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OfflinePr0_X
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Need help with agar
    #7672866 - 11/23/07 10:58 PM (16 years, 2 months ago)

I'm new to the whole agar thing, I've got the jist of it down...

I inoculated a few grain jars with some Texas spores from 2002, got a little bit of growth and decided to isolated it out on agar....

Here's the growth in the jar.




It seems to all be even growth right now (Its a really bad pic) my question is if i should begin transfering to new petri's?

thanks in advance.


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OfflineRogerRabbitM
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Re: Need help with agar [Re: Pr0_X]
    #7672918 - 11/23/07 11:21 PM (16 years, 2 months ago)

Go around the leading edge of the growth, and transfer small pieces all the way around. Take pieces about the size of a grain of rice. Repeat in a few days with the new dishes. By the second transfer, you should see clear sectoring. Transfer each sector to new dishes.
RR


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OfflineNo_Life_G33k
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Registered: 03/08/05
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Re: Need help with agar [Re: RogerRabbit]
    #7678608 - 11/25/07 03:44 PM (16 years, 2 months ago)

Roger- you mentioned that you go through approx. 40 dishes when starting from spores, and you end up with 10-15 isolates.

Are the isolate dishes included in the 40???

What do you do to get to your isolates??? I never found the conclusion of your 'isolates' thread.

Mucho Thanx


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