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hightimesreader
Half assed question asker



Registered: 07/18/06
Posts: 2,543
Loc: In the air conditioning
Last seen: 11 years, 10 months
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How apt is this to fail?
#7661673 - 11/21/07 06:11 AM (16 years, 2 months ago) |
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Okay, I'm out of spores, and was stoned, so about 2 nights ago, I threw together 9 rye berry jars and forgot that I have no spores. Whats a mycologist to do? Well, I improvised. I know that you can transfer live tissue of a mushroom onto an agar plate and it'll grow out the tissue. So, I collect a few good sized pins/mature mushrooms, picked, dryed, and a few, I cut a section of the base off and placed in to the jar to innoc. 2 days, and no signs of growth. Will it work out? I dont see it being THAT far out...
HTR
-------------------- I'm hunting for The Following ethnos. For experiments, hunting finds and any other contributions, check out My journal. HTR A new leaf turned over.. I'm too old for this shit.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Most likely contam. When taking clones, u want a clean peice of tissue, usually a scraping from the middle of the stem, placed on agar or in an LC, but even then it sometimes contams.
Soaking in bleach for a few minutes is a good way of cleaning a tissue sample.
You would be better making up an LC from a tissue sample then using that to inoculate your jars.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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hightimesreader
Half assed question asker



Registered: 07/18/06
Posts: 2,543
Loc: In the air conditioning
Last seen: 11 years, 10 months
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Re: How apt is this to fail? [Re: veda_sticks]
#7661709 - 11/21/07 06:32 AM (16 years, 2 months ago) |
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Quote:
veda_sticks said: Most likely contam. When taking clones, u want a clean peice of tissue, usually a scraping from the middle of the stem, placed on agar or in an LC, but even then it sometimes contams.
Soaking in bleach for a few minutes is a good way of cleaning a tissue sample.
You would be better making up an LC from a tissue sample then using that to inoculate your jars.
Lol, shit... I dipped it in peroxide
-------------------- I'm hunting for The Following ethnos. For experiments, hunting finds and any other contributions, check out My journal. HTR A new leaf turned over.. I'm too old for this shit.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 25 days
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Bleach solution is much better for this purpose as it doesnt really harm mycelium, compared to peroxide which can cause quite a shock.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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