|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
resptodd
I reject yourreality andsubstitute myown



Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
|
D5W for LC
#7671976 - 11/23/07 07:01 PM (16 years, 2 months ago) |
|
|
Anyone in the medical field? I have a 1000cc bag of D5W(5% dextrose in water) would this be a good LC? I can always add(sterile) water to bring the % to 4%. But is dextrose a good sugar source?
-------------------- Damn! I'm having fun! Just keep the GD monkeys away.
|
boingkster
CuriousCultivator


Registered: 09/28/07
Posts: 109
Last seen: 14 years, 1 month
|
Re: D5W for LC [Re: resptodd]
#7672012 - 11/23/07 07:06 PM (16 years, 2 months ago) |
|
|
Dextrose is an excellent source of sugar, yes - its a substance metabolised quickly and easily by a host of organisms: yeast, mycelium... and us.
I'm thinking perhaps a stronger solution may be more beneficial, but medical grade D5W should be nice - try it out and see anyway. I just used a [non-measured] dollop of honey in a pint jar of water.

Cheers - boingkster
-------------------- A Question Never Asked is an Answer Never Given [Having said that, use the Search button! Teks too!]
|
Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
|
|
Dextrose is used to make a kind of agar also.
Oh and btw Dextrose = glucose, or more specifically D-glucose the biologically useable isomer of glucose
-------------------- Newcomers guide-----> For all things shroomy
|
resptodd
I reject yourreality andsubstitute myown



Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
|
Re: D5W for LC [Re: Nibin]
#7672235 - 11/23/07 07:41 PM (16 years, 2 months ago) |
|
|
So, what info I've gathered, I could just straight up use the D5W? If that's the case, could I introduce a spore syringe directly into the IV bag? Or gas xchange problem?
-------------------- Damn! I'm having fun! Just keep the GD monkeys away.
Edited by resptodd (11/23/07 07:42 PM)
|
Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
|
Re: D5W for LC [Re: resptodd]
#7672293 - 11/23/07 07:51 PM (16 years, 2 months ago) |
|
|
the only doubt I have is that if white sugar won't work in an lc, will a glucose solution.
I know Karo is glucose and fructose. But will glucose on it's own work?
-------------------- Newcomers guide-----> For all things shroomy
|
resptodd
I reject yourreality andsubstitute myown



Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
|
Re: D5W for LC [Re: Nibin]
#7672303 - 11/23/07 07:52 PM (16 years, 2 months ago) |
|
|
Is glucose dextrose? The IV bag is a 5% dextrose solution.
-------------------- Damn! I'm having fun! Just keep the GD monkeys away.
|
boingkster
CuriousCultivator


Registered: 09/28/07
Posts: 109
Last seen: 14 years, 1 month
|
Re: D5W for LC [Re: resptodd]
#7672333 - 11/23/07 07:57 PM (16 years, 2 months ago) |
|
|
Like I said, try it. Or even better do a side-by-side comparo...just inoculate a sterilised jar of the D5W and a jar of regular honey water [@ a 5% by volume mix] and see which one goes better. If the D5W fails, well, you've found something out haven't you? And you've still got a viable LC because of the honey water jar.
Win-win!
-------------------- A Question Never Asked is an Answer Never Given [Having said that, use the Search button! Teks too!]
|
Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
|
Re: D5W for LC [Re: resptodd]
#7672354 - 11/23/07 08:02 PM (16 years, 2 months ago) |
|
|
Simple answer, yes they are the same.
Complex answer, there are two possible isomers for the glucose molecule, D-Glucose and L-Glucose. D-glucose is the one produced by all living things and is the one all living things can consume. L-Glucose while it can be synthesized is no good to us. D-Glucose is also known as Dextrose (as in Dextrorotary Glucose) in the food industry. For all intents and purposes when we speak of glucose we mean D-Glucose which is the same as Dextrose
-------------------- Newcomers guide-----> For all things shroomy
|
acommunistspy
the fun nazi,NOT to beconfused withthe fun-nazi



Registered: 08/13/07
Posts: 657
Loc: the garden state
Last seen: 13 years, 10 months
|
Re: D5W for LC *DELETED* [Re: Nibin]
#7672383 - 11/23/07 08:07 PM (16 years, 2 months ago) |
|
|
Post deleted by acommunistspyReason for deletion: this never happened
--------------------
|
Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
|
|
Edit, was wrong here
-------------------- Newcomers guide-----> For all things shroomy
Edited by Nibin (11/24/07 03:28 AM)
|
resptodd
I reject yourreality andsubstitute myown



Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
|
Re: D5W for LC [Re: Nibin]
#7672404 - 11/23/07 08:12 PM (16 years, 2 months ago) |
|
|
So, is the D5W solution D-glucose? Please bring it down to me. This is a medically used solution, for intra-venous use. Again, bring it down, can this work? or not? I would love to think I can take this solution fill some sterile jars, inoc. it up, and have a viable LC, but I'd rather not waste my time if it is a no go.
-------------------- Damn! I'm having fun! Just keep the GD monkeys away.
|
total
Post Office Tyvek Advocator




Registered: 10/03/04
Posts: 11,405
Last seen: 6 months, 23 days
|
Re: D5W for LC [Re: resptodd]
#7672515 - 11/23/07 08:52 PM (16 years, 2 months ago) |
|
|
HERE is a link for you guys...
|
QDP843
Entrepreneur


Registered: 03/05/07
Posts: 295
Loc: SOUTHERN
Last seen: 15 years, 3 months
|
Re: D5W for LC [Re: total]
#7672567 - 11/23/07 09:10 PM (16 years, 2 months ago) |
|
|
and where might u find these?
|
resptodd
I reject yourreality andsubstitute myown



Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
|
|
I'm just gonna' take a whole GD spore syringe and knock the damn thing up. If it fails screw it.
-------------------- Damn! I'm having fun! Just keep the GD monkeys away.
|
resptodd
I reject yourreality andsubstitute myown



Registered: 10/16/07
Posts: 674
Loc: Michigan
Last seen: 1 year, 10 months
|
Re: D5W for LC [Re: resptodd]
#7672680 - 11/23/07 09:46 PM (16 years, 2 months ago) |
|
|
So which would be better 5% dextrose and water or 5% dextrose and 0.9% saline(normal saline, human compatible)?
-------------------- Damn! I'm having fun! Just keep the GD monkeys away.
|
acommunistspy
the fun nazi,NOT to beconfused withthe fun-nazi



Registered: 08/13/07
Posts: 657
Loc: the garden state
Last seen: 13 years, 10 months
|
Re: D5W for LC *DELETED* [Re: resptodd]
#7672825 - 11/23/07 10:40 PM (16 years, 2 months ago) |
|
|
Post deleted by acommunistspyReason for deletion: this never happened
--------------------
|
Nickio
Authority


Registered: 03/14/07
Posts: 321
Last seen: 13 years, 5 months
|
|
More spores can slow down innoc time. I'd toss in .5 cc and if it doesnt work, go back to the drawing board because thats enough spores for sure. Give at least 5-7 days before calling it a failure.
--------------------
|
Optx
PronouncedAwp-Tiks


Registered: 03/28/05
Posts: 977
|
Re: D5W for LC [Re: Nickio]
#7673372 - 11/24/07 02:21 AM (16 years, 2 months ago) |
|
|
Quote:
Nickio said: More spores can slow down innoc time. I'd toss in .5 cc and if it doesnt work, go back to the drawing board because thats enough spores for sure. Give at least 5-7 days before calling it a failure.
what. more spores will not slow down inoculation time in an LC...that is just dead wrong. and putting .5cc into a 1000cc bag is like trying to kill somebody with a rubberband gun.
-------------------- please do not take everything i say here so personally. welcome to teh internets!
|
Nibin
Getting there



Registered: 11/29/05
Posts: 4,480
Last seen: 10 years, 8 months
|
Re: D5W for LC [Re: resptodd]
#7673483 - 11/24/07 03:28 AM (16 years, 2 months ago) |
|
|
I'm trying to find out on another post why plain sugar won't work in a LC.
I suspect it is because there is no salt available which would mean you might have problems as the drip is D5W which contains no salts.
Probably 2/3D & 1/3S drip would be better
-------------------- Newcomers guide-----> For all things shroomy
|
Bleep75
Stranger
Registered: 11/24/22
Posts: 1
Last seen: 6 months, 14 days
|
|
Did the D5 work for LC? And was it just D5 in water or was it in D5 in .9% saline. ?
|
Shrimps
Traveler



Registered: 10/13/22
Posts: 2,501
Loc: Under the sea
|
Re: D5W for LC [Re: Bleep75]
#28093387 - 12/11/22 01:38 PM (1 year, 1 month ago) |
|
|
15 years, 21 days ago was the last post. I wouldn't give much hope in a reply ^^
|
Screwup
Googles your dumb questions


Registered: 01/27/22
Posts: 6,299
Last seen: 8 minutes, 5 seconds
|
Re: D5W for LC [Re: Bleep75] 1
#28093423 - 12/11/22 02:10 PM (1 year, 1 month ago) |
|
|
Quote:
Bleep75 said: Did the D5 work for LC? And was it just D5 in water or was it in D5 in .9% saline. ?
Fifteen years ago You think they gonna respond?
Edited by Screwup (12/11/22 02:10 PM)
|
|