Do I want vented or non-vented petri dishes? - Gourmet and Medicinal Mushrooms

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Jeremy_Davis
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Registered: 04/22/05
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Loc: Florida
Last seen: 12 years, 6 days

Do I want vented or non-vented petri dishes?

#7664006 - 11/21/07 05:32 PM (16 years, 4 months ago)

When I was buying them last time, I don't remember what I bought.

I found the below at a commercial site;

Some of our dish models are available in both "vented" and "non-vented" styles. Standard Petri Dishes are always vented, so if the don't say vented or non-vented, you should assume they are vented. "Vented" means that the lid is slightly elevated above the base. This allows for good, plentiful air exchange. This is useful when you want to encourage evaporation, for example, when you want to use poured plates as soon as possible, and the plates themselves, or a liquid seeding solution, needs to dry beforehand. The basic design of the dish tends to maintain sterility because particles would have to go up and over the dish's wall to get inside, and this is rare in normal airflow. With "non-vented" dishes, the lid fits quite flatly on the base. While it is not a hermetic seal, the space between dish and lid is extremely small. This results in even less potential for external contamination and a significantly reduced evaporation rate. For example a 60mm vented Petri Dish containing 10ml of agar medium typically dries out in 2-3 weeks; whereas, a similar 60mm non-vented dish typically lasts 2-3 months. Most C. elegans labs, except those in very humid climates, prefer the non-vented dishes. Non-vented dishes provide sufficient air exchange for the worms to breath while greatly increasing the life of the dish.

Looks like we can use either one. I always wrap petris with parafilm anyway...

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Jeremy Davis
Educational Concerns for Hunger Organization, Inc.
Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta

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RogerRabbit
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Re: Do I want vented or non-vented petri dishes?

[Re: Jeremy_Davis]

1
#7666399 - 11/22/07 09:51 AM (16 years, 4 months ago)

Vented. The mycelium needs the gas exchange, and your parafilm will help to prevent drying. If you use non-vented, you still need to wrap with parafilm, but then gas exchange is reduced to the point that growth stalls. I had to donate 3/4 case of non-vented dishes to a local high school because they just wouldn't work properly. Growth would stall after a few days, and bacteria is encouraged.
RR

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Jeremy_Davis
Mycelial NetworkAdministrator

Registered: 04/22/05
Posts: 652
Loc: Florida
Last seen: 12 years, 6 days

Re: Do I want vented or non-vented petri dishes?

[Re: RogerRabbit]
#7666830 - 11/22/07 12:22 PM (16 years, 4 months ago)

Thanks man! Just the info I was looking for.

--------------------
Jeremy Davis
Educational Concerns for Hunger Organization, Inc.
Check out the ECHO mushroom blog page to see our lab, growing facility, and more-www.echotech.org/greta

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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms

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