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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
invitro bags....dunk or spray between flushes?
    #7659603 - 11/20/07 04:17 PM (16 years, 2 months ago)

Should I dunk or is a good spray sufficient?


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Offlinehaxjester
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Re: invitro bags....dunk or spray between flushes? [Re: WWorker]
    #7660049 - 11/20/07 05:53 PM (16 years, 2 months ago)

A good spray is all that is necessary.  :courtjester:

I look at what I've gone through in my career and I really shouldn't be talking to you now. I should be dead ten times over. -Jack


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: invitro bags....dunk or spray between flushes? [Re: WWorker]
    #7660081 - 11/20/07 06:00 PM (16 years, 2 months ago)

if you just spray, it'll have to be one hell of a spray.
like at least half a cups worth.


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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
Re: invitro bags....dunk or spray between flushes? [Re: monstermitch]
    #7660202 - 11/20/07 06:33 PM (16 years, 2 months ago)

so.....


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: invitro bags....dunk or spray between flushes? [Re: WWorker]
    #7660307 - 11/20/07 06:58 PM (16 years, 2 months ago)

so....


it works both ways.
I've done both hundreds of times with near identical success.

after the first flush I find misting so much that there's about
a half inch of water left in the bag is about right. the sub should
suck it right up in no time.
as the flushes progress, less misting/spraying is needed, as the
sub can suck up less and less water as it ages.

I wouldn't be surprised if I misted a whole cup of water after the
first flush. maybe a half after the second. a quarter after the third.
and so on...
per bag that is. per 5 pound bag that is.

granted, substrate composition has a lot to do with it.
if you have an inferior substrate to the one I use, you won't be
able to mist as much. some substrates are far and above others
in terms of holding and delivering water. it's one of the most
important characteristics of a substrate.
so results on amounts will vary.

the point is you can do either with equal success if it's done right.
the misting/spraying will take longer to get the feel for how it
works best for you.
dunking is harder, takes longer, but works the same about
every time. it's real hard to mess up dunking.


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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
Re: invitro bags....dunk or spray between flushes? [Re: monstermitch]
    #7660438 - 11/20/07 07:45 PM (16 years, 2 months ago)

Thanks for the added info....would it be possible to put some water in the bag and then pour off the excess after a time period...an in-the-bag dunking?


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: invitro bags....dunk or spray between flushes? [Re: WWorker]
    #7660453 - 11/20/07 07:50 PM (16 years, 2 months ago)

yes.

any time you have standing water at all it's cause for worry.
so if it doesn't absorb quickly, then you'll want to dump it out.

what you're talking about works for bulk subs too.
floating I guess you can call it.

fill up the tub/tray/bag/whatever until the sub floats, then let
it float for several hours and dump the water out.

adding a bit of ice when doing this may be a good idea.
it'll help keep the water under 40 degrees to prevent bacteria
from blooming.


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Offlineozzyozzyozzy
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Re: invitro bags....dunk or spray between flushes? [Re: monstermitch]
    #7660496 - 11/20/07 08:06 PM (16 years, 2 months ago)

Is there any special way to induce invitro fruiting in a spawn bag? I'm getting a lot of rhizomorphic growth on the bottom half, it seems to have stalled at that point though and I can't get any pins to form.


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Invisiblemonstermitch
Growing in Bags Doesn't Work

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Re: invitro bags....dunk or spray between flushes? [Re: ozzyozzyozzy]
    #7660713 - 11/20/07 09:12 PM (16 years, 2 months ago)

Quote:

ozzyozzyozzy said:
Is there any special way to induce invitro fruiting in a spawn bag? I'm getting a lot of rhizomorphic growth on the bottom half, it seems to have stalled at that point though and I can't get any pins to form.




they will come. be patient.

if you mixed at 25% colonization or whatever, and the substrate
has since fully colonized, pins will form.
it may take up to two weeks though from first sign of full colonization.

it's just one of those things you'll have to forget about or not think
too much about. it'll come.


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Offlineozzyozzyozzy
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Re: invitro bags....dunk or spray between flushes? [Re: monstermitch]
    #7660743 - 11/20/07 09:22 PM (16 years, 2 months ago)

While I'm waiting [and not positive it's fully colonized] do I just leave it in the bag, sealed off? Or do I want FAE [opened top or fanning three times a day], a lot of light, misting etc?


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Offlineacommunistspy
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Re: invitro bags....dunk or spray between flushes? *DELETED* [Re: ozzyozzyozzy]
    #7660764 - 11/20/07 09:29 PM (16 years, 2 months ago)

Post deleted by acommunistspy

Reason for deletion: this never happened



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Offlineozzyozzyozzy
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Re: invitro bags....dunk or spray between flushes? [Re: acommunistspy]
    #7660811 - 11/20/07 09:44 PM (16 years, 2 months ago)

Hmm alright. I'll leave it on the closet outside of light and just neglect it for a while. It has enough moisture, the rhizo should mean it's not contammed. I fear I've mucked around with it too much so I'll knock up some more bags and leave that one for a week or more to see if anything happens. Thanks.


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Offlineacommunistspy
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Re: invitro bags....dunk or spray between flushes? *DELETED* [Re: ozzyozzyozzy]
    #7660893 - 11/20/07 10:13 PM (16 years, 2 months ago)

Post deleted by acommunistspy

Reason for deletion: this never happened



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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
Re: invitro bags....dunk or spray between flushes? [Re: acommunistspy]
    #7661603 - 11/21/07 04:31 AM (16 years, 2 months ago)

Thanks MM and commy...lol


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Offlineozzyozzyozzy
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Re: invitro bags....dunk or spray between flushes? [Re: WWorker]
    #7664103 - 11/21/07 06:01 PM (16 years, 2 months ago)

The top half is slowly becoming more rhizomorphic, but not colonized properly - it seems very dry [even though the bag is wet] and the entire substrate has pulled away from the bag edges. Should I leave it longer to colonize [since the bottom half and increasing is rhizo] or should I spray some water to help it along?


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Offlineozzyozzyozzy
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Re: invitro bags....dunk or spray between flushes? [Re: ozzyozzyozzy]
    #7664150 - 11/21/07 06:15 PM (16 years, 2 months ago)

Some photos: [Sorry I didn't upload to shroomery.org but they automatically resize and thus lose a lot of detail].








It just seems like the seeds have lost all their water-content and the myc is going rhizomorphic to absorb the water accumulating as condensation on the bag walls.


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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
Re: invitro bags....dunk or spray between flushes? [Re: ozzyozzyozzy] * 1
    #7664172 - 11/21/07 06:19 PM (16 years, 2 months ago)

Did you break it up to mix up the myc growth?

if you haven't. it might be a good idea to do so on the uncolonized bags.

The top looks fully colonized.


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Offlineozzyozzyozzy
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Re: invitro bags....dunk or spray between flushes? [Re: WWorker]
    #7664194 - 11/21/07 06:24 PM (16 years, 2 months ago)

Those are all photos of the same bag. I broke up the substrate several times over the colonization period. It's just not connecting the colonization on top even though I haven't broken it up recently, so I'm a bit worried it's lost its will to live.


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InvisibleWWorker
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Registered: 09/09/07
Posts: 395
Re: invitro bags....dunk or spray between flushes? [Re: ozzyozzyozzy]
    #7664254 - 11/21/07 06:37 PM (16 years, 2 months ago)

What temps are you incubating at?

You could put a dollop of silicon on the bag, sterilize a syringe fullof clean water and squirt a few cc's into the bag and then mix up.

I did this to a couple of jars that started looking dry and it seemed to help.


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Offlineozzyozzyozzy
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Re: invitro bags....dunk or spray between flushes? [Re: WWorker]
    #7664277 - 11/21/07 06:41 PM (16 years, 2 months ago)

I wouldn't bother with the silicon or sterile water, normal tap water put into a spray mist should be alright if I don't put too much in. The mycelium, as you said, looks pretty well colonized so the chances of competing contams getting a foothold is greatly reduced.

I'm incubating at room temperature in my closet, so probably 20-24 degrees Celsius [say 71F].

I just don't want to overhydrate or destroy humidity and put it back several days if the rhizo is doing alright on its own.


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