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djsollest
workin hard



Registered: 08/25/07
Posts: 121
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question about microscope objectives
#7671881 - 11/23/07 06:42 PM (16 years, 2 months ago) |
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Ok heres the deal
I'm looking at two different microscopes and need to pick one.
Would it be the same basic thing if I get the microscope that has 4x 10x 40x & 100x for objectives, and the other only has 4x 10x 40x, but to get the 1000x could I just buy the 25x eye piece. Will the clarity be the same? Heres the links for the two different microscopes. need to pick one... what would you do?
http://store.amscope.com/m220.html http://store.amscope.com/m600.html
Also I know these aren't the best microscopes but they are about the best I could find in my budget.
-------------------- "DRUM N BASS IN YER FACE" "a love to sleep with opium dreams" My first grow! Brazilians with H poo / Coir http://www.shroomery.org/forums/showflat.php/Number/7730494#7730494
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: question about microscope objectives [Re: djsollest]
#7672010 - 11/23/07 07:06 PM (16 years, 2 months ago) |
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I don't care much for either one of those. The lenses are achromatic as opposed to plan achromatic, which keeps your object in focus over the entire objective. With achromatic, you'll have a part in the center of the lens that is in focus only. In addition, budget scopes have very small objectives, so they don't gather much light.
What is the main purpose of a scope for you? I use my zoom microscope most of all. It is designed to look at things from the top, using reflected light from a lamp also above the culture, rather than looking at the light that shines through your object from a lamp below. I wouldn't recommend 20X eyepeices. They block too much light, and their optics are usually poor, especially on the made in china microscopes. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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djsollest
workin hard



Registered: 08/25/07
Posts: 121
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Re: question about microscope objectives [Re: RogerRabbit]
#7672122 - 11/23/07 07:22 PM (16 years, 2 months ago) |
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ok thank you for the info... I will be using the microscope for viewing spores, bacteria rods, and other basic mycology / microbiology things. Also I'm a vet tek and will be using it to look at fecal matter, sperm and protozoans. Hey this company does offer those "plan achromatic objectives" and better 30mm eye pieces. Would this scope be much better if I was to get those instead of the ones it came with? thank you very much for you help RR.
-------------------- "DRUM N BASS IN YER FACE" "a love to sleep with opium dreams" My first grow! Brazilians with H poo / Coir http://www.shroomery.org/forums/showflat.php/Number/7730494#7730494
Edited by djsollest (11/23/07 07:24 PM)
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djsollest
workin hard



Registered: 08/25/07
Posts: 121
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Re: question about microscope objectives [Re: djsollest]
#7672170 - 11/23/07 07:31 PM (16 years, 2 months ago) |
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One more thing, what is Phase Contrast objectives?
Thank you again
-------------------- "DRUM N BASS IN YER FACE" "a love to sleep with opium dreams" My first grow! Brazilians with H poo / Coir http://www.shroomery.org/forums/showflat.php/Number/7730494#7730494
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RogerRabbit
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: question about microscope objectives [Re: djsollest]
#7672215 - 11/23/07 07:37 PM (16 years, 2 months ago) |
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If you can get plan lenses, get them. The 30 mm eyepieces are great. I have a few sets of those for my scopes. I'd suggest getting one with a 100X oil emersion objective. If you want to count nuclei in a cell, you'll probably need it.
You can google terms such as plan, phase contrast, dark field, anchromatic, etc. for lots of reading. Here's a page that tells a bit about phase contrast. http://www.microscopeworld.com/MSWorld/phase.aspx
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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djsollest
workin hard



Registered: 08/25/07
Posts: 121
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Re: question about microscope objectives [Re: RogerRabbit]
#7672344 - 11/23/07 08:00 PM (16 years, 2 months ago) |
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Awsome, will do more reading.
I appreciate the help
-------------------- "DRUM N BASS IN YER FACE" "a love to sleep with opium dreams" My first grow! Brazilians with H poo / Coir http://www.shroomery.org/forums/showflat.php/Number/7730494#7730494
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asci
HONGO

Registered: 03/22/06
Posts: 111
Loc: oregon
Last seen: 2 years, 6 months
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Re: question about microscope objectives [Re: djsollest]
#7677846 - 11/25/07 12:58 PM (16 years, 2 months ago) |
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the phase contrast is the main system i use for mycological and microbiological study in the lab. phase contrast are best used if your observing live cells. as for needing 1000x viewable objective? maybe for the smaller prokaryotic cell structures, but for the mycological observation i rarely need something that powerful. look into a nikon scope, the extra cost is well worth it!
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