Home | Community | Message Board

Avalon Magic Plants
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinehightimesreader
Half assed question asker
 User Gallery


Registered: 07/18/06
Posts: 2,543
Loc: In the air conditioning
Last seen: 11 years, 10 months
How apt is this to fail?
    #7661673 - 11/21/07 06:11 AM (16 years, 2 months ago)

Okay, I'm out of spores, and was stoned, so about 2 nights ago, I threw together 9 rye berry jars and forgot that I have no spores. Whats a mycologist to do? Well, I improvised. I know that you can transfer live tissue of a mushroom onto an agar plate and it'll grow out the tissue. So, I collect a few good sized pins/mature mushrooms, picked, dryed, and a few, I cut a section of the base off and placed in to the jar to innoc. 2 days, and no signs of growth. Will it work out? I dont see it being THAT far out...

HTR


--------------------
I'm hunting for The Following ethnos.
For experiments, hunting finds and any other contributions, check out My journal.
HTR

A new leaf turned over.. I'm too old for this shit.
:commonsense:


Extras: Filter Print Post Top
Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: How apt is this to fail? [Re: hightimesreader]
    #7661678 - 11/21/07 06:14 AM (16 years, 2 months ago)

Most likely contam. When taking clones, u want a clean peice of tissue, usually a scraping from the middle of the stem, placed on agar or in an LC, but even then it sometimes contams.

Soaking in bleach for a few minutes is a good way of cleaning a tissue sample.

You would be better making up an LC from a tissue sample then using that to inoculate your jars.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
Offlinehightimesreader
Half assed question asker
 User Gallery


Registered: 07/18/06
Posts: 2,543
Loc: In the air conditioning
Last seen: 11 years, 10 months
Re: How apt is this to fail? [Re: veda_sticks]
    #7661709 - 11/21/07 06:32 AM (16 years, 2 months ago)

Quote:

veda_sticks said:
Most likely contam. When taking clones, u want a clean peice of tissue, usually a scraping from the middle of the stem, placed on agar or in an LC, but even then it sometimes contams.

Soaking in bleach for a few minutes is a good way of cleaning a tissue sample.

You would be better making up an LC from a tissue sample then using that to inoculate your jars.




Lol, shit...  I dipped it in peroxide :smile:


--------------------
I'm hunting for The Following ethnos.
For experiments, hunting finds and any other contributions, check out My journal.
HTR

A new leaf turned over.. I'm too old for this shit.
:commonsense:


Extras: Filter Print Post Top
Offlineveda_sticks
Cultivator
Male User Gallery


Folding@home Statistics
Registered: 07/29/07
Posts: 14,191
Loc: UK Flag
Last seen: 4 years, 25 days
Re: How apt is this to fail? [Re: hightimesreader]
    #7661712 - 11/21/07 06:34 AM (16 years, 2 months ago)

Bleach solution is much better for this purpose as it doesnt really harm mycelium, compared to peroxide which can cause quite a shock.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* Why Does it fail After entering FC? marrow444 1,338 8 04/16/07 05:18 PM
by FriendlyNeighbor
* Peroxide Syringe Darkflame 450 2 11/09/05 04:13 PM
by ready2go
* 3 failed grain attempts, please help :(
( 1 2 3 all )
RootedGrowth 5,948 42 03/07/05 09:58 AM
by scatmanrav
* 2nd run at agar failed again :( makaveli8x8 878 7 03/19/07 11:37 AM
by RogerRabbit
* Can I add certain % Peroxide to substrate mix? Numba9 8,334 5 08/23/01 09:31 AM
by shellacct
* Re: Peroxide to kill flies on shrooms and casing. Help !!! DinoMyc 933 2 01/05/00 12:28 PM
by DinoMyc
* Peroxide Precaution? stoner2002 1,752 6 07/11/07 04:24 PM
by figgusfiddus
* Peroxide is Bullcrap.
( 1 2 all )
boneynerd 7,580 20 08/21/11 09:06 PM
by ambrosia_uecharist

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
261 topic views. 13 members, 149 guests and 43 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.011 seconds on 14 queries.