing anything at all. I inoculated 10 jars with Golden Teacher 10 days ago.
I used the same procedure I used the last time, my first grow, and had around 20 cakes all do very well. Those jars showed signs of colonization within 5-7 days.
Any idea what the problem could be? I'm incubating around 86, the same way I did the last time.
|
johnnyfacker
Stranger
Registered: 10/05/07
Posts: 78
Last seen: 14 years, 11 months
|
Re: Absolutely no colonization after a week. [Re: usr]
#7678012 - 11/25/07 01:44 PM (16 years, 4 months ago) |
|
|
Wait a few more days, only time will tell. However it is possible you got a crappy syringe(if everything was done exactly the same),also did you let the substrate cool enough b/f inoculation?Guess you'll find out in a couple days, but 12 days or so seems like a long time to me too!! Good luck.
|
 Premedman1
Assistant to the insistent
Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
|
Re: Absolutely no colonization after a week. [Re: johnnyfacker]
#7678018 - 11/25/07 01:46 PM (16 years, 4 months ago) |
|
|
Your incubation temps are a bit too warm. Bring them down to around 80 if possible.
--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
|
Mojo
Stranger
Registered: 07/12/07
Posts: 1,677
|
Re: Absolutely no colonization after a week. [Re: usr]
#7678024 - 11/25/07 01:48 PM (16 years, 4 months ago) |
|
|
Could your temps be creeping up on you? Maybe you should lower your temps a bit.
|
Mojo
Stranger
Registered: 07/12/07
Posts: 1,677
|
Re: Absolutely no colonization after a week. [Re: Premedman1]
#7678026 - 11/25/07 01:48 PM (16 years, 4 months ago) |
|
|
Damn you beat me to it. 
|
acommunistspy
the fun nazi,NOT to beconfused withthe fun-nazi
Registered: 08/13/07
Posts: 657
Loc: the garden state
Last seen: 14 years, 30 days
|
Re: Absolutely no colonization after a week. *DELETED* [Re: Mojo]
#7678260 - 11/25/07 02:33 PM (16 years, 4 months ago) |
|
|
Post deleted by acommunistspyReason for deletion: this never happened
--------------------
|
usr
Interested.
Registered: 09/16/07
Posts: 164
Loc: United States
Last seen: 9 months, 21 days
|
Re: Absolutely no colonization after a week. [Re: acommunistspy]
#7679131 - 11/25/07 05:52 PM (16 years, 4 months ago) |
|
|
I'll lower my temps, but last time I had jars colonizing at around 90 (too hot, I know).
I let the jars cool for over 24 hours before inoculating.
Got them from Hawks Eye.
|
Deception
HopefullyTripping Soon
Registered: 09/12/06
Posts: 88
Loc: Somewhere Over the Rainbo...
Last seen: 13 years, 4 months
|
Re: Absolutely no colonization after a week. [Re: usr]
#7679217 - 11/25/07 06:21 PM (16 years, 4 months ago) |
|
|
having the same problem. . noticed that my syringes didn't look like they had anything in them. . just figured that i wouldn't be able to see the spores but then i see pictures online of syringes with black dots very visible so i think i got bad ones too. . three failed attempts in a row. . no contams just no growth. good luck to you i'm going to get new syringes and try a lc next time
--------------------
If at first you don't succeed..
Hit it till your knuckles bleed..
|
acommunistspy
the fun nazi,NOT to beconfused withthe fun-nazi
Registered: 08/13/07
Posts: 657
Loc: the garden state
Last seen: 14 years, 30 days
|
Re: Absolutely no colonization after a week. *DELETED* [Re: Deception]
#7679569 - 11/25/07 07:46 PM (16 years, 4 months ago) |
|
|
Post deleted by acommunistspyReason for deletion: this never happened
--------------------
|
Premedman1
Assistant to the insistent
Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
|
Re: Absolutely no colonization after a week. [Re: acommunistspy]
#7679606 - 11/25/07 07:55 PM (16 years, 4 months ago) |
|
|
Quote:
acommunistspy said:
yea fuck what other people say about spores being microscopic and shit.
??? 
--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
|
c0_hush
Stranger
Registered: 07/16/06
Posts: 417
Last seen: 12 years, 7 months
|
Re: Absolutely no colonization after a week. [Re: acommunistspy]
#7679629 - 11/25/07 07:59 PM (16 years, 4 months ago) |
|
|
I got 2 syringes from Spores 101 that looked clear, even close up but I remembered what I read on here and inoculated 8 half pint jars with A strain and the other 4 with Cambodian. It's day 14 now and they are 85% colonized. I lost one of the Cambodian to green mold. I had also ordered syringes from The Hawk's Eye and recently used Hawaiian to inoculate 16 quarter pint jars on Nov 17 and I'm seeing around 10% growth on those as well. T.H.E. syringes were dark vs Spores 101 clear but they both seem to work.
|
acommunistspy
the fun nazi,NOT to beconfused withthe fun-nazi
Registered: 08/13/07
Posts: 657
Loc: the garden state
Last seen: 14 years, 30 days
|
Re: Absolutely no colonization after a week. *DELETED* [Re: Premedman1]
#7679645 - 11/25/07 08:01 PM (16 years, 4 months ago) |
|
|
Post deleted by acommunistspyReason for deletion: this never happened
--------------------
Edited by acommunistspy (11/25/07 08:03 PM)
|
c0_hush
Stranger
Registered: 07/16/06
Posts: 417
Last seen: 12 years, 7 months
|
Re: Absolutely no colonization after a week. [Re: acommunistspy]
#7679651 - 11/25/07 08:03 PM (16 years, 4 months ago) |
|
|
I personally doubt a 0.5ml solution is enough to throw water ratio out of whack but then again, I'm new to this..
|
Premedman1
Assistant to the insistent
Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
|
Re: Absolutely no colonization after a week. [Re: acommunistspy]
#7679663 - 11/25/07 08:06 PM (16 years, 4 months ago) |
|
|
Quote:
acommunistspy said:
if it's clear, that means there's not much there (IMHO)
Can't argue with that logic since spores aren't really microscopic.  
--------------------
Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
|
tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
|
Re: Absolutely no colonization after a week. [Re: usr]
#7680051 - 11/25/07 10:08 PM (16 years, 4 months ago) |
|
|
Quote:
usr said:
I'm not sure what's going on. I know it takes a while to show signs of growth sometimes, but none of the jars are showing anything at all. I inoculated 10 jars with Golden Teacher 10 days ago.
I used the same procedure I used the last time, my first grow, and had around 20 cakes all do very well. Those jars showed signs of colonization within 5-7 days.
It is a little warm but let me guess. Your first grow was not golden teachers? Myabe eq's or b+?
Gt's tend to germinate late, up to three weeks. as long as you do not see some slime or mold growing in there then you wait.
--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987
Teh=The
I need to proofread
|
Enothe
Proliferater
Registered: 11/11/07
Posts: 149
Last seen: 10 years, 3 months
|
Re: Absolutely no colonization after a week. [Re: tahoe]
#7680328 - 11/26/07 12:48 AM (16 years, 4 months ago) |
|
|
my first syringes i couldn't see anything in them at all.
yet they germinated in 3 days and colonized very fast!
my recent syringes had visible spores, yet took 2 weeks to barely germinate, i think they were very poor =(
i doubt its the strain, all my GTs took 3 days so far. its probly just a shitty syringe=/
--------------------
"Never Knows Best"
|
|
Threaded
Previous
Index
Next
Bulk Cannabis Seeds 
All-In-One Bags That Don't Suck 
Bulk Substrate 
Liquid Cultures 
Buy Kratom Extract 
Golden Teacher Liquid Culture For Sale 
Bridgetown Botanicals
Edit 
Reply 
Absolut
psyphon
1 2 all )