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Offlineresptodd
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D5W for LC
    #7671976 - 11/23/07 07:01 PM (16 years, 2 months ago)

Anyone in the medical field? I have a 1000cc bag of D5W(5% dextrose in water) would this be a good LC? I can always add(sterile) water to bring the % to 4%. But is dextrose a good sugar source?


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Offlineboingkster
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Re: D5W for LC [Re: resptodd]
    #7672012 - 11/23/07 07:06 PM (16 years, 2 months ago)

Dextrose is an excellent source of sugar, yes - its a substance metabolised quickly and easily by a host of organisms: yeast, mycelium... and us.

I'm thinking perhaps a stronger solution may be more beneficial, but medical grade D5W should be nice - try it out and see anyway. I just used a [non-measured] dollop of honey in a pint jar of water.
:rolleyes:

Cheers - boingkster


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OfflineNibin
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Re: D5W for LC [Re: boingkster]
    #7672108 - 11/23/07 07:20 PM (16 years, 2 months ago)

Dextrose is used to make a kind of agar also.

Oh and btw Dextrose = glucose, or more specifically D-glucose the biologically useable isomer of glucose


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Offlineresptodd
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Re: D5W for LC [Re: Nibin]
    #7672235 - 11/23/07 07:41 PM (16 years, 2 months ago)

So, what info I've gathered, I could just straight up use the D5W? If that's the case, could I introduce a spore syringe directly into the IV bag? Or gas xchange problem?


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Edited by resptodd (11/23/07 07:42 PM)


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OfflineNibin
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Re: D5W for LC [Re: resptodd]
    #7672293 - 11/23/07 07:51 PM (16 years, 2 months ago)

the only doubt I have is that if white sugar won't work in an lc, will a glucose solution.

I know Karo is glucose and fructose. But will glucose on it's own work?


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Offlineresptodd
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Re: D5W for LC [Re: Nibin]
    #7672303 - 11/23/07 07:52 PM (16 years, 2 months ago)

Is glucose dextrose? The IV bag is a 5% dextrose solution.


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Offlineboingkster
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Re: D5W for LC [Re: resptodd]
    #7672333 - 11/23/07 07:57 PM (16 years, 2 months ago)

Like I said, try it. Or even better do a side-by-side comparo...just inoculate a sterilised jar of the D5W and a jar of regular honey water [@ a 5% by volume mix] and see which one goes better. If the D5W fails, well, you've found something out haven't you? And you've still got a viable LC because of the honey water jar.

Win-win!


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OfflineNibin
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Re: D5W for LC [Re: resptodd]
    #7672354 - 11/23/07 08:02 PM (16 years, 2 months ago)

Simple answer, yes they are the same.


Complex answer, there are two possible isomers for the glucose molecule, D-Glucose and L-Glucose. D-glucose is the one produced by all living things and is the one all living things can consume. L-Glucose while it can be synthesized is no good to us. D-Glucose is also known as Dextrose (as in Dextrorotary Glucose) in the food industry. For all intents and purposes when we speak of glucose we mean D-Glucose which is the same as Dextrose


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Offlineacommunistspy
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Re: D5W for LC *DELETED* [Re: Nibin]
    #7672383 - 11/23/07 08:07 PM (16 years, 2 months ago)

Post deleted by acommunistspy

Reason for deletion: this never happened



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OfflineNibin
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Re: D5W for LC [Re: acommunistspy]
    #7672398 - 11/23/07 08:10 PM (16 years, 2 months ago)

Edit, was wrong here


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Edited by Nibin (11/24/07 03:28 AM)


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Offlineresptodd
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Re: D5W for LC [Re: Nibin]
    #7672404 - 11/23/07 08:12 PM (16 years, 2 months ago)

So, is the D5W solution D-glucose? Please bring it down to me. This is a medically used solution, for intra-venous use. Again, bring it down, can this work? or not? I would love to think I can take this solution fill some sterile jars, inoc. it up, and have a viable LC, but I'd rather not waste my time if it is a no go.


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Offlinetotal
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Re: D5W for LC [Re: resptodd]
    #7672515 - 11/23/07 08:52 PM (16 years, 2 months ago)

HERE is a link for you guys...


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OfflineQDP843
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Re: D5W for LC [Re: total]
    #7672567 - 11/23/07 09:10 PM (16 years, 2 months ago)

and where might u find these?


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Offlineresptodd
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Re: D5W for LC [Re: acommunistspy]
    #7672672 - 11/23/07 09:44 PM (16 years, 2 months ago)

I'm just gonna' take a whole GD spore syringe and knock the damn thing up. If it fails screw it.


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Offlineresptodd
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Re: D5W for LC [Re: resptodd]
    #7672680 - 11/23/07 09:46 PM (16 years, 2 months ago)

So which would be better 5% dextrose and water or 5% dextrose and 0.9% saline(normal saline, human compatible)?


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Offlineacommunistspy
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Re: D5W for LC *DELETED* [Re: resptodd]
    #7672825 - 11/23/07 10:40 PM (16 years, 2 months ago)

Post deleted by acommunistspy

Reason for deletion: this never happened



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OfflineNickio
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Re: D5W for LC [Re: acommunistspy]
    #7673161 - 11/24/07 01:08 AM (16 years, 2 months ago)

More spores can slow down innoc time. I'd toss in .5 cc and if it doesnt work, go back to the drawing board because thats enough spores for sure. Give at least 5-7 days before calling it a failure.


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InvisibleOptx
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Re: D5W for LC [Re: Nickio]
    #7673372 - 11/24/07 02:21 AM (16 years, 2 months ago)

Quote:

Nickio said:
More spores can slow down innoc time. I'd toss in .5 cc and if it doesnt work, go back to the drawing board because thats enough spores for sure. Give at least 5-7 days before calling it a failure.




what.
more spores will not slow down inoculation time in an LC...that is just dead wrong. and putting .5cc into a 1000cc bag is like trying to kill somebody with a rubberband gun.


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OfflineNibin
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Re: D5W for LC [Re: resptodd]
    #7673483 - 11/24/07 03:28 AM (16 years, 2 months ago)

I'm trying to find out on another post why plain sugar won't work in a LC.

I suspect it is because there is no salt available which would mean you might have problems as the drip is D5W which contains no salts.

Probably 2/3D & 1/3S drip would be better


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OfflineBleep75
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Re: D5W for LC [Re: resptodd]
    #28093384 - 12/11/22 01:37 PM (1 year, 1 month ago)

Did the D5 work for LC?  And was it just D5 in water or was it in D5 in .9% saline.  ?


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InvisibleShrimps
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Re: D5W for LC [Re: Bleep75]
    #28093387 - 12/11/22 01:38 PM (1 year, 1 month ago)

15 years, 21 days ago was the last post. I wouldn't give much hope in a reply ^^ :hug:


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OnlineScrewup
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Re: D5W for LC [Re: Bleep75] * 1
    #28093423 - 12/11/22 02:10 PM (1 year, 1 month ago)

Quote:

Bleep75 said:
Did the D5 work for LC?  And was it just D5 in water or was it in D5 in .9% saline.  ?



Fifteen
years
ago
You think they gonna respond?


Edited by Screwup (12/11/22 02:10 PM)


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